Phloroglucinol induces apoptosis via apoptotic signaling pathways in HT-29 colon cancer cells

Kang,Mi-Hye; Kim,In-Hye; Nam,Taek-Jeong

doi:10.3892/or.2014.3355

Phloroglucinol induces apoptosis via apoptotic signaling pathways in HT-29 colon cancer cells

Authors:
- Mi-Hye Kang
- In-Hye Kim
- Taek-Jeong Nam
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Affiliations: Department of Food and Life Science, Pukyong National University, Nam-gu, Busan 608-737, Republic of Korea
Published online on: July 23, 2014 https://doi.org/10.3892/or.2014.3355
Pages: 1341-1346
Copyright: © Kang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Phloroglucinol is a polyphenolic compound that is used to treat and prevent several human diseases, as it exerts beneficial biological activities, including anti-oxidant, anti‑inflammatory and anticancer properties. The aim of the present study was to investigate the effects of phloroglucinol on apoptotic signaling pathways in HT-29 colon cancer cells. The results indicated that phloroglucinol suppressed cell viability and induced apoptosis in HT-29 cells in a concentration-dependent manner. Phloroglucinol treatment of HT-29 cells resulted in characteristic apoptosis-related changes: altered Bcl-2 family proteins, cytochrome c release, and activation of caspase-3 and caspase-8. This study also showed that proteins involved in apoptosis were stimulated by treatment with phloroglucinol. These findings demonstrated that phloroglucinol exerts anticancer activity in HT-29 colon cancer cells through induction of apoptosis.

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