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Article

The V-val subtype Epstein-Barr virus nuclear antigen 1 promotes cell survival after serum withdrawal

  • Authors:
    • Mei Chao
    • Hsiao-Ning Wang
    • Yi Jin Lu
    • Yu-Sun Chang
    • Jau-Song Yu
  • View Affiliations / Copyright

    Affiliations: Division of Microbiology, Graduate Institute of Biomedical Sciences, Chang Gung University, Kwei-Shan, Tao-Yang 333, Taiwan R.O.C., Molecular Medicine Research Center, College of Medicine, Chang Gung University, Kwei-Shan, Tao-Yang 333, Taiwan R.O.C.
  • Pages: 958-966
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    Published online on: November 25, 2014
       https://doi.org/10.3892/or.2014.3625
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Abstract

Epstein-Barr virus (EBV) can establish latent infection and has been associated with various human cancers. Epstein-Barr nuclear antigen 1 (EBNA1) is the only viral protein that is expressed in all EBV-associated malignant tissues. The N- and C-terminal domains of EBNA1, which are connected by internal glycine/alanine-rich short repeat sequences of various sizes, show sequence divergence across EBV strains isolated from around the world. At least five subtypes have been described, according to the amino acid at residue 487: P-ala, P-thr, V-val, V-pro, and V-leu. Whether the variations of EBNA-1 contribute to the pathogenesis of EBV or simply reflect the geographical distribution of EBV remain to be investigated. Furthermore, the cell effects conferred by EBNA1 subtypes that differ from that of the B95.8 prototype, which belongs to the P-ala subtype, remain to be elucidated. In this study, PCR was amplified with the full-length V-val EBNA1 gene from the CG3 cell line, an EBV-carrying lymphoblastoid cell line derived from a Taiwanese chronic myeloid leukemia patient. Plasmids expressing His-tagged EBNA1 fusion proteins in E. coli were constructed and used to raise antibodies in rabbit. The V-val EBNA1 gene was then cloned into a eukaryotic expression vector and successfully expressed in the transfected cultured cells. Expression of V-val EBNA1 rendered 293 cells able to undergo serum‑independent cell proliferation, providing them with anti-apoptotic abilities, which are two characteristics of cancer cells. These data suggested that use of EBNA1 originally derived from tumor cells, rather than the more commonly utilized prototype, when investigating the potential role of EBNA1 in the oncogenesis of EBV-associated malignancies, is crucial.
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Spandidos Publications style
Chao M, Wang H, Lu YJ, Chang Y and Yu J: The V-val subtype Epstein-Barr virus nuclear antigen 1 promotes cell survival after serum withdrawal. Oncol Rep 33: 958-966, 2015.
APA
Chao, M., Wang, H., Lu, Y.J., Chang, Y., & Yu, J. (2015). The V-val subtype Epstein-Barr virus nuclear antigen 1 promotes cell survival after serum withdrawal. Oncology Reports, 33, 958-966. https://doi.org/10.3892/or.2014.3625
MLA
Chao, M., Wang, H., Lu, Y. J., Chang, Y., Yu, J."The V-val subtype Epstein-Barr virus nuclear antigen 1 promotes cell survival after serum withdrawal". Oncology Reports 33.2 (2015): 958-966.
Chicago
Chao, M., Wang, H., Lu, Y. J., Chang, Y., Yu, J."The V-val subtype Epstein-Barr virus nuclear antigen 1 promotes cell survival after serum withdrawal". Oncology Reports 33, no. 2 (2015): 958-966. https://doi.org/10.3892/or.2014.3625
Copy and paste a formatted citation
x
Spandidos Publications style
Chao M, Wang H, Lu YJ, Chang Y and Yu J: The V-val subtype Epstein-Barr virus nuclear antigen 1 promotes cell survival after serum withdrawal. Oncol Rep 33: 958-966, 2015.
APA
Chao, M., Wang, H., Lu, Y.J., Chang, Y., & Yu, J. (2015). The V-val subtype Epstein-Barr virus nuclear antigen 1 promotes cell survival after serum withdrawal. Oncology Reports, 33, 958-966. https://doi.org/10.3892/or.2014.3625
MLA
Chao, M., Wang, H., Lu, Y. J., Chang, Y., Yu, J."The V-val subtype Epstein-Barr virus nuclear antigen 1 promotes cell survival after serum withdrawal". Oncology Reports 33.2 (2015): 958-966.
Chicago
Chao, M., Wang, H., Lu, Y. J., Chang, Y., Yu, J."The V-val subtype Epstein-Barr virus nuclear antigen 1 promotes cell survival after serum withdrawal". Oncology Reports 33, no. 2 (2015): 958-966. https://doi.org/10.3892/or.2014.3625
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