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Article

miR-203 inhibits arecoline-induced epithelial‑mesenchymal transition by regulating secreted frizzled-related protein 4 and transmembrane-4 L six family member 1 in oral submucous fibrosis

  • Authors:
    • Lian Zheng
    • Xinchun Jian
    • Feng Guo
    • Ning Li
    • Canhua Jiang
    • Ping Yin
    • An-Jie Min
    • Long Huang
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China
  • Pages: 2753-2760
    |
    Published online on: April 9, 2015
       https://doi.org/10.3892/or.2015.3909
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Abstract

Oral submucous fibrosis (OSF) is a potentially malignant disease predominantly found in Asian people. The areca nut has been implicated in this disease. Arecoline, one of the areca alkaloids, induces epithelial-mesenchymal transition (EMT)-related factors in primary human buccal mucosal fibroblasts. Yet, the mechanisms of the underlying arecoline-induced EMT in OSF remain unknown. In the present study, we aimed to investigate the role of microRNAs (miRNAs) in arecoline-induced EMT in HaCaT cells. We found that miR-203 was significantly downregulated in OSF tissues compared to that in normal buccal mucosa tissues, and that miR-203 negatively regulated secreted frizzled-related protein 4 (SFRP4) and positively regulated transmembrane-4 L six family member 1 (TM4SF1). We observed that upregulation of miR-203 significantly decreased the cell proliferation of HaCaT cells, and significantly upregulated the expression of cytokeratin 19 (CK19) and E-cadherin proteins, whereas it significantly downregulated the expression of N-cadherin and vimentin compared to these levels in the vehicle control cells. Thus, we provide evidence to illustrate that miR-203 plays a role in the pathogenesis of OSF, which may be a target for OSF management.
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Copy and paste a formatted citation
Spandidos Publications style
Zheng L, Jian X, Guo F, Li N, Jiang C, Yin P, Min A and Huang L: miR-203 inhibits arecoline-induced epithelial‑mesenchymal transition by regulating secreted frizzled-related protein 4 and transmembrane-4 L six family member 1 in oral submucous fibrosis. Oncol Rep 33: 2753-2760, 2015.
APA
Zheng, L., Jian, X., Guo, F., Li, N., Jiang, C., Yin, P. ... Huang, L. (2015). miR-203 inhibits arecoline-induced epithelial‑mesenchymal transition by regulating secreted frizzled-related protein 4 and transmembrane-4 L six family member 1 in oral submucous fibrosis. Oncology Reports, 33, 2753-2760. https://doi.org/10.3892/or.2015.3909
MLA
Zheng, L., Jian, X., Guo, F., Li, N., Jiang, C., Yin, P., Min, A., Huang, L."miR-203 inhibits arecoline-induced epithelial‑mesenchymal transition by regulating secreted frizzled-related protein 4 and transmembrane-4 L six family member 1 in oral submucous fibrosis". Oncology Reports 33.6 (2015): 2753-2760.
Chicago
Zheng, L., Jian, X., Guo, F., Li, N., Jiang, C., Yin, P., Min, A., Huang, L."miR-203 inhibits arecoline-induced epithelial‑mesenchymal transition by regulating secreted frizzled-related protein 4 and transmembrane-4 L six family member 1 in oral submucous fibrosis". Oncology Reports 33, no. 6 (2015): 2753-2760. https://doi.org/10.3892/or.2015.3909
Copy and paste a formatted citation
x
Spandidos Publications style
Zheng L, Jian X, Guo F, Li N, Jiang C, Yin P, Min A and Huang L: miR-203 inhibits arecoline-induced epithelial‑mesenchymal transition by regulating secreted frizzled-related protein 4 and transmembrane-4 L six family member 1 in oral submucous fibrosis. Oncol Rep 33: 2753-2760, 2015.
APA
Zheng, L., Jian, X., Guo, F., Li, N., Jiang, C., Yin, P. ... Huang, L. (2015). miR-203 inhibits arecoline-induced epithelial‑mesenchymal transition by regulating secreted frizzled-related protein 4 and transmembrane-4 L six family member 1 in oral submucous fibrosis. Oncology Reports, 33, 2753-2760. https://doi.org/10.3892/or.2015.3909
MLA
Zheng, L., Jian, X., Guo, F., Li, N., Jiang, C., Yin, P., Min, A., Huang, L."miR-203 inhibits arecoline-induced epithelial‑mesenchymal transition by regulating secreted frizzled-related protein 4 and transmembrane-4 L six family member 1 in oral submucous fibrosis". Oncology Reports 33.6 (2015): 2753-2760.
Chicago
Zheng, L., Jian, X., Guo, F., Li, N., Jiang, C., Yin, P., Min, A., Huang, L."miR-203 inhibits arecoline-induced epithelial‑mesenchymal transition by regulating secreted frizzled-related protein 4 and transmembrane-4 L six family member 1 in oral submucous fibrosis". Oncology Reports 33, no. 6 (2015): 2753-2760. https://doi.org/10.3892/or.2015.3909
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