Fasudil inhibits prostate cancer-induced angiogenesis in vitro

  • Authors:
    • Weihua Chen
    • Kaili Mao
    • Thong Hua-Huy
    • Yihua Bei
    • Zhongmin Liu
    • Anh-Tuan Dinh-Xuan
  • View Affiliations

  • Published online on: September 17, 2014     https://doi.org/10.3892/or.2014.3491
  • Pages: 2795-2802
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Abstract

Inhibition of angiogenesis is an important therapeutic strategy for advanced stage prostate cancer (PCa). RhoA/Rho-associated protein kinases (ROCK) are key regulators of the cytoskeleton and have been implicated in PCa angiogenesis. We investigated the anti-angiogenic effects of fasudil, a ROCK inhibitor, on PCa-induced angiogenesis in vitro. Proliferation of PCa-conditioned human umbilical vein endothelial cells (HUVECs) was assessed using a bromodeoxyuridine (BrdU) assay, and migration was assessed with a wound healing assay. In vitro angiogenesis of PCa-conditioned HUVECs was evaluated by tube formation and a spheroid sprouting assay. Fasudil inhibited PCa-induced endothelial cell proliferation at a concentration of 100 µM, and also decreased PCa-induced endothelial cell migration at a concentration of 30 µM. In the in vitro angiogenesis assay, fasudil exerted a more significant effect. Tube formation was significantly inhibited at fasudil concentrations exceeding 3 µM, and spheroid sprouts were significantly thinner and shorter (at fasudil concentrations of 10 and 30 µM, respectively). Western blotting results showed that expression of phosphorylated myosin phosphatase target subunit 1 (MYPT-1) was significantly lower after fasudil treatment, confirming that fasudil inhibited ROCK activity in these model systems. These data suggest that fasudil may be a useful anti-angiogenic agent for PCa.
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December-2014
Volume 32 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Chen W, Mao K, Hua-Huy T, Bei Y, Liu Z and Dinh-Xuan A: Fasudil inhibits prostate cancer-induced angiogenesis in vitro. Oncol Rep 32: 2795-2802, 2014
APA
Chen, W., Mao, K., Hua-Huy, T., Bei, Y., Liu, Z., & Dinh-Xuan, A. (2014). Fasudil inhibits prostate cancer-induced angiogenesis in vitro. Oncology Reports, 32, 2795-2802. https://doi.org/10.3892/or.2014.3491
MLA
Chen, W., Mao, K., Hua-Huy, T., Bei, Y., Liu, Z., Dinh-Xuan, A."Fasudil inhibits prostate cancer-induced angiogenesis in vitro". Oncology Reports 32.6 (2014): 2795-2802.
Chicago
Chen, W., Mao, K., Hua-Huy, T., Bei, Y., Liu, Z., Dinh-Xuan, A."Fasudil inhibits prostate cancer-induced angiogenesis in vitro". Oncology Reports 32, no. 6 (2014): 2795-2802. https://doi.org/10.3892/or.2014.3491