Knockdown of nuclear factor erythroid 2-related factor 2 by lentivirus induces differentiation of glioma stem-like cells

  • Authors:
    • Jianhong Zhu
    • Handong Wang
    • Youwu Fan
    • Yangchun  Hu
    • Xiangjun Ji
    • Qing Sun
    • Huandong Liu
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  • Published online on: July 10, 2014     https://doi.org/10.3892/or.2014.3320
  • Pages: 1170-1178
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Abstract

Glioma stem cells (GSCs) are key in the progression and recurrence of glioblastoma. Inducing the differentiation of GSCs is an important therapeutic target for glioblastoma. Nuclear factor erythroid 2-related factor 2 (Nrf2) has been reported to be important in maintaining the stem cell status of GSCs; however, its association with differentiation has not been studied. Herein, we knocked down Nrf2 from GSCs to investigate the role of Nrf2 in the differentiation of GSCs. First, Nrf2 expression was observed at different stages of differentiation; then, Nrf2 was knocked down and the association of Nrf2 with differentiation degree was observed in vitro. Finally, GSCs were planted in nude mice to study the association of Nrf2 with differentiation in vivo. The expression of Nrf2 decreased with the differentiation process. Following Nrf2 knockdown, the proportion of sphere-like colonies decreased and the dendritic cells in spheres increased; the expression of Nrf2 significantly decreased while the expression of differentiation marker glial fibrillary acidic protein (GFAP) and βIII-tubulin increased both at the protein and the gene level. In the xenografts of nude mice, the differentiation of tumor cells was improved. These results suggest that Nrf2 is a key factor inhibiting the differentiation of GSCs, and knockdown of Nrf2 may promote the differentiation process, providing a therapy target for GSCs.
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September-2014
Volume 32 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhu J, Wang H, Fan Y, Hu Y, Ji X, Sun Q and Liu H: Knockdown of nuclear factor erythroid 2-related factor 2 by lentivirus induces differentiation of glioma stem-like cells. Oncol Rep 32: 1170-1178, 2014
APA
Zhu, J., Wang, H., Fan, Y., Hu, Y., Ji, X., Sun, Q., & Liu, H. (2014). Knockdown of nuclear factor erythroid 2-related factor 2 by lentivirus induces differentiation of glioma stem-like cells. Oncology Reports, 32, 1170-1178. https://doi.org/10.3892/or.2014.3320
MLA
Zhu, J., Wang, H., Fan, Y., Hu, Y., Ji, X., Sun, Q., Liu, H."Knockdown of nuclear factor erythroid 2-related factor 2 by lentivirus induces differentiation of glioma stem-like cells". Oncology Reports 32.3 (2014): 1170-1178.
Chicago
Zhu, J., Wang, H., Fan, Y., Hu, Y., Ji, X., Sun, Q., Liu, H."Knockdown of nuclear factor erythroid 2-related factor 2 by lentivirus induces differentiation of glioma stem-like cells". Oncology Reports 32, no. 3 (2014): 1170-1178. https://doi.org/10.3892/or.2014.3320