Nucleosome-binding protein HMGN2 exhibits antitumor activity in human SaO2 and U2-OS osteosarcoma cell lines Corrigendum in /10.3892/or.2024.8827

Liang,Guojun; Xu,Enjie; Yang,Chaoqun; Zhang,Chenglin; Sheng,Xiaolong; Zhou,Xuhui

doi:10.3892/or.2014.3689

March-2015 Volume 33 Issue 3

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

March-2015 Volume 33 Issue 3

Full Size Image

Article

Nucleosome-binding protein HMGN2 exhibits antitumor activity in human SaO2 and U2-OS osteosarcoma cell lines

Corrigendum in: /10.3892/or.2024.8827

Authors:
- Guojun Liang
- Enjie Xu
- Chaoqun Yang
- Chenglin Zhang
- Xiaolong Sheng
- Xuhui Zhou
View Affiliations / Copyright

Affiliations: Department of Spine Surgery, Xinchang People's Hospital, Xinchang, Zhejiang 312500, P.R. China, Department of Orthopedic Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China
Pages: 1300-1306
|
Published online on: December 22, 2014

https://doi.org/10.3892/or.2014.3689
Expand metrics +

Abstract

High mobility group N (HMGNs) are members of the high mobility group protein family, and are involved in the development and progression of several tumors. HMGN1 and HMGN5 were previously shown to be associated with the bioactivities of osteosarcoma. However, the effects and molecular mechanisms of HMGN2 on osteosarcoma progression remain to be determined. In order to characterize the endogenous expression of HMGN2 in osteosarcoma cell lines, RT-PCR and western blot analysis were performed. Recombinant HMGN2 lentivirus was used to infect the osteosarcoma cell lines with relatively low HMGN2 expression to determine the functional relevance of HMGN2 overexpression in osteosarcoma cell growth and migration in vitro and in vivo, and to investigate the expression levels of Ki-67, PCNA, cyclin D1 and cyclin E. The results showed that osteosarcoma cell proliferation and migration were significantly reduced by HMGN2, as indicated by cell count and wound-healing assays. Cell apoptosis was markedly induced and HMGN2 increased the sensitivity to chemotherapy. When HMGN2 expression was enhanced, the expression of cyclin D1 and PCNA was downregulated in osteosarcoma cells. In addition, the tumor volumes in SaO2 and U2-OS subcutaneous nude mouse models treated with HMGN2 lentivirus were significantly decreased as compared to those of the GFP group. These results suggested that the enhanced expression of HMGN2 in osteosarcoma cells by HMGN2 lentivirus, exerts inhibitory effects on growth and migration of osteosarcoma cells.

View Figures

View References

1	Huang J, Ni J, Liu K, et al: HMGB1 promotes drug resistance in osteosarcoma. Cancer Res. 72:230–238. 2012. View Article : Google Scholar
2	Clark JC, Dass CR and Choong PF: A review of clinical and molecular prognostic factors in osteosarcoma. J Cancer Res Clin Oncol. 134:281–297. 2008. View Article : Google Scholar
3	Meyers PA, Schwartz CL, Krailo MD, et al: Osteosarcoma: the addition of muramyl tripeptide to chemotherapy improves overall survival - a report from the Children’s Oncology Group. J Clin Oncol. 26:633–638. 2008. View Article : Google Scholar : PubMed/NCBI
4	Lau CC, Harris CP, Lu XY, et al: Frequent amplification and rearrangement of chromosomal bands 6p12-p21 and 17p11.2 in osteosarcoma. Genes Chromosomes Cancer. 39:11–21. 2004. View Article : Google Scholar
5	Musselman CA and Kutateladze TG: Methyl fingerprinting of the nucleosome reveals the molecular mechanism of high-mobility group nucleosomal-2 (HMGN2) association. Proc Natl Acad Sci USA. 108:12189–12190. 2011. View Article : Google Scholar : PubMed/NCBI
6	Bustin M: Regulation of DNA-dependent activities by the functional motifs of the high-mobility-group chromosomal proteins. Mol Cell Biol. 19:5237–5246. 1999.PubMed/NCBI
7	Postnikov YV, Herrera JE, Hock R, Scheer U and Bustin M: Clusters of nucleosomes containing chromosomal protein HMG-17 in chromatin. J Mol Biol. 274:454–465. 1997. View Article : Google Scholar
8	Porkka K, Laakkonen P, Hoffman JA, Bernasconi M and Ruoslahti E: A fragment of the HMGN2 protein homes to the nuclei of tumor cells and tumor endothelial cells in vivo. Proc Natl Acad Sci USA. 99:7444–7449. 2002. View Article : Google Scholar : PubMed/NCBI
9	Srikantha T, Landsman D and Bustin M: Retropseudogenes for human chromosomal protein HMG-17. J Mol Biol. 197:405–413. 1987. View Article : Google Scholar : PubMed/NCBI
10	Spieker N, Beitsma M, van Sluis P, et al: An integrated 5-Mb physical, genetic, and radiation hybrid map of a 1p36.1 region implicated in neuroblastoma pathogenesis. Genes Chromosomes Cancer. 27:143–152. 2000. View Article : Google Scholar
11	Hu A, Dong X, Liu X, et al: Nucleosome-binding protein HMGN2 exhibits antitumor activity in oral squamous cell carcinoma. Oncol Lett. 7:115–120. 2014.
12	Liu X, Dong X, Zhang Y, et al: Inhibitory effects of high mobility group chromosomal protein N2 on human tongue carcinoma transplanted in nude mice. Hua Xi Kou Qiang Yi Xue Za Zhi. 32:5–8. 2014.(In Chinese). PubMed/NCBI
13	Evans DL, Bishop GR and Jaso-Friedmann L: Methods for cell cycle analysis and detection of apoptosis of teleost cells. Methods Cell Sci. 22:225–231. 2000. View Article : Google Scholar
14	Birger Y, Catez F, Furusawa T, et al: Increased tumorigenicity and sensitivity to ionizing radiation upon loss of chromosomal protein HMGN1. Cancer Res. 65:6711–6718. 2005. View Article : Google Scholar : PubMed/NCBI
15	Zhou X, Yuan B, Yuan W, Wang C, Gao R and Wang J: The expression and clinical significance of high mobility group nucleosome binding domain 5 in human osteosarcoma. Tumour Biol. 35:6539–6547. 2014. View Article : Google Scholar : PubMed/NCBI
16	Li DQ, Hou YF, Wu J, et al: Gene expression profile analysis of an isogenic tumour metastasis model reveals a functional role for oncogene AF1Q in breast cancer metastasis. Eur J Cancer. 42:3274–3286. 2006. View Article : Google Scholar : PubMed/NCBI
17	Huang C, Zhou LQ and Song G: Effect of nucleosomal binding protein 1 in androgen-independent prostatic carcinoma. Zhonghua Yi Xue Za Zhi. 88:657–660. 2008.(In Chinese). PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Nucleosome-binding protein HMGN2 exhibits antitumor activity in human SaO2 and U2-OS osteosarcoma cell lines

Corrigendum in: /10.3892/or.2024.8827

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Related Articles