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Article

Notch-1 regulates proliferation and differentiation of human bladder cancer cell lines by inhibiting expression of Krüppel-like factor 4

  • Authors:
    • Xing Ai
    • Zhuomin Jia
    • Shuanglin Liu
    • Jiajun Wang
    • Xu Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Military General Hospital of Beijing PLA, Beijing 100700, P.R. China, Department of Urology, Wuhan First Hospital, Wuhan, Hubei 430000, P.R. China, Department of Urology, Chinese PLA General Hospital, Beijing 100853, P.R. China
  • Pages: 1459-1464
    |
    Published online on: July 23, 2014
       https://doi.org/10.3892/or.2014.3350
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Abstract

Inhibition of Notch signaling pathways, consisting of 4 highly conserved receptors (Notch 1-4), induces expression of Krüppel-like transcription factors (KLFs) linked to bladder cancer tumorigenesis and metastasis. Effects of Notch-1 knockdown on cell proliferation, differentiation and KLF4 levels in bladder cancer cell lines were investigated. PsiRNA1‑mediated Notch-1 and KLF4 knockdown models and control model without the psiRNA1 vector were constructed using bladder cancer cell lines T24 and BIU87. Cell proliferation, apoptosis and expression of Notch-1 and KLF4 were assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry assay with Annexin V-FITC/PI staining, and reverse transcriptase polymerase chain reaction (RT-PCR) and western blot analysis, respectively. Proliferation was assessed in Notch-1 and/or KLF4 knockdown. The results showed that Notch-1 mRNA and protein expression levels were significantly lower following psiRNA1 vector transfection in both cell lines (P<0.05). Growth and proliferation of both cell lines were significantly inhibited by Notch-1 knockdown (P<0.05), and more G0/G1 arrest and apoptosis were observed compared to those in the control groups (P<0.05). The effects were time-dependent, peaking between 24-48 h and declining by 72 h. KLF4 expression was significantly higher in the Notch-1 knockdown group than in control cells (P<0.05). Notch-1 knockdown cell proliferation was significantly lower than that of Notch-1 and KLF4 knockdown (P<0.05). In conclusion, Notch-1 may act as an oncogene, regulating the proliferation and differentiation of bladder cancer cells by inhibiting KLF4. Pending further exploration of pathway variations and crosstalk, these pathways may be useful targets for bladder cancer therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Ai X, Jia Z, Liu S, Wang J and Zhang X: Notch-1 regulates proliferation and differentiation of human bladder cancer cell lines by inhibiting expression of Krüppel-like factor 4. Oncol Rep 32: 1459-1464, 2014.
APA
Ai, X., Jia, Z., Liu, S., Wang, J., & Zhang, X. (2014). Notch-1 regulates proliferation and differentiation of human bladder cancer cell lines by inhibiting expression of Krüppel-like factor 4. Oncology Reports, 32, 1459-1464. https://doi.org/10.3892/or.2014.3350
MLA
Ai, X., Jia, Z., Liu, S., Wang, J., Zhang, X."Notch-1 regulates proliferation and differentiation of human bladder cancer cell lines by inhibiting expression of Krüppel-like factor 4". Oncology Reports 32.4 (2014): 1459-1464.
Chicago
Ai, X., Jia, Z., Liu, S., Wang, J., Zhang, X."Notch-1 regulates proliferation and differentiation of human bladder cancer cell lines by inhibiting expression of Krüppel-like factor 4". Oncology Reports 32, no. 4 (2014): 1459-1464. https://doi.org/10.3892/or.2014.3350
Copy and paste a formatted citation
x
Spandidos Publications style
Ai X, Jia Z, Liu S, Wang J and Zhang X: Notch-1 regulates proliferation and differentiation of human bladder cancer cell lines by inhibiting expression of Krüppel-like factor 4. Oncol Rep 32: 1459-1464, 2014.
APA
Ai, X., Jia, Z., Liu, S., Wang, J., & Zhang, X. (2014). Notch-1 regulates proliferation and differentiation of human bladder cancer cell lines by inhibiting expression of Krüppel-like factor 4. Oncology Reports, 32, 1459-1464. https://doi.org/10.3892/or.2014.3350
MLA
Ai, X., Jia, Z., Liu, S., Wang, J., Zhang, X."Notch-1 regulates proliferation and differentiation of human bladder cancer cell lines by inhibiting expression of Krüppel-like factor 4". Oncology Reports 32.4 (2014): 1459-1464.
Chicago
Ai, X., Jia, Z., Liu, S., Wang, J., Zhang, X."Notch-1 regulates proliferation and differentiation of human bladder cancer cell lines by inhibiting expression of Krüppel-like factor 4". Oncology Reports 32, no. 4 (2014): 1459-1464. https://doi.org/10.3892/or.2014.3350
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