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Article

Restoration of miR-7 expression suppresses the growth of Lewis lung cancer cells by modulating epidermal growth factor receptor signaling

  • Authors:
    • Jingrong Li
    • Yijie Zheng
    • Gengyun Sun
    • Shudao Xiong
  • View Affiliations / Copyright

    Affiliations: Department of Emergency, The Second Hospital of Anhui Medical University, Hefei, Anhui, P.R. China, Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY, USA, Department of Respiratory Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, P.R. China, Department of Hematology/Oncology, The Second Hospital of Anhui Medical University, Hefei, Anhui, P.R. China
  • Pages: 2511-2516
    |
    Published online on: September 26, 2014
       https://doi.org/10.3892/or.2014.3519
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Abstract

microRNAs are an abundant class of short endogenous non-coding RNAs that function as important regulators of multiple target genes and participate in diverse biological roles in carcinogenesis. However, the role of miR-7 in lung cancer remains unclear and requires further elucidation. In the present study, we found a reduction of miR-7 expression in Lewis lung cancer (3LL) cells originating from mice by real-time RT-PCR. Restoration of miR-7 inhibited 3LL cell proliferation, induced cell apoptosis in vitro and reduced tumorigenicity in vivo. We further confirmed that miR-7 downregulated the expression of both epidermal growth factor receptor (EGFR) and murine leukemia viral oncogene homologue-1 (RAF-1) oncogenes by real-time PCR and western blot analysis. Furthermore, inhibition of EGFR showed similar effects to miR-7 enforcement in 3LL cells. Taken together, these findings revealed that miR-7 acts as an antitumor miRNA in 3LL by targeting and suppressing the expression of both EGFR and RAF-1 oncogenes. This study may provide a rationale for the use of miR-7 in lung cancer target therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Li J, Zheng Y, Sun G and Xiong S: Restoration of miR-7 expression suppresses the growth of Lewis lung cancer cells by modulating epidermal growth factor receptor signaling. Oncol Rep 32: 2511-2516, 2014.
APA
Li, J., Zheng, Y., Sun, G., & Xiong, S. (2014). Restoration of miR-7 expression suppresses the growth of Lewis lung cancer cells by modulating epidermal growth factor receptor signaling. Oncology Reports, 32, 2511-2516. https://doi.org/10.3892/or.2014.3519
MLA
Li, J., Zheng, Y., Sun, G., Xiong, S."Restoration of miR-7 expression suppresses the growth of Lewis lung cancer cells by modulating epidermal growth factor receptor signaling". Oncology Reports 32.6 (2014): 2511-2516.
Chicago
Li, J., Zheng, Y., Sun, G., Xiong, S."Restoration of miR-7 expression suppresses the growth of Lewis lung cancer cells by modulating epidermal growth factor receptor signaling". Oncology Reports 32, no. 6 (2014): 2511-2516. https://doi.org/10.3892/or.2014.3519
Copy and paste a formatted citation
x
Spandidos Publications style
Li J, Zheng Y, Sun G and Xiong S: Restoration of miR-7 expression suppresses the growth of Lewis lung cancer cells by modulating epidermal growth factor receptor signaling. Oncol Rep 32: 2511-2516, 2014.
APA
Li, J., Zheng, Y., Sun, G., & Xiong, S. (2014). Restoration of miR-7 expression suppresses the growth of Lewis lung cancer cells by modulating epidermal growth factor receptor signaling. Oncology Reports, 32, 2511-2516. https://doi.org/10.3892/or.2014.3519
MLA
Li, J., Zheng, Y., Sun, G., Xiong, S."Restoration of miR-7 expression suppresses the growth of Lewis lung cancer cells by modulating epidermal growth factor receptor signaling". Oncology Reports 32.6 (2014): 2511-2516.
Chicago
Li, J., Zheng, Y., Sun, G., Xiong, S."Restoration of miR-7 expression suppresses the growth of Lewis lung cancer cells by modulating epidermal growth factor receptor signaling". Oncology Reports 32, no. 6 (2014): 2511-2516. https://doi.org/10.3892/or.2014.3519
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