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Article

MicroRNA-1290 promotes asiatic acid‑induced apoptosis by decreasing BCL2 protein level in A549 non‑small cell lung carcinoma cells

  • Authors:
    • Ki Bbeum Kim
    • Karam Kim
    • Seunghee Bae
    • Yeonghmin Choi
    • Hwa Jun Cha
    • Soo Yeon Kim
    • Jae Ho Lee
    • So Hyeon Jeon
    • Ho Jung Jung
    • Kyu Joong Ahn
    • In-Sook An
    • Sungkwan An
  • View Affiliations / Copyright

    Affiliations: Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143‑701, Republic of Korea, Laboratory of Molecular Oncology, Cheil General Hospital and Women's Healthcare Center, Kwandong University, College of Medicine, Seoul 100‑380, Republic of Korea, Department of Beauty Design, Dongwon University, Gwangju, Gyeonggi‑do, 464-711, Republic of Korea, Department of Dermatology, Konkuk University School of Medicine, Seoul 143-701, Republic of Korea
  • Pages: 1029-1036
    |
    Published online on: July 10, 2014
       https://doi.org/10.3892/or.2014.3319
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Abstract

Asiatic acid, a triterpenoid derived from Centella asiatica, is a putative anticancer agent in several types of cancer cells. Investigations of its biological role in negative regulation of cell growth have focused on the extent of induction of apoptosis in a cell-type-specific manner. In this study, we identified an important regulator of asiatic acid-induced cell death, microRNA (miR)-1290, which sensitizes cells to asiatic acid-induced cytotoxicity and negatively regulates BCL2 expression. Asiatic acid significantly upregulated miR-1290, and asiatic acid-induced cell death was shown to be dependent on miR-1290 activity. Molecular assays demonstrated that BCL2 mRNA is a direct target of miR-1290-mediated RNA interference. The results of functional studies suggest that miR-1290 suppresses cell viability and cell cycle progression. These data provide insight into miR-1290-mediated cellular mechanisms in asiatic acid-treated A549 non-small cell lung carcinoma cells.
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Copy and paste a formatted citation
Spandidos Publications style
Kim KB, Kim K, Bae S, Choi Y, Cha HJ, Kim SY, Lee JH, Jeon SH, Jung HJ, Ahn KJ, Ahn KJ, et al: MicroRNA-1290 promotes asiatic acid‑induced apoptosis by decreasing BCL2 protein level in A549 non‑small cell lung carcinoma cells. Oncol Rep 32: 1029-1036, 2014.
APA
Kim, K.B., Kim, K., Bae, S., Choi, Y., Cha, H.J., Kim, S.Y. ... An, S. (2014). MicroRNA-1290 promotes asiatic acid‑induced apoptosis by decreasing BCL2 protein level in A549 non‑small cell lung carcinoma cells. Oncology Reports, 32, 1029-1036. https://doi.org/10.3892/or.2014.3319
MLA
Kim, K. B., Kim, K., Bae, S., Choi, Y., Cha, H. J., Kim, S. Y., Lee, J. H., Jeon, S. H., Jung, H. J., Ahn, K. J., An, I., An, S."MicroRNA-1290 promotes asiatic acid‑induced apoptosis by decreasing BCL2 protein level in A549 non‑small cell lung carcinoma cells". Oncology Reports 32.3 (2014): 1029-1036.
Chicago
Kim, K. B., Kim, K., Bae, S., Choi, Y., Cha, H. J., Kim, S. Y., Lee, J. H., Jeon, S. H., Jung, H. J., Ahn, K. J., An, I., An, S."MicroRNA-1290 promotes asiatic acid‑induced apoptosis by decreasing BCL2 protein level in A549 non‑small cell lung carcinoma cells". Oncology Reports 32, no. 3 (2014): 1029-1036. https://doi.org/10.3892/or.2014.3319
Copy and paste a formatted citation
x
Spandidos Publications style
Kim KB, Kim K, Bae S, Choi Y, Cha HJ, Kim SY, Lee JH, Jeon SH, Jung HJ, Ahn KJ, Ahn KJ, et al: MicroRNA-1290 promotes asiatic acid‑induced apoptosis by decreasing BCL2 protein level in A549 non‑small cell lung carcinoma cells. Oncol Rep 32: 1029-1036, 2014.
APA
Kim, K.B., Kim, K., Bae, S., Choi, Y., Cha, H.J., Kim, S.Y. ... An, S. (2014). MicroRNA-1290 promotes asiatic acid‑induced apoptosis by decreasing BCL2 protein level in A549 non‑small cell lung carcinoma cells. Oncology Reports, 32, 1029-1036. https://doi.org/10.3892/or.2014.3319
MLA
Kim, K. B., Kim, K., Bae, S., Choi, Y., Cha, H. J., Kim, S. Y., Lee, J. H., Jeon, S. H., Jung, H. J., Ahn, K. J., An, I., An, S."MicroRNA-1290 promotes asiatic acid‑induced apoptosis by decreasing BCL2 protein level in A549 non‑small cell lung carcinoma cells". Oncology Reports 32.3 (2014): 1029-1036.
Chicago
Kim, K. B., Kim, K., Bae, S., Choi, Y., Cha, H. J., Kim, S. Y., Lee, J. H., Jeon, S. H., Jung, H. J., Ahn, K. J., An, I., An, S."MicroRNA-1290 promotes asiatic acid‑induced apoptosis by decreasing BCL2 protein level in A549 non‑small cell lung carcinoma cells". Oncology Reports 32, no. 3 (2014): 1029-1036. https://doi.org/10.3892/or.2014.3319
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