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Article

Reduced expression of liver kinase B1 and Beclin1 is associated with the poor survival of patients with non-small cell lung cancer

  • Authors:
    • Lili Jiang
    • Xuan Liang
    • Mengjie Liu
    • Wenjuan Wang
    • Jiequn Ma
    • Qianqian Guo
    • Lili Han
    • Chengcheng Yang
    • Kejun Nan
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, The First Affiliated Hospital of the Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
  • Pages: 1931-1938
    |
    Published online on: August 21, 2014
       https://doi.org/10.3892/or.2014.3432
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Abstract

Liver kinase B1 (LKB1) regulates cell polarity and has tumor-suppressor functions, while Beclin1 regulates autophagy and is associated with tumorigenesis. The present study quantified the expression level of LKB1 and Beclin1 in non-small cell lung cancer (NSCLC) tissue specimens and examined the possible association with clinicopathological characteristics and the survival of patients. Quantitative real-time reverse transcriptase‑polymerase chain reaction (qRT-PCR), western blotting and immunohistochemistry were used to assess expression of LKB1 and Beclin1 in 142 NSCLC tissue samples. The data revealed that expression levels of both LKB1 and Beclin1 mRNA and protein were significantly reduced in the NSCLC tissues compared to levels in the matched surrounding normal lung tissues, and expression of LKB1 protein was associated with Beclin1 expression in the NSCLC tissues. Reduced expression of LKB1 protein was significantly associated with tumor histology (P<0.001) and poor differentiation (P<0.001), but was not associated with tumor lymph node metastasis, TNM stage or patient gender and age, while the reduced expression of Beclin1 protein was associated with tumor histology (P<0.05) and poor differentiation (P<0.05). Univariate analysis revealed that adenocarcinoma, lymph node metastasis, advanced TNM stage and reduced expression of LKB1 and Beclin1 proteins are associated with the survival of NSCLC patients. Multivariate analysis indicated that adenocarcinoma (P<0.05), lymph node metastasis (P<0.05), advanced TNM stage (P<0.001) and reduced expression of LKB1 (P<0.05) and Beclin1 (P<0.001) are all independent prognostic indicators for the survival of NSCLC patients.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang L, Liang X, Liu M, Wang W, Ma J, Guo Q, Han L, Yang C and Nan K: Reduced expression of liver kinase B1 and Beclin1 is associated with the poor survival of patients with non-small cell lung cancer. Oncol Rep 32: 1931-1938, 2014.
APA
Jiang, L., Liang, X., Liu, M., Wang, W., Ma, J., Guo, Q. ... Nan, K. (2014). Reduced expression of liver kinase B1 and Beclin1 is associated with the poor survival of patients with non-small cell lung cancer. Oncology Reports, 32, 1931-1938. https://doi.org/10.3892/or.2014.3432
MLA
Jiang, L., Liang, X., Liu, M., Wang, W., Ma, J., Guo, Q., Han, L., Yang, C., Nan, K."Reduced expression of liver kinase B1 and Beclin1 is associated with the poor survival of patients with non-small cell lung cancer". Oncology Reports 32.5 (2014): 1931-1938.
Chicago
Jiang, L., Liang, X., Liu, M., Wang, W., Ma, J., Guo, Q., Han, L., Yang, C., Nan, K."Reduced expression of liver kinase B1 and Beclin1 is associated with the poor survival of patients with non-small cell lung cancer". Oncology Reports 32, no. 5 (2014): 1931-1938. https://doi.org/10.3892/or.2014.3432
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang L, Liang X, Liu M, Wang W, Ma J, Guo Q, Han L, Yang C and Nan K: Reduced expression of liver kinase B1 and Beclin1 is associated with the poor survival of patients with non-small cell lung cancer. Oncol Rep 32: 1931-1938, 2014.
APA
Jiang, L., Liang, X., Liu, M., Wang, W., Ma, J., Guo, Q. ... Nan, K. (2014). Reduced expression of liver kinase B1 and Beclin1 is associated with the poor survival of patients with non-small cell lung cancer. Oncology Reports, 32, 1931-1938. https://doi.org/10.3892/or.2014.3432
MLA
Jiang, L., Liang, X., Liu, M., Wang, W., Ma, J., Guo, Q., Han, L., Yang, C., Nan, K."Reduced expression of liver kinase B1 and Beclin1 is associated with the poor survival of patients with non-small cell lung cancer". Oncology Reports 32.5 (2014): 1931-1938.
Chicago
Jiang, L., Liang, X., Liu, M., Wang, W., Ma, J., Guo, Q., Han, L., Yang, C., Nan, K."Reduced expression of liver kinase B1 and Beclin1 is associated with the poor survival of patients with non-small cell lung cancer". Oncology Reports 32, no. 5 (2014): 1931-1938. https://doi.org/10.3892/or.2014.3432
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