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Article

Aged garlic extract inhibits 1,2-dimethylhydrazine-induced colon tumor development by suppressing cell proliferation

  • Authors:
    • Hiroshi Jikihara
    • Guangying Qi
    • Koichiro Nozoe
    • Mayumi Hirokawa
    • Hiromi Sato
    • Yuka Sugihara
    • Fumio Shimamoto
  • View Affiliations / Copyright

    Affiliations: Program in Biological System Sciences, Prefectural University of Hiroshima, Minami-ku, Hiroshima 734-8558, Japan, Department of Health Science, Faculty of Human Culture Science, Prefectural University of Hiroshima, Minami-ku, Hiroshima 734-8558, Japan
  • Pages: 1131-1140
    |
    Published online on: December 30, 2014
       https://doi.org/10.3892/or.2014.3705
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Abstract

Garlic and its constituents are reported to have a preventive effect against colorectal cancer in animal models. Aged garlic extract (AGE), which is produced by natural extraction from fresh garlic for more than 10 months in aqueous ethanol, also has reputed chemopreventive effects on colon carcinogenesis, but has never been studied for its effects on colon cancer development. We investigated the antitumor effects of AGE in rats with 1,2-dimethylhydrazine (DMH)-induced carcinogenesis, and the mechanism of AGE in human colon cancer cell proliferation. F344 rats randomly divided into three groups were administered DMH (20 mg/kg weight) subcutaneously once a week for 8 weeks in a basal diet. After the last injection, one group of rats was then moved onto a basal diet containing 3% wt/wt AGE, and rats were sacrificed at 8 or 31 weeks. The number of aberrant crypt foci (ACF), histological type of tumor and proliferative activity of the tumor lesions were analyzed by macroscopic, pathological and immunohistochemical methods. DLD-1 human colon cancer cells were utilized to investigate the effect of AGE on anti-cell proliferation. AGE decreased the number of ACF but had no effect on gross tumor pathology. AGE showed a lower number of adenoma and adenocarcinoma lesions by histological analysis. Immunohistochemical staining indicated that AGE suppressed the proliferative activity in adenoma and adenocarcinoma lesions, but showed no effect on normal colon mucosa. Moreover, we demonstrated that AGE delayed cell cycle progression by downregulating cyclin B1 and cdk1 expression via inactivation of NF-κB in the human colorectal cancer cells but did not induce apoptosis. These findings suggest that AGE has an antitumor effect through suppression of cell proliferation.
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1 

Fleischauer AT, Poole C and Arab L: Garlic consumption and cancer prevention: meta-analyses of colorectal and stomach cancers. Am J Clin Nutr. 72:1047–1052. 2000.PubMed/NCBI

2 

Gao CM, Takezaki T, Ding JH, Li MS and Tajima K: Protective effect of allium vegetables against both esophageal and stomach cancer: a simultaneous case-referent study of a high-epidemic area in Jiangsu Province, China. Jpn J Cancer Res. 90:614–621. 1999. View Article : Google Scholar : PubMed/NCBI

3 

Sumiyoshi H and Wargovich MJ: Chemoprevention of 1,2-dimethylhydrazine-induced colon cancer in mice by naturally occurring organosulfur compounds. Cancer Res. 15:5084–5087. 1990.

4 

Hatono S, Jimenez A and Wargovich MJ: Chemopreventive effect of S-allylcysteine and its relationship to the detoxification enzyme glutathione S-transferase. Carcinogenesis. 17:1041–1044. 1996. View Article : Google Scholar : PubMed/NCBI

5 

Wang X, Jiao F, Wang QW, et al: Aged black garlic extract induces inhibition of gastric cancer cell growth in vitro and in vivo. Mol Med Rep. 5:66–72. 2012.

6 

Xiao D, Pinto JT, Soh JW, Deguchi A, et al: Induction of apoptosis by the garlic-derived compound S-allylmercaptocysteine (SAMC) is associated with microtubule depolymerization and c-Jun NH(2)-terminal kinase 1 activation. Cancer Res. 63:6825–6837. 2003.PubMed/NCBI

7 

Knowles LM and Milner JA: Depressed p34 cdc2 kinase activity and G2/M phase arrest induced by diallyl disulfide in HCT-15 cells. Nutr Cancer. 30:169–174. 1998. View Article : Google Scholar

8 

Shirin H, Pinto JT, Kawabata Y, et al: Antiproliferative effects of S-allylmercaptocysteine on colon cancer cells when tested alone or in combination with sulindac sulfide. Cancer Res. 61:725–731. 2001.PubMed/NCBI

9 

Hosono T, Fukao T, Ogihara J, Ito Y, Shiba H, Seki T and Ariga T: Diallyl trisulfide suppresses the proliferation and induces apoptosis of human colon cancer cells through oxidative modification of beta-tubulin. J Biol Chem. 280:41487–41493. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Ban JO, Yuk DY, Woo KS, et al: Inhibition of cell growth and induction of apoptosis via inactivation of NF-kappaB by a sulfur-compound isolated from garlic in human colon cancer cells. J Pharmacol Sci. 104:374–383. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Sriram N, Kalayarasan S, Ashokkumar P, Sureshkumar A and Sudhandiran G: Diallyl sulfide induces apoptosis in Colo 320 DM human colon cancer cells: involvement of caspase-3, NF-kappaB, and ERK-2. Mol Cell Biochem. 311:157–165. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Liang D, Qin Y, Zhao W, et al: S-allylmercaptocysteine effectively inhibits the proliferation of colorectal cancer cells under in vitro and in vivo conditions. Cancer Lett. 310:69–76. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Dong M, Yang G, Liu H, Liu X, Lin S, Sun D and Wang Y: Aged black garlic extract inhibits HT29 colon cancer cell growth via the PI3K/Akt signaling pathway. Biomed Rep. 2:250–254. 2014.PubMed/NCBI

14 

Soni KB, Lahiri M, Chackradeo P, Bhide SV and Kuttan R: Protective effect of food additives on aflatoxin-induced mutagenicity and hepatocarcinogenicity. Cancer Lett. 115:129–133. 1997. View Article : Google Scholar : PubMed/NCBI

15 

Knasmüller S, de Martin R, Domjan G and Szakmary A: Studies on the antimutagenic activities of garlic extract. Environ Mol Mutagen. 13:357–365. 1989. View Article : Google Scholar : PubMed/NCBI

16 

Amagase H and Milner JA: Impact of various sources of garlic and their constituents on 7,12-dimethylbenz[a]anthracene binding to mammary cell DNA. Carcinogenesis. 14:1627–1631. 1993. View Article : Google Scholar : PubMed/NCBI

17 

Sparnins VL, Barany G and Wattenberg LW: Effects of organosulfur compounds from garlic and onions on benzo[a] pyrene-induced neoplasia and glutathione S-transferase activity in the mouse. Carcinogenesis. 9:131–134. 1988. View Article : Google Scholar : PubMed/NCBI

18 

Tadi PP, Tee RW and Lau BH: Organosulfur compounds of garlic modulate mutagenesis, metabolism, and DNA binding of aflatoxin B1. Nutr Cancer. 15:87–95. 1991. View Article : Google Scholar : PubMed/NCBI

19 

Perše M and Cerar A: Morphological and molecular alterations in 1,2 dimethylhydrazine and azoxymethane induced colon carcinogenesis in rats. J Biomed Biotechnol. 2011:1–14. 2011. View Article : Google Scholar

20 

Tammariello AE and Milner JA: Mouse models for unraveling the importance of diet in colon cancer prevention. J Nutr Biochem. 21:77–88. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Chen J and Huang XF: The signal pathways in azoxymethane-induced colon cancer and preventive implications. Cancer Biol Ther. 8:1313–1317. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Takahashi M and Wakabayashi K: Gene mutations and altered gene expression in azoxymethane-induced colon carcinogenesis in rodents. Cancer Sci. 95:475–480. 2004. View Article : Google Scholar : PubMed/NCBI

23 

Lijinsky W, Saavedra JE and Reuber MD: Organ-specific carcinogenesis in rats by methyl- and ethylazoxyalkanes. Cancer Res. 45:76–79. 1985.PubMed/NCBI

24 

Tanaka S, Haruma K, Kunihiro M, et al: Effects of aged garlic extract (AGE) on colorectal adenomas: a double-blinded study. Hiroshima J Med Sci. 53:39–45. 2004.

25 

Matsuura N, Miyamae Y, Yamane K, et al: Aged garlic extract inhibits angiogenesis and proliferation of colorectal carcinoma cells. J Nutr. 136(Suppl 3): 842–846. 2006.

26 

Kyo E, Uda N, Kasuga S and Itakura Y: Immunomodulatory effects of aged garlic extract. J Nutr. 131:S1075–S1079. 2006.

27 

Morihara N, Sumioka I, Moriguchi T, Uda N and Kyo E: Aged garlic extract enhances production of nitric oxide. Life Sci. 71:509–517. 2002. View Article : Google Scholar : PubMed/NCBI

28 

Morihara N, Ushijima M, Kashimoto N, et al: Aged garlic extract ameliorates physical fatigue. Biol Pharm Bull. 29:962–966. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Katsuki T, Hirata K, Ishikawa H, Matsuura N, Sumi S and Itoh H: Aged garlic extract has chemopreventative effects on 1,2-dimethylhydrazine-induced colon tumors in rats. J Nutr. 136(Suppl 3): 847–851. 2006.

30 

McLellan EA, Medline A and Bird RP: Sequential analyses of the growth and morphological characteristics of aberrant crypt foci: putative preneoplastic lesions. Cancer Res. 51:5270–5274. 1991.PubMed/NCBI

31 

Pindborg JJ, Reichart PA, Smith CJ and Waal I: Histological typing of cancer and precancer of the oral mucosa. 2nd edition. WHO; Geneva: pp. 21–31. 1997

32 

Ferreira CG, Epping M, Kruyt FA and Giaccone G: Apoptosis: target of cancer therapy. Clin Cancer Res 2002. 8:2024–2034. 2002.

33 

Alnemri ES, Livingston DJ, Nicholson DW, Salvesen G, Thornberry NA, Wong WW and Yuan J: Human ICE/CED-3 protease nomenclature. Cell. 87:1711996. View Article : Google Scholar : PubMed/NCBI

34 

Muschel RJ, Zhang HB, Iliakis G and McKenna WG: Cyclin B expression in HeLa cells during the G2 block induced by ionizing radiation. Cancer Res. 51:5113–5117. 1991.PubMed/NCBI

35 

Metting NF and Little JB: Transient failure to dephosphorylate the cdc2-cyclin B1 complex accompanies radiation-induced G2-phase arrest in HeLa cells. Radiat Res. 143:286–292. 1995. View Article : Google Scholar : PubMed/NCBI

36 

Kao GD, McKenna WG, Maity A, Blank K and Muschel RJ: Cyclin B1 availability is a rate-limiting component of the radiation-induced G2 delay in HeLa cells. Cancer Res. 57:753–758. 1997.PubMed/NCBI

37 

Azzam EI, de Toledo SM, Gooding T and Little JB: Intercellular communication is involved in the bystander regulation of gene expression in human cells exposed to very low fluences of alpha particles. Radiat Res. 150:497–504. 1998. View Article : Google Scholar : PubMed/NCBI

38 

Guo G, Yan-Sanders Y, Lyn-Cook BD, et al: Manganese superoxide dismutase-mediated gene expression in radiation-induced adaptive responses. Mol Cell Biol. 23:2362–2378. 2003. View Article : Google Scholar : PubMed/NCBI

39 

Ozeki M, Tamae D, Hou DX, Wang T, Lebon T, Spitz DR and Li JJ: Response of cyclin B1 to ionizing radiation: regulation by NF-kappaB and mitochondrial antioxidant enzyme MnSOD. Anticancer Res. 24:2657–2663. 2004.PubMed/NCBI

40 

Herman-Antosiewicz A, Powolny AA and Singh SV: Molecular targets of cancer chemoprevention by garlic-derived organo-sulfides. Acta Pharmacol Sin. 28:1355–1364. 2007. View Article : Google Scholar : PubMed/NCBI

41 

Pretlow TP1, Cheyer C and O’Riordan MA: Aberrant crypt foci and colon tumors in F344 rats have similar increases in proliferative activity. Int J Cancer. 56:599–602. 1994. View Article : Google Scholar : PubMed/NCBI

42 

Hur K, Kim JR, Yoon BI, Lee JK, Choi JH, Oh GT and Kim DY: Overexpression of cyclin D1 and cyclin E in 1,2-dimethylhydrazine dihydrochloride-induced rat colon carcinogenesis. J Vet Sci. 1:121–126. 2000.

43 

Janakiram NB, Mohammed A, Qian L, Choi CI, Steele VE and Rao CV: Chemopreventive effects of RXR-selective rexinoid bexarotene on intestinal neoplasia of Apc(Min/+) mice. Neoplasia. 14:159–168. 2012.PubMed/NCBI

44 

Powolny AA and Singh SV: Multitargeted prevention and therapy of cancer by diallyl trisulfide and related Allium vegetable-derived organosulfur compounds. Cancer Lett. 269:305–314. 2008. View Article : Google Scholar : PubMed/NCBI

45 

Pai MH, Kuo YH, Chiang EP and Tang FY: S-Allylcysteine inhibits tumour progression and the epithelial-mesenchymal transition in a mouse xenograft model of oral cancer. Br J Nutr. 14:28–38. 2012. View Article : Google Scholar

46 

Xiao D, Vogel V and Singh SV: Benzyl isothiocyanate-induced apoptosis in human breast cancer cells is initiated by reactive oxygen species and regulated by Bax and Bak. Mol Cancer Ther. 5:2931–2945. 2006. View Article : Google Scholar : PubMed/NCBI

47 

Ryu DS, Kim SH and Lee DS: Anti-proliferative effect of polysaccharides from Salicornia herbacea on induction of G2/M arrest and apoptosis in human colon cancer cells. J Microbiol Biotechnol. 19:1482–1489. 2009. View Article : Google Scholar : PubMed/NCBI

48 

Huang S, Pettaway CA, Uehara H, Bucana CD and Fidler IJ: Blockade of NF-kappaB activity in human prostate cancer cells is associated with suppression of angiogenesis, invasion, and metastasis. Oncogene. 20:4188–4197. 2001. View Article : Google Scholar : PubMed/NCBI

49 

Lee SH, Lee CW, Lee JW, et al: Induction of apoptotic cell death by 2′-hydroxycinnamaldehyde is involved with ERK-dependent inactivation of NF-kappaB in TNF-alpha-treated SW620 colon cancer cells. Biochem Pharmacol. 70:1147–1157. 2005. View Article : Google Scholar : PubMed/NCBI

50 

Saccani A, Schioppa T, Porta C, et al: p50 nuclear factor-kappaB overexpression in tumor-associated macrophages inhibits M1 inflammatory responses and antitumor resistance. Cancer Res. 66:11432–11440. 2006. View Article : Google Scholar : PubMed/NCBI

51 

Tang X, Liu D, Shishodia S, et al: Nuclear factor-kappaB (NF-kappaB) is frequently expressed in lung cancer and preneoplastic lesions. Cancer. 107:2637–2646. 2006. View Article : Google Scholar : PubMed/NCBI

52 

Hardwick JC, van den Brink GR, Offerhaus GJ, van Deventer SJ and Peppelenbosch MP: NF-kappaB, p38 MAPK and JNK are highly expressed and active in the stroma of human colonic adenomatous polyps. Oncogene. 20:819–827. 2001. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Jikihara H, Qi G, Nozoe K, Hirokawa M, Sato H, Sugihara Y and Shimamoto F: Aged garlic extract inhibits 1,2-dimethylhydrazine-induced colon tumor development by suppressing cell proliferation. Oncol Rep 33: 1131-1140, 2015.
APA
Jikihara, H., Qi, G., Nozoe, K., Hirokawa, M., Sato, H., Sugihara, Y., & Shimamoto, F. (2015). Aged garlic extract inhibits 1,2-dimethylhydrazine-induced colon tumor development by suppressing cell proliferation. Oncology Reports, 33, 1131-1140. https://doi.org/10.3892/or.2014.3705
MLA
Jikihara, H., Qi, G., Nozoe, K., Hirokawa, M., Sato, H., Sugihara, Y., Shimamoto, F."Aged garlic extract inhibits 1,2-dimethylhydrazine-induced colon tumor development by suppressing cell proliferation". Oncology Reports 33.3 (2015): 1131-1140.
Chicago
Jikihara, H., Qi, G., Nozoe, K., Hirokawa, M., Sato, H., Sugihara, Y., Shimamoto, F."Aged garlic extract inhibits 1,2-dimethylhydrazine-induced colon tumor development by suppressing cell proliferation". Oncology Reports 33, no. 3 (2015): 1131-1140. https://doi.org/10.3892/or.2014.3705
Copy and paste a formatted citation
x
Spandidos Publications style
Jikihara H, Qi G, Nozoe K, Hirokawa M, Sato H, Sugihara Y and Shimamoto F: Aged garlic extract inhibits 1,2-dimethylhydrazine-induced colon tumor development by suppressing cell proliferation. Oncol Rep 33: 1131-1140, 2015.
APA
Jikihara, H., Qi, G., Nozoe, K., Hirokawa, M., Sato, H., Sugihara, Y., & Shimamoto, F. (2015). Aged garlic extract inhibits 1,2-dimethylhydrazine-induced colon tumor development by suppressing cell proliferation. Oncology Reports, 33, 1131-1140. https://doi.org/10.3892/or.2014.3705
MLA
Jikihara, H., Qi, G., Nozoe, K., Hirokawa, M., Sato, H., Sugihara, Y., Shimamoto, F."Aged garlic extract inhibits 1,2-dimethylhydrazine-induced colon tumor development by suppressing cell proliferation". Oncology Reports 33.3 (2015): 1131-1140.
Chicago
Jikihara, H., Qi, G., Nozoe, K., Hirokawa, M., Sato, H., Sugihara, Y., Shimamoto, F."Aged garlic extract inhibits 1,2-dimethylhydrazine-induced colon tumor development by suppressing cell proliferation". Oncology Reports 33, no. 3 (2015): 1131-1140. https://doi.org/10.3892/or.2014.3705
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