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Article

Upregulated circulating miR-150 is associated with the risk of intrahepatic cholangiocarcinoma

  • Authors:
    • Shouli Wang
    • Jikai Yin
    • Tao Li
    • Lijuan Yuan
    • Dong Wang
    • Jiaxing He
    • Xilin Du
    • Jianguo Lu
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Tangdu Hospital of The Fourth Military Medical University, Shaanxi 710038, P.R. China, Department of General Surgery, General Hospital of Beijing Military Command, Beijing 100700, P.R. China
  • Pages: 819-825
    |
    Published online on: December 2, 2014
       https://doi.org/10.3892/or.2014.3641
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Abstract

During the last decade, microRNAs (miRNAs) have been identified as potential biomarkers and therapeutic targets for multiple malignancies; yet, few studies exist on intrahepatic cholangiocarcinoma (ICC). In the present study, a miRNA microarray was applied to determine the significant miRNAs involved in ICC. miR-150 was found to be significantly downregulated in ICC. We further enrolled 15 ICC patients who received radical resection to test these findings in plasma. Using quantitative reverse transcription-polymerase chain reaction (qRT-PCR), we examined and quantified the expression levels of miR-150 in tumor tissues, peritumoral noncancerous tissues and blood samples of 15 ICC patients. The diagnostic value of plasma miR-150 for differentiating patients with ICC from the age- and gender-matched controls was analyzed. For plasma samples, compared with normal controls, the level of miR-150 expression was found to be upregulated (P<0.010) in ICC patients. While differentiating ICC from normal controls, receiver operator curve (ROC) analysis of plasma miR-150 revealed the area under the curve (AUC) of 0.764 (P<0.010) with sensitivity of 80.6% and specificity of 58.1%. The diagnostic value of carbohydrate antigen 19-9 (CA19-9) and the combination of miR-150 and CA19-9 were also evaluated. We found that the combination of these two markers improved the power of screening ICC. Moreover, on the basis of the plasma miR-150 level, 15 ICC patients were divided into a low or high expression group. We found that plasma miR‑150 is a potential diagnostic biomarker for ICC.
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Copy and paste a formatted citation
Spandidos Publications style
Wang S, Yin J, Li T, Yuan L, Wang D, He J, Du X and Lu J: Upregulated circulating miR-150 is associated with the risk of intrahepatic cholangiocarcinoma. Oncol Rep 33: 819-825, 2015.
APA
Wang, S., Yin, J., Li, T., Yuan, L., Wang, D., He, J. ... Lu, J. (2015). Upregulated circulating miR-150 is associated with the risk of intrahepatic cholangiocarcinoma. Oncology Reports, 33, 819-825. https://doi.org/10.3892/or.2014.3641
MLA
Wang, S., Yin, J., Li, T., Yuan, L., Wang, D., He, J., Du, X., Lu, J."Upregulated circulating miR-150 is associated with the risk of intrahepatic cholangiocarcinoma". Oncology Reports 33.2 (2015): 819-825.
Chicago
Wang, S., Yin, J., Li, T., Yuan, L., Wang, D., He, J., Du, X., Lu, J."Upregulated circulating miR-150 is associated with the risk of intrahepatic cholangiocarcinoma". Oncology Reports 33, no. 2 (2015): 819-825. https://doi.org/10.3892/or.2014.3641
Copy and paste a formatted citation
x
Spandidos Publications style
Wang S, Yin J, Li T, Yuan L, Wang D, He J, Du X and Lu J: Upregulated circulating miR-150 is associated with the risk of intrahepatic cholangiocarcinoma. Oncol Rep 33: 819-825, 2015.
APA
Wang, S., Yin, J., Li, T., Yuan, L., Wang, D., He, J. ... Lu, J. (2015). Upregulated circulating miR-150 is associated with the risk of intrahepatic cholangiocarcinoma. Oncology Reports, 33, 819-825. https://doi.org/10.3892/or.2014.3641
MLA
Wang, S., Yin, J., Li, T., Yuan, L., Wang, D., He, J., Du, X., Lu, J."Upregulated circulating miR-150 is associated with the risk of intrahepatic cholangiocarcinoma". Oncology Reports 33.2 (2015): 819-825.
Chicago
Wang, S., Yin, J., Li, T., Yuan, L., Wang, D., He, J., Du, X., Lu, J."Upregulated circulating miR-150 is associated with the risk of intrahepatic cholangiocarcinoma". Oncology Reports 33, no. 2 (2015): 819-825. https://doi.org/10.3892/or.2014.3641
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