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Article

Annexin A5 inhibits diffuse large B-cell lymphoma cell invasion and chemoresistance through phosphatidylinositol 3-kinase signaling

  • Authors:
    • Jingjing Wang
    • Yang Zhang
    • Xianling Liu
    • Jinan Ma
    • Ping Liu
    • Chunhong Hu
    • Guangsen Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China, Department of Hematology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China
  • Pages: 2557-2563
    |
    Published online on: October 14, 2014
       https://doi.org/10.3892/or.2014.3547
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Abstract

Diffuse large B-cell lymphoma (DLBCL) is the most common type of non-Hodgkin's lymphoma worldwide. Although patient outcomes have significantly improved to a greater than 40% cure rate by the combinatorial cyclophosphamide, doxorubicin, vincristine and prednisone (CHOP) chemotherapy, which is widely used, resistance to the CHOP regimen continues to pose a problem in managing or curing DLBCL. While it promotes the malignancy and chemo­resistance in certain types of cancer, Annexin A5 is negatively correlated with those in other cancers, including DLBCL. In the present study, we explored the effects of Annexin A5 on DLBCL cell invasion and chemoresistance to CHOP. Stable overexpression and knockdown of Annexin A5 were performed in Toledo and Pfeiffer human DLBCL cell lines. Overexpression of Annexin A5 in both cell lines significantly decreased cell invasion, matrix metalloproteinase-9 (MMP-9) expression/activity, phosphatidylinositol 3-kinase (PI3K) activity/Akt phosphorylation, and cell survival against CHOP-induced apoptosis. On the other hand, knockdown of Annexin A5 markedly increased cell invasion, MMP-9 expression/activity, PI3K activity/Akt phosphorylation, and CHOP-induced apoptosis in the DLBCL cell lines, which was abolished by selective PI3K inhibitor BKM120. In conclusion, our study provides the first in vitro evidence that Annexin A5 inhibits DLBCL cell invasion, MMP-9 expression/activity, and chemoresistance to CHOP through a PI3K-dependent mechanism; it provides new insight not only into the biological function of Annexin A5, but also into the molecular mechanisms underlying DLBCL progression and chemoresistance.
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Copy and paste a formatted citation
Spandidos Publications style
Wang J, Zhang Y, Liu X, Ma J, Liu P, Hu C and Zhang G: Annexin A5 inhibits diffuse large B-cell lymphoma cell invasion and chemoresistance through phosphatidylinositol 3-kinase signaling. Oncol Rep 32: 2557-2563, 2014.
APA
Wang, J., Zhang, Y., Liu, X., Ma, J., Liu, P., Hu, C., & Zhang, G. (2014). Annexin A5 inhibits diffuse large B-cell lymphoma cell invasion and chemoresistance through phosphatidylinositol 3-kinase signaling. Oncology Reports, 32, 2557-2563. https://doi.org/10.3892/or.2014.3547
MLA
Wang, J., Zhang, Y., Liu, X., Ma, J., Liu, P., Hu, C., Zhang, G."Annexin A5 inhibits diffuse large B-cell lymphoma cell invasion and chemoresistance through phosphatidylinositol 3-kinase signaling". Oncology Reports 32.6 (2014): 2557-2563.
Chicago
Wang, J., Zhang, Y., Liu, X., Ma, J., Liu, P., Hu, C., Zhang, G."Annexin A5 inhibits diffuse large B-cell lymphoma cell invasion and chemoresistance through phosphatidylinositol 3-kinase signaling". Oncology Reports 32, no. 6 (2014): 2557-2563. https://doi.org/10.3892/or.2014.3547
Copy and paste a formatted citation
x
Spandidos Publications style
Wang J, Zhang Y, Liu X, Ma J, Liu P, Hu C and Zhang G: Annexin A5 inhibits diffuse large B-cell lymphoma cell invasion and chemoresistance through phosphatidylinositol 3-kinase signaling. Oncol Rep 32: 2557-2563, 2014.
APA
Wang, J., Zhang, Y., Liu, X., Ma, J., Liu, P., Hu, C., & Zhang, G. (2014). Annexin A5 inhibits diffuse large B-cell lymphoma cell invasion and chemoresistance through phosphatidylinositol 3-kinase signaling. Oncology Reports, 32, 2557-2563. https://doi.org/10.3892/or.2014.3547
MLA
Wang, J., Zhang, Y., Liu, X., Ma, J., Liu, P., Hu, C., Zhang, G."Annexin A5 inhibits diffuse large B-cell lymphoma cell invasion and chemoresistance through phosphatidylinositol 3-kinase signaling". Oncology Reports 32.6 (2014): 2557-2563.
Chicago
Wang, J., Zhang, Y., Liu, X., Ma, J., Liu, P., Hu, C., Zhang, G."Annexin A5 inhibits diffuse large B-cell lymphoma cell invasion and chemoresistance through phosphatidylinositol 3-kinase signaling". Oncology Reports 32, no. 6 (2014): 2557-2563. https://doi.org/10.3892/or.2014.3547
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