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Article

Evaluation of melatonin treatment in primary culture of canine mammary tumors

  • Authors:
    • Juliana Ramos Lopes
    • Larissa Bazela Maschio
    • Bruna Victorasso Jardim-Perassi
    • Marina Gobbe Moschetta
    • Lívia Carvalho Ferreira
    • Gustavo Rodrigues Martins
    • Gabriela Bottaro Gelaleti
    • Debora Aparecida Pires De Campos Zuccari
  • View Affiliations / Copyright

    Affiliations: Post-graduate Program in Genetics, São Paulo State University (UNESP), São José do Rio Preto, SP, Brazil, Laboratory of Molecular Research in Cancer (LIMC), São José do Rio Preto, SP, Brazil
  • Pages: 311-319
    |
    Published online on: November 11, 2014
       https://doi.org/10.3892/or.2014.3596
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Abstract

Mammary neoplasias are the most common tumors observed in female dogs. Identification of these tumors is valuable in order to identify beneficial therapeutic agents as alternative treatments for this tumor type. Oral administration of melatonin appears to exert an oncostatic effect on mammary neoplasia and may have a possible mechanism of action through its interaction with estrogen receptors on epithelial cells. Hence, we analyzed the potential therapeutic value of melatonin in tumors that are estrogen-dependent or -independent, and established a relationship of its action with the expression of the melatonin receptors MT1 and MT2. Furthermore, we analyzed the rate of cell proliferation and apoptosis after treatment with melatonin. Cell cultures were performed using 10 canine mammary tumor fragments and were divided into estrogen receptor (ER)-positive and ER-negative tumors. The results showed that both ER-positive and ER-negative tumors had decreased cell viability and proliferation after treatment with melatonin (p<0.05), although treatment was more effective in the ER-positive tumors. Analysis of the relative expression of the MT1 and MT2 genes by quantitative PCR was performed and the data were compared with the expression of ER in 24 canine mammary tumors and the cellular response to melatonin in 10 samples. MT1 was overexpressed in ER-positive tumors (p<0.05), whereas MT2 was not expressed. Furthermore, melatonin treatment in ER-positive tumors showed an efficient oncostatic effect by inhibiting cell viability and proliferation and inducing apoptosis. These results suggest that melatonin decreased neoplastic mammary cell proliferation and viability and induced apoptosis, with greater efficacy in ER-positive tumors that have a high expression of melatonin receptor MT1. This is a strong evidence for the use of melatonin as a therapeutic agent for estrogen-dependent canine mammary tumors.
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Copy and paste a formatted citation
Spandidos Publications style
Lopes JR, Maschio LB, Jardim-Perassi BV, Moschetta MG, Ferreira LC, Martins GR, Gelaleti GB and De Campos Zuccari DP: Evaluation of melatonin treatment in primary culture of canine mammary tumors. Oncol Rep 33: 311-319, 2015.
APA
Lopes, J.R., Maschio, L.B., Jardim-Perassi, B.V., Moschetta, M.G., Ferreira, L.C., Martins, G.R. ... De Campos Zuccari, D.P. (2015). Evaluation of melatonin treatment in primary culture of canine mammary tumors. Oncology Reports, 33, 311-319. https://doi.org/10.3892/or.2014.3596
MLA
Lopes, J. R., Maschio, L. B., Jardim-Perassi, B. V., Moschetta, M. G., Ferreira, L. C., Martins, G. R., Gelaleti, G. B., De Campos Zuccari, D. P."Evaluation of melatonin treatment in primary culture of canine mammary tumors". Oncology Reports 33.1 (2015): 311-319.
Chicago
Lopes, J. R., Maschio, L. B., Jardim-Perassi, B. V., Moschetta, M. G., Ferreira, L. C., Martins, G. R., Gelaleti, G. B., De Campos Zuccari, D. P."Evaluation of melatonin treatment in primary culture of canine mammary tumors". Oncology Reports 33, no. 1 (2015): 311-319. https://doi.org/10.3892/or.2014.3596
Copy and paste a formatted citation
x
Spandidos Publications style
Lopes JR, Maschio LB, Jardim-Perassi BV, Moschetta MG, Ferreira LC, Martins GR, Gelaleti GB and De Campos Zuccari DP: Evaluation of melatonin treatment in primary culture of canine mammary tumors. Oncol Rep 33: 311-319, 2015.
APA
Lopes, J.R., Maschio, L.B., Jardim-Perassi, B.V., Moschetta, M.G., Ferreira, L.C., Martins, G.R. ... De Campos Zuccari, D.P. (2015). Evaluation of melatonin treatment in primary culture of canine mammary tumors. Oncology Reports, 33, 311-319. https://doi.org/10.3892/or.2014.3596
MLA
Lopes, J. R., Maschio, L. B., Jardim-Perassi, B. V., Moschetta, M. G., Ferreira, L. C., Martins, G. R., Gelaleti, G. B., De Campos Zuccari, D. P."Evaluation of melatonin treatment in primary culture of canine mammary tumors". Oncology Reports 33.1 (2015): 311-319.
Chicago
Lopes, J. R., Maschio, L. B., Jardim-Perassi, B. V., Moschetta, M. G., Ferreira, L. C., Martins, G. R., Gelaleti, G. B., De Campos Zuccari, D. P."Evaluation of melatonin treatment in primary culture of canine mammary tumors". Oncology Reports 33, no. 1 (2015): 311-319. https://doi.org/10.3892/or.2014.3596
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