Expression and roles of fatty acid synthase in hepatocellular carcinoma

Hao,Qiwei; Li,Tao; Zhang,Xiong; Gao,Ping; Qiao,Peiyu; Li,Sheng; Geng,Zhimin

doi:10.3892/or.2014.3484

December-2014 Volume 32 Issue 6

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December-2014 Volume 32 Issue 6

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Article

Expression and roles of fatty acid synthase in hepatocellular carcinoma

Authors:
- Qiwei Hao
- Tao Li
- Xiong Zhang
- Ping Gao
- Peiyu Qiao
- Sheng Li
- Zhimin Geng
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Affiliations: Department of Hepatobiliary Surgery, Second Hospital of Yulin City, Yulin, Shaanxi 719000, P.R. China, Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, College of Medicine, Xi'an, Shaanxi 710061, P.R. China
Pages: 2471-2476
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Published online on: September 16, 2014

https://doi.org/10.3892/or.2014.3484
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Abstract

Cell metabolism abnormalities are closely related to tumor occurrence and development. Fatty acid synthase (FASN) is the key molecule for catalyzing fatty acid synthesis. Increasing evidence indicates that FASN is highly expressed in a number of malignant tumors; it can promote the synthesis of endogenous fatty acids in tumor cells and then the synthesized fatty acids provide energy for the proliferation of tumor cells. However, there has been no systematic study focusing on FASN expression and function in hepatocellular carcinoma (HCC). The aim of the present study was to verify the high expression of FASN in HCC cells at the histological and cellular levels, and to construct FASN shRNA eukaryotic expression vector for interfering FASN expression in HCC cell line SK-Hep-1, in an effort to explore the role of FASN in the proliferation, apoptosis, invasion and migration of HCC cells. In the present study, we demonstrated that FASN was highly expressed in HCC tissues compared with tumor-adjacent tissue and normal liver cell line 7702 (P<0.05). FASN expression in the high metastatic MHCC97H and SK-Hep-1 cell lines was increased compared with low metastatic HCC cell lines (P<0.05). Then, we constructed a FASN shRNA eukaryotic expression vector; after HCC SK-Hep-1 cells were transfected, the cell proliferation, migration and invasion were inhibited, but FASN had no impact on the apoptosis of HCC cells. Collectively, these data indicate that FASN is possibly involved in the occurrence and metastasis of HCC. Thus, inhibition of FASN may be a promising approach for the treatment of HCC.

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1	Daker M, Bhuvanendran S, Ahmad M, Takada K and Khoo AS: Deregulation of lipid metabolism pathway genes in nasopharyngeal carcinoma cells. Mol Med Rep. 7:731–741. 2013.PubMed/NCBI
2	Grunt TW, Wagner R, Grusch M, et al: Interaction between fatty acid synthase- and ErbB-systems in ovarian cancer cells. Biochem Biophys Res Commun. 385:454–459. 2009. View Article : Google Scholar : PubMed/NCBI
3	Zhou Y, Niu C, Li Y, et al: Fatty acid synthase expression and esophageal cancer. Mol Biol Rep. 39:9733–9739. 2012. View Article : Google Scholar : PubMed/NCBI
4	Cerne D, Zitnik IP and Sok M: Increased fatty acid synthase activity in non-small cell lung cancer tissue is a weaker predictor of shorter patient survival than increased lipoprotein lipase activity. Arch Med Res. 41:405–409. 2010. View Article : Google Scholar : PubMed/NCBI
5	Cairns RA, Harris I, McCracken S and Mak TW: Cancer cell metabolism. Cold Spring Harb Symp Quant Biol. 76:299–311. 2011. View Article : Google Scholar
6	Glaysher J: Lipid metabolism and cancer. Curr Opin Lipidol. 24:530–531. 2013. View Article : Google Scholar
7	Kuhajda FP: Fatty-acid synthase and human cancer: new perspectives on its role in tumor biology. Nutrition. 16:202–208. 2000. View Article : Google Scholar : PubMed/NCBI
8	Pflug BR, Pecher SM, Brink AW, Nelson JB and Foster BA: Increased fatty acid synthase expression and activity during progression of prostate cancer in the TRAMP model. Prostate. 57:245–254. 2003. View Article : Google Scholar : PubMed/NCBI
9	Swinnen JV, Roskams T, Joniau S, et al: Overexpression of fatty acid synthase is an early and common event in the development of prostate cancer. Int J Cancer. 98:19–22. 2002. View Article : Google Scholar : PubMed/NCBI
10	Horiguchi A, Asano T, Asano T, Ito K, Sumitomo M and Hayakawa M: Fatty acid synthase over expression is an indicator of tumor aggressiveness and poor prognosis in renal cell carcinoma. J Urol. 180:1137–1140. 2008. View Article : Google Scholar : PubMed/NCBI
11	Sugino T, Baba K, Hoshi N, Aikawa K, Yamaquchi O and Suzuki T: Overexpression of fatty acid synthase in human urinary bladder cancer and combined expression of the synthase and Ki-67 as a predictor of prognosis of cancer patients. Med Mol Morphol. 44:146–150. 2011. View Article : Google Scholar : PubMed/NCBI
12	Pizer ES, Kurman RJ, Pasternack GR and Kuhajda FP: Expression of fatty acid synthase is closely linked to proliferation and stromal decidualization in cycling endometrium. Int J Gynecol Pathol. 16:45–51. 1997. View Article : Google Scholar : PubMed/NCBI
13	Liu ZL, Zhou Y, Luo QF, et al: Inhibition of fatty acid synthase supresses osteosarcoma cell invasion and migration. Indian J Pathol Microbiol. 55:163–169. 2012. View Article : Google Scholar : PubMed/NCBI
14	Long XH, Mao JH, Peng AF, Zhou Y, Huang SH and Liu ZL: Tumor suppressive microRNA-424 inhibits osteosarcoma cell migration and invasion via targeting fatty acid synthase. Exp Ther Med. 5:1048–1052. 2013.PubMed/NCBI
15	Alli PM, Pinn ML, Jaffee EM, McFadden JM and Kuhajda FP: Fatty acid synthase inhibitors are chemopreventive for mammary cancer in neu-N transgenic mice. Oncogene. 24:39–46. 2005. View Article : Google Scholar : PubMed/NCBI
16	Menendez JA, Mehmi I, Verma VA, Teng PK and Lupu R: Pharmacological inhibition of fatty acid synthase (FAS): a novel therapeutic approach for breast cancer chemoprevention through its ability to suppress Her-2/neu (erbB-2) oncogene-induced malignant transformation. Mol Carcinog. 41:164–178. 2004. View Article : Google Scholar
17	Pandey PR, Liu W, Xing F, Fukuda K and Watabe K: Anti-cancer drugs targeting fatty acid synthase (FAS). Recent Pat Anticancer Drug Discov. 7:185–197. 2012. View Article : Google Scholar : PubMed/NCBI
18	Calvisi DF, Wang C, Ho C, et al: Increased lipogenesis, induced by AKT-mTORC1-RPS6 signaling, promotes development of human hepatocellular carcinoma. Gastroenterology. 140:1071–1083. 2011. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Expression and roles of fatty acid synthase in hepatocellular carcinoma

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Abstract

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