MicroRNA-370 inhibits the progression of non-small cell lung cancer by downregulating oncogene TRAF4

  • Authors:
    • Tianjun Chen
    • Fei Gao
    • Sifang Feng
    • Tian Yang
    • Mingwei Chen
  • View Affiliations

  • Published online on: May 13, 2015     https://doi.org/10.3892/or.2015.3978
  • Pages: 461-468
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Abstract

Lung cancer is the leading cause of cancer-related deaths, of which most can be attributed to non-small cell lung cancer (NSCLC). microRNAs (miRNAs) are a group of small non-coding RNAs that focus on post-transcriptional modification. The present study aimed to investigate the role and function of microRNA-370 (miR-370) in NSCLC and explore the underlying functional mechanisms. We found that miR-370 was significantly downregulated in the tumor tissues of NSCLC patients as well as in NSCLC cell lines. Overexpression of miR-370 by infection of recombinant lentivirus markedly inhibited cell proliferation and promoted cell apoptosis of NSCLC cells. In addition, in vivo tumor formation of NSCLC cells was decreased by miR-370 overexpression. Through bioinformatic analysis, we found that tumor necrosis factor receptor-associated factor 4 (TRAF4), an oncogene as previously reported, was predicted as a putative target gene of miR-370. The direct targeting relationship between miR-370 and the 3'-untranslated region was validated by dual-luciferase reporter assay. Furthermore, overexpression of miR-370 downregulated the protein expression of TRAF4 in the NSCLC cells. Moreover, the growth inhibitory effect of miR-370 overexpression on NSCLC cells was abrogated by TRAF4 overexpression. In conclusion, our results suggest that miR-370 plays an important role in NSCLC by regulating TRAF4 and may be a potential target for the treatment of NSCLC.
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July-2015
Volume 34 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chen T, Gao F, Feng S, Yang T and Chen M: MicroRNA-370 inhibits the progression of non-small cell lung cancer by downregulating oncogene TRAF4. Oncol Rep 34: 461-468, 2015
APA
Chen, T., Gao, F., Feng, S., Yang, T., & Chen, M. (2015). MicroRNA-370 inhibits the progression of non-small cell lung cancer by downregulating oncogene TRAF4. Oncology Reports, 34, 461-468. https://doi.org/10.3892/or.2015.3978
MLA
Chen, T., Gao, F., Feng, S., Yang, T., Chen, M."MicroRNA-370 inhibits the progression of non-small cell lung cancer by downregulating oncogene TRAF4". Oncology Reports 34.1 (2015): 461-468.
Chicago
Chen, T., Gao, F., Feng, S., Yang, T., Chen, M."MicroRNA-370 inhibits the progression of non-small cell lung cancer by downregulating oncogene TRAF4". Oncology Reports 34, no. 1 (2015): 461-468. https://doi.org/10.3892/or.2015.3978