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Article

microRNA sponge blocks the tumor-suppressing functions of microRNA-122 in human hepatoma and osteosarcoma cells

  • Authors:
    • Ji Ma
    • Qi Wu
    • Yue Zhang
    • Jinghua Li
    • Yongchun Yu
    • Qiuhui Pan
    • Fenyong Sun
  • View Affiliations / Copyright

    Affiliations: Central Laboratory, Shanghai Tenth People's Hospital of Tongji University, Shanghai 200072, P.R. China, Department of Medical Laboratory, Shanghai Tenth People's Hospital of Tongji University, Shanghai 200072, P.R. China
  • Pages: 2744-2752
    |
    Published online on: September 25, 2014
       https://doi.org/10.3892/or.2014.3517
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Abstract

microRNAs (miRNAs), as gene expression regulators, have been identified to be closely associated with tumorigenesis. Thus a loss-of-function study is more likely to reveal the biological roles of endogenous miRNAs. Genetic knockout, antisense oligonucleotide inhibitors, and miRNA sponge (miR‑SP) are usually performed to inhibit the activities of miRNAs of interest. In the present study, we utilized the miR-SP method, which has long-term rather than short-term effects of antisense oligonucleotide inhibitors, to generate a microRNA-122 sponge (miR-122-SP) mediated by lentivirus, and identified its silencing role in the Huh7 hepatoma cell line and U2OS osteosarcoma cell line. The results showed that miR-122-SP effectively sequestered ectopic miR-122 and restored the expression of miR-122 which targets cyclin G1 (CCNG1), Bcl-w and disintegrin and metalloprotease 10. Moreover, miR-122-SP overexpression rescued the effects of ectopic miR-122 on suppressing proliferation, inhibiting cell migration and invasion, arresting cell cycle at G1 phase, and activating caspase-3/7, not only in Huh7 human hepatoma cells, but also in U2OS osteosarcoma cells. miR-122-SP also knocked down endogenous miR-122 expression in Huh7 and promoted tumorigenesis in vivo. miR-122-SP therefore is a useful tool that may be utilized to study the functions of miR-122 with regard to liver development and tumorigenesis in vitro and in vivo.
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1 

Calin GA and Croce CM: MicroRNA signatures in human cancers. Nat Rev Cancer. 6:857–866. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Garzon R, Fabbri M, Cimmino A, Calin GA and Croce CM: MicroRNA expression and function in cancer. Trends Mol Med. 12:580–587. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Zeng Y: Principles of micro-RNA production and maturation. Oncogene. 25:6156–6162. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Valencia-Sanchez MA, Liu J, Hannon GJ and Parker R: Control of translation and mRNA degradation by miRNAs and siRNAs. Genes Dev. 20:515–524. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Ahmed FE: Role of miRNA in carcinogenesis and biomarker selection: a methodological view. Expert Rev Mol Diagn. 7:569–603. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Chen CZ: MicroRNAs as oncogenes and tumor suppressors. N Engl J Med. 353:1768–1771. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Croce CM: Oncogenes and cancer. N Engl J Med. 358:502–511. 2008. View Article : Google Scholar

8 

Bushati N and Cohen SM: microRNA functions. Annu Rev Cell Dev Biol. 23:175–205. 2007. View Article : Google Scholar

9 

Ebert MS and Sharp PA: MicroRNA sponges: progress and possibilities. RNA. 16:2043–2050. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Brown BD and Naldini L: Exploiting and antagonizing microRNA regulation for therapeutic and experimental applications. Nat Rev Genet. 10:578–585. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Kloosterman WP, Lagendijk AK, Ketting RF, Moulton JD and Plasterk RH: Targeted inhibition of miRNA maturation with morpholinos reveals a role for miR-375 in pancreatic islet development. PLoS Biol. 5:e2032007. View Article : Google Scholar : PubMed/NCBI

12 

Krützfeldt J, Rajewsky N, Braich R, et al: Silencing of microRNAs in vivo with ‘antagomirs’. Nature. 438:685–689. 2005.

13 

Park CY, Choi YS and McManus MT: Analysis of microRNA knockouts in mice. Hum Mol Genet. 19:R169–R175. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Ebert MS, Neilson JR and Sharp PA: MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells. Nat Methods. 4:721–726. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Barbato C, Ruberti F, Pieri M, et al: MicroRNA-92 modulates K(+) Cl(−) co-transporter KCC2 expression in cerebellar granule neurons. J Neurochem. 113:591–600. 2010.PubMed/NCBI

16 

Huang J, Zhao L, Xing L and Chen D: MicroRNA-204 regulates Runx2 protein expression and mesenchymal progenitor cell differentiation. Stem Cells. 28:357–364. 2010.PubMed/NCBI

17 

Bonci D, Coppola V, Musumeci M, et al: The miR-15a-miR-16-1 cluster controls prostate cancer by targeting multiple oncogenic activities. Nat Med. 14:1271–1277. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Valastyan S, Reinhardt F, Benaich N, et al: A pleiotropically acting microRNA, miR-31, inhibits breast cancer metastasis. Cell. 137:1032–1046. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Papapetrou EP, Korkola JE and Sadelain M: A genetic strategy for single and combinatorial analysis of miRNA function in mammalian hematopoietic stem cells. Stem Cells. 28:287–296. 2010.PubMed/NCBI

20 

Gentner B, Schira G, Giustacchini A, et al: Stable knockdown of microRNA in vivo by lentiviral vectors. Nat Methods. 6:63–66. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Loya CM, Lu CS, Van Vactor D and Fulga TA: Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms. Nat Methods. 6:897–903. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W and Tuschl T: Identification of tissue-specific microRNAs from mouse. Curr Biol. 12:735–739. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Landgraf P, Rusu M, Sheridan R, et al: A mammalian microRNA expression atlas based on small RNA library sequencing. Cell. 129:1401–1414. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Jopling CL, Norman KL and Sarnow P: Positive and negative modulation of viral and cellular mRNAs by liver-specific microRNA miR-122. Cold Spring Harb Symp Quant Biol. 71:369–376. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Jopling CL: Regulation of hepatitis C virus by microRNA-122. Biochem Soc Trans. 36:1220–1223. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Girard M, Jacquemin E, Munnich A, Lyonnet S and Henrion-Caude A: miR-122, a paradigm for the role of microRNAs in the liver. J Hepatol. 48:648–656. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Gramantieri L, Ferracin M, Fornari F, et al: Cyclin G1 is a target of miR-122a, a microRNA frequently down-regulated in human hepatocellular carcinoma. Cancer Res. 67:6092–6099. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Ma L, Liu J, Shen J, et al: Expression of miR-122 mediated by adenoviral vector induces apoptosis and cell cycle arrest of cancer cells. Cancer Biol Ther. 9:554–561. 2010. View Article : Google Scholar : PubMed/NCBI

29 

Bai S, Nasser MW, Wang B, et al: MicroRNA-122 inhibits tumorigenic properties of hepatocellular carcinoma cells and sensitizes these cells to sorafenib. J Biol Chem. 284:32015–32027. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Wang S, Qiu L, Yan X, et al: Loss of microRNA 122 expression in patients with hepatitis B enhances hepatitis B virus replication through cyclin G(1)-modulated P53 activity. Hepatology. 55:730–741. 2012. View Article : Google Scholar

31 

Lin CJ, Gong HY, Tseng HC, Wang WL and Wu JL: miR-122 targets an anti-apoptotic gene, Bcl-w, in human hepatocellular carcinoma cell lines. Biochem Biophys Res Commun. 375:315–320. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Tsai WC, Hsu PW, Lai TC, et al: MicroRNA-122, a tumor suppressor microRNA that regulates intrahepatic metastasis of hepatocellular carcinoma. Hepatology. 49:1571–1582. 2009. View Article : Google Scholar : PubMed/NCBI

33 

Kutay H, Bai S, Datta J, et al: Downregulation of miR-122 in the rodent and human hepatocellular carcinomas. J Cell Biochem. 99:671–678. 2006. View Article : Google Scholar : PubMed/NCBI

34 

Sahu SC, O’Donnell MW Jr and Sprando RL: Interactive toxicity of usnic acid and lipopolysaccharides in human liver HepG2 cells. J Appl Toxicol. 32:739–749. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Meister G, Landthaler M, Dorsett Y and Tuschl T: Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing. RNA. 10:544–550. 2004. View Article : Google Scholar : PubMed/NCBI

36 

Ørom UA, Kauppinen S and Lund AH: LNA-modified oligonucleotides mediate specific inhibition of microRNA function. Gene. 372:137–141. 2006.PubMed/NCBI

37 

Davis S, Lollo B, Freier S and Esau C: Improved targeting of miRNA with antisense oligonucleotides. Nucleic Acids Res. 34:2294–2304. 2006. View Article : Google Scholar : PubMed/NCBI

38 

Obad S, dos Santos CO, Petri A, et al: Silencing of microRNA families by seed-targeting tiny LNAs. Nat Genet. 43:371–378. 2011. View Article : Google Scholar : PubMed/NCBI

39 

Otaegi G, Pollock A and Sun T: An optimized sponge for microrna miR-9 affects spinal motor neuron development in vivo. Front Neurosci. 5:1462012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ma J, Wu Q, Zhang Y, Li J, Yu Y, Pan Q and Sun F: microRNA sponge blocks the tumor-suppressing functions of microRNA-122 in human hepatoma and osteosarcoma cells. Oncol Rep 32: 2744-2752, 2014.
APA
Ma, J., Wu, Q., Zhang, Y., Li, J., Yu, Y., Pan, Q., & Sun, F. (2014). microRNA sponge blocks the tumor-suppressing functions of microRNA-122 in human hepatoma and osteosarcoma cells. Oncology Reports, 32, 2744-2752. https://doi.org/10.3892/or.2014.3517
MLA
Ma, J., Wu, Q., Zhang, Y., Li, J., Yu, Y., Pan, Q., Sun, F."microRNA sponge blocks the tumor-suppressing functions of microRNA-122 in human hepatoma and osteosarcoma cells". Oncology Reports 32.6 (2014): 2744-2752.
Chicago
Ma, J., Wu, Q., Zhang, Y., Li, J., Yu, Y., Pan, Q., Sun, F."microRNA sponge blocks the tumor-suppressing functions of microRNA-122 in human hepatoma and osteosarcoma cells". Oncology Reports 32, no. 6 (2014): 2744-2752. https://doi.org/10.3892/or.2014.3517
Copy and paste a formatted citation
x
Spandidos Publications style
Ma J, Wu Q, Zhang Y, Li J, Yu Y, Pan Q and Sun F: microRNA sponge blocks the tumor-suppressing functions of microRNA-122 in human hepatoma and osteosarcoma cells. Oncol Rep 32: 2744-2752, 2014.
APA
Ma, J., Wu, Q., Zhang, Y., Li, J., Yu, Y., Pan, Q., & Sun, F. (2014). microRNA sponge blocks the tumor-suppressing functions of microRNA-122 in human hepatoma and osteosarcoma cells. Oncology Reports, 32, 2744-2752. https://doi.org/10.3892/or.2014.3517
MLA
Ma, J., Wu, Q., Zhang, Y., Li, J., Yu, Y., Pan, Q., Sun, F."microRNA sponge blocks the tumor-suppressing functions of microRNA-122 in human hepatoma and osteosarcoma cells". Oncology Reports 32.6 (2014): 2744-2752.
Chicago
Ma, J., Wu, Q., Zhang, Y., Li, J., Yu, Y., Pan, Q., Sun, F."microRNA sponge blocks the tumor-suppressing functions of microRNA-122 in human hepatoma and osteosarcoma cells". Oncology Reports 32, no. 6 (2014): 2744-2752. https://doi.org/10.3892/or.2014.3517
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