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Expression of genes encoding extracellular matrix proteins: A macroarray study

  • Authors:
    • Konrad Futyma
    • Paweł Miotła
    • Krystyna  Różyńska
    • Małgorzata Zdunek
    • Andrzej  Semczuk
    • Tomasz Rechberger
    • Jacek Wojcierowski
  • View Affiliations / Copyright

    Affiliations: Second Department of Gynecology, Medical University of Lublin, Lublin, Poland, Department of Clinical Genetics, Medical University of Lublin, Lublin, Poland, Department of Clinical Pathology, Medical University of Lublin, Lublin, Poland, Department of Medical Genetics, Medical University of Lublin, Lublin, Poland
    Copyright: © Futyma et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 2349-2353
    |
    Published online on: September 17, 2014
       https://doi.org/10.3892/or.2014.3493
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Abstract

Endometrial cancer (EC) is one of the most common gynecological malignancies in Poland, with well-established risk factors. Genetic instability and molecular alterations responsible for endometrial carcinogenesis have been systematically investigated. The aim of the present study was to investigate, by means of cDNA macroarrays, the expression profiles of genes encoding extracellular matrix (ECM) proteins in ECs. Tissue specimens were collected during surgical procedures from 40 patients with EC, and control tissue was collected from 9 patients with uterine leiomyomas. RNA was isolated and RT-PCR with radioisotope-labeled cDNA was performed. The levels of ECM protein gene expression in normal endometrial tissues were compared to the expression of these genes in EC specimens. Statistically significant differences in gene expression, stratified by clinical stage of the ECs, were detected for aggrecan, vitronectin, tenascin R, nidogen and two collagen proteins: type VIII chain α1 and type XI chain α2. All of these proteins were overexpressed in stage III endometrial carcinomas compared to levels in stage I and II uterine neoplasms. In conclusion, increased expression of genes encoding ECM proteins may play an important role in facilitating accelerated disease progression of human ECs.
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Copy and paste a formatted citation
Spandidos Publications style
Futyma K, Miotła P, Różyńska K, Zdunek M, Semczuk A, Rechberger T and Wojcierowski J: Expression of genes encoding extracellular matrix proteins: A macroarray study. Oncol Rep 32: 2349-2353, 2014.
APA
Futyma, K., Miotła, P., Różyńska, K., Zdunek, M., Semczuk, A., Rechberger, T., & Wojcierowski, J. (2014). Expression of genes encoding extracellular matrix proteins: A macroarray study. Oncology Reports, 32, 2349-2353. https://doi.org/10.3892/or.2014.3493
MLA
Futyma, K., Miotła, P., Różyńska, K., Zdunek, M., Semczuk, A., Rechberger, T., Wojcierowski, J."Expression of genes encoding extracellular matrix proteins: A macroarray study". Oncology Reports 32.6 (2014): 2349-2353.
Chicago
Futyma, K., Miotła, P., Różyńska, K., Zdunek, M., Semczuk, A., Rechberger, T., Wojcierowski, J."Expression of genes encoding extracellular matrix proteins: A macroarray study". Oncology Reports 32, no. 6 (2014): 2349-2353. https://doi.org/10.3892/or.2014.3493
Copy and paste a formatted citation
x
Spandidos Publications style
Futyma K, Miotła P, Różyńska K, Zdunek M, Semczuk A, Rechberger T and Wojcierowski J: Expression of genes encoding extracellular matrix proteins: A macroarray study. Oncol Rep 32: 2349-2353, 2014.
APA
Futyma, K., Miotła, P., Różyńska, K., Zdunek, M., Semczuk, A., Rechberger, T., & Wojcierowski, J. (2014). Expression of genes encoding extracellular matrix proteins: A macroarray study. Oncology Reports, 32, 2349-2353. https://doi.org/10.3892/or.2014.3493
MLA
Futyma, K., Miotła, P., Różyńska, K., Zdunek, M., Semczuk, A., Rechberger, T., Wojcierowski, J."Expression of genes encoding extracellular matrix proteins: A macroarray study". Oncology Reports 32.6 (2014): 2349-2353.
Chicago
Futyma, K., Miotła, P., Różyńska, K., Zdunek, M., Semczuk, A., Rechberger, T., Wojcierowski, J."Expression of genes encoding extracellular matrix proteins: A macroarray study". Oncology Reports 32, no. 6 (2014): 2349-2353. https://doi.org/10.3892/or.2014.3493
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