MicroRNA-1290 promotes asiatic acid‑induced apoptosis by decreasing BCL2 protein level in A549 non‑small cell lung carcinoma cells

  • Authors:
    • Ki Bbeum Kim
    • Karam Kim
    • Seunghee Bae
    • Yeonghmin Choi
    • Hwa Jun Cha
    • Soo Yeon Kim
    • Jae Ho Lee
    • So Hyeon Jeon
    • Ho Jung Jung
    • Kyu Joong Ahn
    • In-Sook An
    • Sungkwan An
  • View Affiliations

  • Published online on: July 10, 2014     https://doi.org/10.3892/or.2014.3319
  • Pages: 1029-1036
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Asiatic acid, a triterpenoid derived from Centella asiatica, is a putative anticancer agent in several types of cancer cells. Investigations of its biological role in negative regulation of cell growth have focused on the extent of induction of apoptosis in a cell-type-specific manner. In this study, we identified an important regulator of asiatic acid-induced cell death, microRNA (miR)-1290, which sensitizes cells to asiatic acid-induced cytotoxicity and negatively regulates BCL2 expression. Asiatic acid significantly upregulated miR-1290, and asiatic acid-induced cell death was shown to be dependent on miR-1290 activity. Molecular assays demonstrated that BCL2 mRNA is a direct target of miR-1290-mediated RNA interference. The results of functional studies suggest that miR-1290 suppresses cell viability and cell cycle progression. These data provide insight into miR-1290-mediated cellular mechanisms in asiatic acid-treated A549 non-small cell lung carcinoma cells.
View Figures
View References

Related Articles

Journal Cover

September-2014
Volume 32 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Kim KB, Kim K, Bae S, Choi Y, Cha HJ, Kim SY, Lee JH, Jeon SH, Jung HJ, Ahn KJ, Ahn KJ, et al: MicroRNA-1290 promotes asiatic acid‑induced apoptosis by decreasing BCL2 protein level in A549 non‑small cell lung carcinoma cells. Oncol Rep 32: 1029-1036, 2014
APA
Kim, K.B., Kim, K., Bae, S., Choi, Y., Cha, H.J., Kim, S.Y. ... An, S. (2014). MicroRNA-1290 promotes asiatic acid‑induced apoptosis by decreasing BCL2 protein level in A549 non‑small cell lung carcinoma cells. Oncology Reports, 32, 1029-1036. https://doi.org/10.3892/or.2014.3319
MLA
Kim, K. B., Kim, K., Bae, S., Choi, Y., Cha, H. J., Kim, S. Y., Lee, J. H., Jeon, S. H., Jung, H. J., Ahn, K. J., An, I., An, S."MicroRNA-1290 promotes asiatic acid‑induced apoptosis by decreasing BCL2 protein level in A549 non‑small cell lung carcinoma cells". Oncology Reports 32.3 (2014): 1029-1036.
Chicago
Kim, K. B., Kim, K., Bae, S., Choi, Y., Cha, H. J., Kim, S. Y., Lee, J. H., Jeon, S. H., Jung, H. J., Ahn, K. J., An, I., An, S."MicroRNA-1290 promotes asiatic acid‑induced apoptosis by decreasing BCL2 protein level in A549 non‑small cell lung carcinoma cells". Oncology Reports 32, no. 3 (2014): 1029-1036. https://doi.org/10.3892/or.2014.3319