Kinesin superfamily protein 17 contributes to the development of bone cancer pain by participating in NR2B transport in the spinal cord of mice

  • Authors:
    • Ming Liu
    • Yue Liu
    • Bailing Hou
    • Dan Bu
    • Linyu Shi
    • Xiaoping Gu
    • Zhengliang Ma
  • View Affiliations

  • Published online on: January 7, 2015     https://doi.org/10.3892/or.2015.3706
  • Pages: 1365-1371
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Abstract

Τreatment of bone cancer pain remains a challenge, while the mechanisms causing the pain remain elusive. We demonstrated that the expression of the N‑methyl‑D‑aspartate (NMDA) receptor NR2B subunit was upregulated in mice with bone cancer pain. Kinesin superfamily protein 17 (KIF17), a recently characterized member of the kinesin superfamily proteins, has been demonstrated to transport and deliver the NR2B subunit to dendrites in mammalian neurons. In the present study, we induced bone cancer pain via femur bone cavity osteosarcoma NCTC 2472 tumor cell implantation (TCI) in mice. The results showed that TCI in mice increased the number of spontaneous flinches, mechanical allodynia events, expression of spinal KIF17 and NR2B subunits. Intrathecal administration of KIF17 antisense oligodeoxynucleotide (ODN) attenuated the behavioral signs of bone cancer pain and suppressed the increased expression of NR2B induced by TCI. In addition, KIF17 binds to a protein complex that contains mLin‑10 to transport NR2B, and we determined that the increase of mLin‑10 was suppressed following admini­stration. Thus, these findings suggested that KIF17 contributed to the development of bone cancer pain in the spinal cord through NR2B transport and that mLin‑10 may also play a role in pain development.
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March-2015
Volume 33 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Liu M, Liu Y, Hou B, Bu D, Shi L, Gu X and Ma Z: Kinesin superfamily protein 17 contributes to the development of bone cancer pain by participating in NR2B transport in the spinal cord of mice. Oncol Rep 33: 1365-1371, 2015
APA
Liu, M., Liu, Y., Hou, B., Bu, D., Shi, L., Gu, X., & Ma, Z. (2015). Kinesin superfamily protein 17 contributes to the development of bone cancer pain by participating in NR2B transport in the spinal cord of mice. Oncology Reports, 33, 1365-1371. https://doi.org/10.3892/or.2015.3706
MLA
Liu, M., Liu, Y., Hou, B., Bu, D., Shi, L., Gu, X., Ma, Z."Kinesin superfamily protein 17 contributes to the development of bone cancer pain by participating in NR2B transport in the spinal cord of mice". Oncology Reports 33.3 (2015): 1365-1371.
Chicago
Liu, M., Liu, Y., Hou, B., Bu, D., Shi, L., Gu, X., Ma, Z."Kinesin superfamily protein 17 contributes to the development of bone cancer pain by participating in NR2B transport in the spinal cord of mice". Oncology Reports 33, no. 3 (2015): 1365-1371. https://doi.org/10.3892/or.2015.3706