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Article

Overexpression of Lin28 inhibits the proliferation, migration and cell cycle progression and induces apoptosis of BGC-823 gastric cancer cells

  • Authors:
    • Hu Song
    • Wei Xu
    • Jun Song
    • Yong Liang
    • Wei Fu
    • Xiao‑Cheng Zhu
    • Chao Li
    • Jun-Sheng Peng
    • Jun-Nian Zheng

  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Surgery, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221002, P.R. China, Department of Gastrointestinal Surgery, The Sixth Affiliated Hospital (Gastrointestinal and Anal Hospital), Sun Yat-sen University, Guangzhou, Guangdong 510655, P.R. China, Jiangsu Province Tumor Biological Therapy Institute, Xuzhou, Jiangsu 221000, P.R. China
  • Pages: 997-1003
    |
    Published online on: December 15, 2014
       https://doi.org/10.3892/or.2014.3674
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Abstract

Lin28 plays important roles in the development, maintenance of pluripotency and progression of various types of cancers. Lin28 represses the biogenesis of let-7 microRNAs and is implicated in both development and tumorigenesis. Oncogenic regulation of let-7 microRNAs has been demonstrated in several human malignancies, yet their correlation with Lin28 has not yet been studied in gastric cancer. Therefore, in the present study, we explored the possible mechanisms involved in the effects by Lin28 on the proliferation, migration, cell cycle arrest and apoptosis in gastric cancer cells via alteration of let-7 miRNA. The expression levels of Lin28 and let-7 were detected by real-time PCR in gastric cancer cell lines in vitro. Lin28 was overexpressed in the BGC-823 cells via lentiviral transfection, and let-7 expression was assessed. Cell proliferation and migration capabilities were investigated by MTT and Transwell assays, while cell cycle distribution and the apoptosis rate were detected using flow cytometry. The expression of Lin28 was moderately expressed in the GES cells while underexpressed in the BGC-823, SGC-7901 and HGC-27 cells. Let-7a miRNA was highly expressed in the GES, BGC-823, SGC-7901 and HGC-27 cells. Overexpression of Lin28 was inversely correlated with the downregulated expression of let-7a, and markedly suppressed the proliferation, migration, cell cycle progression and induced apoptosis in the BGC-823 cells. These findings demonstrated that overexpression of Lin28 can suppress the biological behavior of gastric cancer in vitro, and let-7 miRNA may play an important role in the process. We suggest that Lin28 may be a candidate predictor or an anticancer therapeutic target for gastric cancer patients.
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Copy and paste a formatted citation
Spandidos Publications style
Song H, Xu W, Song J, Liang Y, Fu W, Zhu XC, Li C, Peng J and Zheng J: Overexpression of Lin28 inhibits the proliferation, migration and cell cycle progression and induces apoptosis of BGC-823 gastric cancer cells. Oncol Rep 33: 997-1003, 2015.
APA
Song, H., Xu, W., Song, J., Liang, Y., Fu, W., Zhu, X. ... Zheng, J. (2015). Overexpression of Lin28 inhibits the proliferation, migration and cell cycle progression and induces apoptosis of BGC-823 gastric cancer cells. Oncology Reports, 33, 997-1003. https://doi.org/10.3892/or.2014.3674
MLA
Song, H., Xu, W., Song, J., Liang, Y., Fu, W., Zhu, X., Li, C., Peng, J., Zheng, J."Overexpression of Lin28 inhibits the proliferation, migration and cell cycle progression and induces apoptosis of BGC-823 gastric cancer cells". Oncology Reports 33.2 (2015): 997-1003.
Chicago
Song, H., Xu, W., Song, J., Liang, Y., Fu, W., Zhu, X., Li, C., Peng, J., Zheng, J."Overexpression of Lin28 inhibits the proliferation, migration and cell cycle progression and induces apoptosis of BGC-823 gastric cancer cells". Oncology Reports 33, no. 2 (2015): 997-1003. https://doi.org/10.3892/or.2014.3674
Copy and paste a formatted citation
x
Spandidos Publications style
Song H, Xu W, Song J, Liang Y, Fu W, Zhu XC, Li C, Peng J and Zheng J: Overexpression of Lin28 inhibits the proliferation, migration and cell cycle progression and induces apoptosis of BGC-823 gastric cancer cells. Oncol Rep 33: 997-1003, 2015.
APA
Song, H., Xu, W., Song, J., Liang, Y., Fu, W., Zhu, X. ... Zheng, J. (2015). Overexpression of Lin28 inhibits the proliferation, migration and cell cycle progression and induces apoptosis of BGC-823 gastric cancer cells. Oncology Reports, 33, 997-1003. https://doi.org/10.3892/or.2014.3674
MLA
Song, H., Xu, W., Song, J., Liang, Y., Fu, W., Zhu, X., Li, C., Peng, J., Zheng, J."Overexpression of Lin28 inhibits the proliferation, migration and cell cycle progression and induces apoptosis of BGC-823 gastric cancer cells". Oncology Reports 33.2 (2015): 997-1003.
Chicago
Song, H., Xu, W., Song, J., Liang, Y., Fu, W., Zhu, X., Li, C., Peng, J., Zheng, J."Overexpression of Lin28 inhibits the proliferation, migration and cell cycle progression and induces apoptosis of BGC-823 gastric cancer cells". Oncology Reports 33, no. 2 (2015): 997-1003. https://doi.org/10.3892/or.2014.3674
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