The ALDH1+ subpopulation of the human NMFH‑1 cell line exhibits cancer stem-like characteristics

  • Authors:
    • Dejian Li
    • Tao Zhang
    • Wenguang Gu
    • Peng Li
    • Xiangyang Cheng
    • Tiejun Tong
    • Wenbo Wang
  • View Affiliations

  • Published online on: March 9, 2015     https://doi.org/10.3892/or.2015.3842
  • Pages: 2291-2298
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Abstract

Cancer stem cells (CSCs) have been reported in many tissues. However, CSCs have yet to be identified in a human malignant fibrous histiocytoma (MFH) cell line. Elevated aldehyde dehydrogenase 1 (ALDH1) has been proposed as a stem cell marker for isolating CSCs from cancer. The aim of the present study was to identify a population with elevated ALDH in the human NMFH-1 cell line. ALDH+ and ALDH- cell populations were isolated and compared for CSC characteristics. ALDH enzymatic activity was used as a marker to identify the cells in the NMFH-1 line. Self-renewal, differentiation capacity, and tumorigenicity of the NMFH-1 ALDH+ cell population were then examined using a spheroid formation assay and xenograft model in nude mice. Chemoresistance levels, ABCG2 drug transport gene expression, and stem cell‑associated gene expression were compared in these NMFH-1 populations. The ALDH+ population was better able to form spheres in anchorage-independent serum‑starved conditions. Furthermore, the mRNA expression of key stem cell-related genes was enhanced in these cells. Increased expression of the drug transporter gene, ABCG2, was detected. Compared with ALDH-, the ALDH+ subpopulation had higher levels of chemoresistance to doxorubicin (DXR) and cisplatin (CDDP). Additionally, the ALDH+ cells more efficiently formed tumors when implanted into BALB/c nude mice. ALDH1 may therefore be used as a marker for the isolation of cells that exhibit several characteristics of CSCs from the NMFH-1 cell line. This finding may lead to the development of novel therapies to specifically kill ALDH1+ subpopulations (CSCs).
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May-2015
Volume 33 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Li D, Zhang T, Gu W, Li P, Cheng X, Tong T and Wang W: The ALDH1+ subpopulation of the human NMFH‑1 cell line exhibits cancer stem-like characteristics. Oncol Rep 33: 2291-2298, 2015
APA
Li, D., Zhang, T., Gu, W., Li, P., Cheng, X., Tong, T., & Wang, W. (2015). The ALDH1+ subpopulation of the human NMFH‑1 cell line exhibits cancer stem-like characteristics. Oncology Reports, 33, 2291-2298. https://doi.org/10.3892/or.2015.3842
MLA
Li, D., Zhang, T., Gu, W., Li, P., Cheng, X., Tong, T., Wang, W."The ALDH1+ subpopulation of the human NMFH‑1 cell line exhibits cancer stem-like characteristics". Oncology Reports 33.5 (2015): 2291-2298.
Chicago
Li, D., Zhang, T., Gu, W., Li, P., Cheng, X., Tong, T., Wang, W."The ALDH1+ subpopulation of the human NMFH‑1 cell line exhibits cancer stem-like characteristics". Oncology Reports 33, no. 5 (2015): 2291-2298. https://doi.org/10.3892/or.2015.3842