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Article

Combination of the mTOR inhibitor RAD001 with temozolomide and radiation effectively inhibits the growth of glioblastoma cells in culture

  • Authors:
    • Hélène Burckel
    • Elodie Josset
    • Jean-Marc Denis
    • John Gueulette
    • Jakobus Slabbert
    • Georges Noël
    • Pierre Bischoff
  • View Affiliations / Copyright

    Affiliations: EA 3430, University of Strasbourg, Centre Paul Strauss, Strasbourg, France, MIRO, Catholic University of Louvain, Brussels, Belgium, iThemba LABS, Somerset West, South Africa
  • Pages: 471-477
    |
    Published online on: November 4, 2014
       https://doi.org/10.3892/or.2014.3590
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Abstract

The present in vitro study aimed to assess the effects of combining the mTOR inhibitor RAD001 and temozolomide (TMZ) together with irradiation by either low-linear energy transfer (LET) radiation (γ-rays) or high-LET radiation (fast neutrons) on the growth and cell survival of the human glioblastoma cell line U-87. We observed a strong decrease in cell proliferation along with a concomitant increase in cell death as a function of the radiation dose. As expected, high-LET radiation was more effective and induced more sustained damage to DNA than low-LET radiation. While RAD001 in association with TMZ induced autophagic cell death, additional combination with either type of radiation did not further increase its occurrence. On the contrary, apoptosis remained at a low level in all experimental groups.
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Copy and paste a formatted citation
Spandidos Publications style
Burckel H, Josset E, Denis J, Gueulette J, Slabbert J, Noël G and Bischoff P: Combination of the mTOR inhibitor RAD001 with temozolomide and radiation effectively inhibits the growth of glioblastoma cells in culture. Oncol Rep 33: 471-477, 2015.
APA
Burckel, H., Josset, E., Denis, J., Gueulette, J., Slabbert, J., Noël, G., & Bischoff, P. (2015). Combination of the mTOR inhibitor RAD001 with temozolomide and radiation effectively inhibits the growth of glioblastoma cells in culture. Oncology Reports, 33, 471-477. https://doi.org/10.3892/or.2014.3590
MLA
Burckel, H., Josset, E., Denis, J., Gueulette, J., Slabbert, J., Noël, G., Bischoff, P."Combination of the mTOR inhibitor RAD001 with temozolomide and radiation effectively inhibits the growth of glioblastoma cells in culture". Oncology Reports 33.1 (2015): 471-477.
Chicago
Burckel, H., Josset, E., Denis, J., Gueulette, J., Slabbert, J., Noël, G., Bischoff, P."Combination of the mTOR inhibitor RAD001 with temozolomide and radiation effectively inhibits the growth of glioblastoma cells in culture". Oncology Reports 33, no. 1 (2015): 471-477. https://doi.org/10.3892/or.2014.3590
Copy and paste a formatted citation
x
Spandidos Publications style
Burckel H, Josset E, Denis J, Gueulette J, Slabbert J, Noël G and Bischoff P: Combination of the mTOR inhibitor RAD001 with temozolomide and radiation effectively inhibits the growth of glioblastoma cells in culture. Oncol Rep 33: 471-477, 2015.
APA
Burckel, H., Josset, E., Denis, J., Gueulette, J., Slabbert, J., Noël, G., & Bischoff, P. (2015). Combination of the mTOR inhibitor RAD001 with temozolomide and radiation effectively inhibits the growth of glioblastoma cells in culture. Oncology Reports, 33, 471-477. https://doi.org/10.3892/or.2014.3590
MLA
Burckel, H., Josset, E., Denis, J., Gueulette, J., Slabbert, J., Noël, G., Bischoff, P."Combination of the mTOR inhibitor RAD001 with temozolomide and radiation effectively inhibits the growth of glioblastoma cells in culture". Oncology Reports 33.1 (2015): 471-477.
Chicago
Burckel, H., Josset, E., Denis, J., Gueulette, J., Slabbert, J., Noël, G., Bischoff, P."Combination of the mTOR inhibitor RAD001 with temozolomide and radiation effectively inhibits the growth of glioblastoma cells in culture". Oncology Reports 33, no. 1 (2015): 471-477. https://doi.org/10.3892/or.2014.3590
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