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Article

Kazinol C from Broussonetia kazinoki activates AMP-activated protein kinase to induce antitumorigenic effects in HT-29 colon cancer cells

  • Authors:
    • Hak-Su Kim
    • Jihyun Lim
    • Da Yeon Lee
    • Jae-Ha Ryu
    • Jong-Seok Lim
  • View Affiliations / Copyright

    Affiliations: Department of Biological Science and the Research Center for Women's Diseases, Sookmyung Women’s University, Yongsan-Gu, Seoul 140-742, Republic of Korea, Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, Yongsan-Gu, Seoul 140-742, Republic of Korea
  • Pages: 223-229
    |
    Published online on: November 12, 2014
       https://doi.org/10.3892/or.2014.3601
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Abstract

Kazinol C is a 1,3-diphenylpropane, obtained from Broussonetia kazinoki, that has been employed in folk medicine as an edema suppressant. It exerts beneficial effects in oxidative stress-related diseases, such as cancer. However, the molecular mechanism involved in the anticancer effects remains to be determined. AMP-activated protein kinase (AMPK) has emerged as a possible anticancer target molecule. The present study investigated the effect of kazinol C on AMPK activation as well as subsequent HT-29 colon cancer cell viability, apoptosis and migration. Kazinol C markedly induced AMPK phosphorylation and significantly attenuated HT-29 colon cancer cell growth and viability. Compound C, as a well‑known AMPK inhibitor, blocked the kazinol C-induced cell death, and stable transduction of dominant-negative (DN) AMPK in colon cancer cells also inhibited kazinol C-induced cell apoptosis. In addition, kazinol C inhibited HT-29 cell migration and anchorage-independent growth. AMPK inhibition using stable transduction with DN AMPK significantly abrogated the kazinol C-induced inhibition of cancer cell migration. Thus, AMPK is a critical and novel regulator of kazinol C-mediated cancer cell apoptosis and inhibition of migration, suggesting that AMPK is a prime cancer target.
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1 

Hardie DG, Carling D and Carlson M: The AMP-activated/SNF1 protein kinase subfamily: metabolic sensors of the eukaryotic cell? Annu Rev Biochem. 67:821–855. 1998. View Article : Google Scholar : PubMed/NCBI

2 

Hardie DG and Alessi DR: LKB1 and AMPK and the cancer-metabolism link - ten years after. BMC Biol. 11:362013. View Article : Google Scholar : PubMed/NCBI

3 

Shackelford DB and Shaw RJ: The LKB1-AMPK pathway: metabolism and growth control in tumour suppression. Nat Rev Cancer. 9:563–575. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Inoki K, Ouyang H, Zhu T, Lindvall C, Wang Y, Zhang X, Yang Q, Bennett C, Harada Y, Stankunas K, Wang CY, He X, MacDougald OA, You M, Williams BO and Guan KL: TSC2 integrates Wnt and energy signals via a coordinated phosphorylation by AMPK and GSK3 to regulate cell growth. Cell. 126:955–968. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Jones RG, Plas DR, Kubek S, Buzzai M, Mu J, Xu Y, Birnbaum MJ and Thompson CB: AMP-activated protein kinase induces a p53-dependent metabolic checkpoint. Mol Cell. 18:283–293. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Hwang JT, Kim YM, Surh YJ, Baik HW, Lee SK, Ha J and Park OJ: Selenium regulates cyclooxygenase-2 and extracellular signal-regulated kinase signaling pathways by activating AMP-activated protein kinase in colon cancer cells. Cancer Res. 66:10057–10063. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Mihaylova MM and Shaw RJ: The AMPK signalling pathway coordinates cell growth, autophagy and metabolism. Nat Cell Biol. 13:1016–1023. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Sanli T, Steinberg GR, Singh G and Tsakiridis T: AMP-activated protein kinase (AMPK) beyond metabolism: a novel genomic stress sensor participating in the DNA damage response pathway. Cancer Biol Ther. 15:156–169. 2014. View Article : Google Scholar :

9 

Kim HS, Kim MJ, Kim EJ, Yang Y, Lee MS and Lim JS: Berberine-induced AMPK activation inhibits the metastatic potential of melanoma cells via reduction of ERK activity and COX-2 protein expression. Biochem Pharmacol. 83:385–394. 2012. View Article : Google Scholar

10 

Nakano A, Kato H, Watanabe T, Min KD, Yamazaki S, Asano Y, Seguchi O, Higo S, Shintani Y, Asanuma H, Asakura M, Minamino T, Kaibuchi K, Mochizuki N, Kitakaze M and Takashima S: AMPK controls the speed of microtubule polymerization and directional cell migration through CLIP-170 phosphorylation. Nat Cell Biol. 12:583–590. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Taliaferro-Smith L, Nagalingam A, Zhong D, Zhou W, Saxena NK and Sharma D: LKB1 is required for adiponectin-mediated modulation of AMPK-S6K axis and inhibition of migration and invasion of breast cancer cells. Oncogene. 28:2621–2633. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Kim AY, Lee CG, Lee da Y, Li H, Jeon R, Ryu JH and Kim SG: Enhanced antioxidant effect of prenylated polyphenols as Fyn inhibitor. Free Radic Biol Med. 53:1198–1208. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Ko HH, Yen MH, Wu RR, Won SJ and Lin CN: Cytotoxic isoprenylated flavans of Broussonetia kazinoki. J Nat Prod. 62:164–166. 1999. View Article : Google Scholar : PubMed/NCBI

14 

Cha JY, Kim YT, Kim HS and Cho YS: Antihyperglycemic effect of stem bark powder from paper mulberry (Broussonetia kazinoki Sieb.) in type 2 diabetic Otsuka Long-Evans Tokushima fatty rats. J Med Food. 11:499–505. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Baek YS, Ryu YB, Curtis-Long MJ, Ha TJ, Rengasamy R, Yang MS and Park KH: Tyrosinase inhibitory effects of 1,3-diphenylpropanes from Broussonetia kazinoki. Bioorg Med Chem. 17:35–41. 2009. View Article : Google Scholar

16 

Lee DY, Kim DH, Lee HJ, Lee Y, Ryu KH, Jung BI, Song YS and Ryu JH: New estrogenic compounds isolated from Broussonetia kazinoki. Bioorg Med Chem Lett. 20:3764–3767. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Ikuta J, Hano Y, Nomura T, Kawakami Y and Sato T: Components of Broussonetia kazinoki SIEB. I Structures of two new isoprenylated flavans and five new isoprenylated 1,3-diphenylpropane derivatives. Chem Pharm Bull. 34:1968–1979. 1986. View Article : Google Scholar

18 

Woods A, Azzout-Marniche D, Foretz M, Stein SC, Lemarchand P, Ferré P, Foufelle F and Carling D: Characterization of the role of AMP-activated protein kinase in the regulation of glucose-activated gene expression using constitutively active and dominant negative forms of the kinase. Mol Cell Biol. 20:6704–6711. 2000. View Article : Google Scholar : PubMed/NCBI

19 

Wang W and Guan KL: AMP-activated protein kinase and cancer. Acta Physiol. 196:55–63. 2009. View Article : Google Scholar

20 

Jang T, Calaoagan JM, Kwon E, Samuelsson S, Recht L and Laderoute KR: 5′-AMP-activated protein kinase activity is elevated early during primary brain tumor development in the rat. Int J Cancer. 128:2230–2239. 2011. View Article : Google Scholar

21 

Yun H, Kim HS, Lee S, Kang I, Kim SS, Choe W and Ha J: AMP kinase signaling determines whether c-Jun N-terminal kinase promotes survival or apoptosis during glucose deprivation. Carcinogenesis. 30:529–537. 2009. View Article : Google Scholar

22 

Kim HS, Wannatung T, Lee S, Yang WK, Chung SH, Lim JS, Choe W, Kang I, Kim SS and Ha J: Quercetin enhances hypoxia-mediated apoptosis via direct inhibition of AMPK activity in HCT116 colon cancer. Apoptosis. 17:938–949. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Tseng CH: Diabetes, metformin use, and colon cancer: a population-based cohort study in Taiwan. Eur J Endocrinol. 167:409–416. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Din FV, Valanciute A, Houde VP, Zibrova D, Green KA, Sakamoto K, Alessi DR and Dunlop MG: Aspirin inhibits mTOR signaling, activates AMP-activated protein kinase, and induces autophagy in colorectal cancer cells. Gastroenterology. 142:1504–1515.e3. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Hawley SA, Fullerton MD, Ross FA, Schertzer JD, Chevtzoff C, Walker KJ, Peggie MW, Zibrova D, Green KA, Mustard KJ, Kemp BE, Sakamoto K, Steinberg GR and Hardie DG: The ancient drug salicylate directly activates AMP-activated protein kinase. Science. 336:918–922. 2012. View Article : Google Scholar : PubMed/NCBI

26 

O'Neill LA and Hardie DG: Metabolism of inflammation limited by AMPK and pseudo-starvation. Nature. 493:346–355. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Lee YK and Park OJ: Regulation of mutual inhibitory activities between AMPK and Akt with quercetin in MCF-7 breast cancer cells. Oncol Rep. 24:1493–1497. 2010.PubMed/NCBI

28 

Yang X and Huang N: Berberine induces selective apoptosis through the AMPK-mediated mitochondrial/caspase pathway in hepatocellular carcinoma. Mol Med Rep. 8:505–510. 2013.PubMed/NCBI

29 

Puissant A, Robert G, Fenouille N, Luciano F, Cassuto JP, Raynaud S and Auberger P: Resveratrol promotes autophagic cell death in chronic myelogenous leukemia cells via JNK-mediated p62/SQSTM1 expression and AMPK activation. Cancer Res. 70:1042–1052. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Kim EK, Park JM, Lim S, Choi JW, Kim HS, Seok H, Seo JK, Oh K, Lee DS, Kim KT, Ryu SH and Suh PG: Activation of AMP-activated protein kinase is essential for lysophosphatidic acid-induced cell migration in ovarian cancer cells. J Biol Chem. 286:24036–24045. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Frigo DE, Howe MK, Wittmann BM, Brunner AM, Cushman I, Wang Q, Brown M, Means AR and McDonnell DP: CaM kinase kinase β-mediated activation of the growth regulatory kinase AMPK is required for androgen-dependent migration of prostate cancer cells. Cancer Res. 71:528–537. 2011. View Article : Google Scholar :

32 

Ouchi N, Kobayashi H, Kihara S, Kumada M, Sato K, Inoue T, Funahashi T and Walsh K: Adiponectin stimulates angiogenesis by promoting cross-talk between AMP-activated protein kinase and Akt signaling in endothelial cells. J Biol Chem. 279:1304–1309. 2004. View Article : Google Scholar

33 

Martinelli R, Gegg M, Longbottom R, Adamson P, Turowski P and Greenwood J: ICAM-1-mediated endothelial nitric oxide synthase activation via calcium and AMP-activated protein kinase is required for transendothelial lymphocyte migration. Mol Biol Cell. 20:995–1005. 2009. View Article : Google Scholar :

34 

Park JJ, Seo SM, Kim EJ, Lee YJ, Ko YG, Ha J and Lee M: Berberine inhibits human colon cancer cell migration via AMP-activated protein kinase-mediated downregulation of integrin β1 signaling. Biochem Biophys Res Commun. 426:461–467. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Peyton KJ, Yu Y, Yates B, Shebib AR, Liu XM, Wang H and Durante W: Compound C inhibits vascular smooth muscle cell proliferation and migration in an AMP-activated protein kinase-independent fashion. J Pharmacol Exp Ther. 338:476–484. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Kim H, Lim J, Lee DY, Ryu J and Lim J: Kazinol C from Broussonetia kazinoki activates AMP-activated protein kinase to induce antitumorigenic effects in HT-29 colon cancer cells. Oncol Rep 33: 223-229, 2015.
APA
Kim, H., Lim, J., Lee, D.Y., Ryu, J., & Lim, J. (2015). Kazinol C from Broussonetia kazinoki activates AMP-activated protein kinase to induce antitumorigenic effects in HT-29 colon cancer cells. Oncology Reports, 33, 223-229. https://doi.org/10.3892/or.2014.3601
MLA
Kim, H., Lim, J., Lee, D. Y., Ryu, J., Lim, J."Kazinol C from Broussonetia kazinoki activates AMP-activated protein kinase to induce antitumorigenic effects in HT-29 colon cancer cells". Oncology Reports 33.1 (2015): 223-229.
Chicago
Kim, H., Lim, J., Lee, D. Y., Ryu, J., Lim, J."Kazinol C from Broussonetia kazinoki activates AMP-activated protein kinase to induce antitumorigenic effects in HT-29 colon cancer cells". Oncology Reports 33, no. 1 (2015): 223-229. https://doi.org/10.3892/or.2014.3601
Copy and paste a formatted citation
x
Spandidos Publications style
Kim H, Lim J, Lee DY, Ryu J and Lim J: Kazinol C from Broussonetia kazinoki activates AMP-activated protein kinase to induce antitumorigenic effects in HT-29 colon cancer cells. Oncol Rep 33: 223-229, 2015.
APA
Kim, H., Lim, J., Lee, D.Y., Ryu, J., & Lim, J. (2015). Kazinol C from Broussonetia kazinoki activates AMP-activated protein kinase to induce antitumorigenic effects in HT-29 colon cancer cells. Oncology Reports, 33, 223-229. https://doi.org/10.3892/or.2014.3601
MLA
Kim, H., Lim, J., Lee, D. Y., Ryu, J., Lim, J."Kazinol C from Broussonetia kazinoki activates AMP-activated protein kinase to induce antitumorigenic effects in HT-29 colon cancer cells". Oncology Reports 33.1 (2015): 223-229.
Chicago
Kim, H., Lim, J., Lee, D. Y., Ryu, J., Lim, J."Kazinol C from Broussonetia kazinoki activates AMP-activated protein kinase to induce antitumorigenic effects in HT-29 colon cancer cells". Oncology Reports 33, no. 1 (2015): 223-229. https://doi.org/10.3892/or.2014.3601
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