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Article

Recombinant hIFN-α2b-BCG inhibits tumor growth in a mouse model of bladder cancer

  • Authors:
    • Erlin Sun
    • Xiaodong Fan
    • Lining Wang
    • Mingde Lei
    • Xiaodong Zhou
    • Chunyu Liu
    • Bingxin Lu
    • Xuewu Nian
    • Yan Sun
    • Ruifa Han
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, Tianjin 300211, P.R. China, Department of Gynaecology, Tianjin City Hospital for Gynaecology and Obstetrics, Tianjin 300100, P.R. China
  • Pages: 183-194
    |
    Published online on: May 18, 2015
       https://doi.org/10.3892/or.2015.3985
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Abstract

Bacillus Calmette-Guérin (BCG) reduces the recurrence and progression of non-muscle invasive bladder cancer. The present study aimed to investigate the impact of a recombinant hIFN-α2b-secreting BCG (rBCG) on the mouse bladder MB49 cell line and an orthotopic mouse model of bladder cancer. MB49 cells were cultivated in the presence or absence of rBCG, BCG or BCG+hIFN-α2b. Cellular morphology and viability were assessed by microscopy and CCK-8 assay, respectively. Apoptosis was assessed by acridine orange, Hoechst 33258 staining and flow cytometry. MHC-I expression was assessed by flow cytometry. MB49 cells were transplanted into the bladders of C57BL/6 mice administered BCG, rBCG or BCG+hIFN-α2b. Local tissue Fas expression and T cell subsets were assessed by immunohistochemistry. Peripheral blood TNF-α and IL-12 levels were measured by ELISA, and circulating T lymphocyte subsets by flow cytometry. BCG, rBCG and BCG+hIFN-α2b increased the distortion and death of MB49 cells, yet rBCG reduced the proliferation and enhanced apoptosis most substantially. Apoptosis was increased after a 24-h co-culture with rBCG or BCG+hIFN-α2b. Mice administered rBCG survived longer than mice administered BCG (p<0.001), yet this result was not significantly different from mice administered BCG+hIFN-α2b. The average bladder weight was reduced by administration of rBCG (p<0.001). Fas expression and peripheral blood mTNF-α and mIL-12, cell counts of polymorphonuclear leukocytes, monocytes, T lymphocytes and CD4+/CD8+ ratios were significantly increased by all BCG treatments (p≤0.05), yet monocyte and T lymphocyte counts were higher in mice administered rBCG than in mice treated with BCG or BCG+hIFN-α2b (p=0.000). These results indicate that in an orthotopic murine bladder cancer model rBCG possesses superior antitumor activity to BCG+hIFN-α2b.
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Copy and paste a formatted citation
Spandidos Publications style
Sun E, Fan X, Wang L, Lei M, Zhou X, Liu C, Lu B, Nian X, Sun Y, Han R, Han R, et al: Recombinant hIFN-α2b-BCG inhibits tumor growth in a mouse model of bladder cancer. Oncol Rep 34: 183-194, 2015.
APA
Sun, E., Fan, X., Wang, L., Lei, M., Zhou, X., Liu, C. ... Han, R. (2015). Recombinant hIFN-α2b-BCG inhibits tumor growth in a mouse model of bladder cancer. Oncology Reports, 34, 183-194. https://doi.org/10.3892/or.2015.3985
MLA
Sun, E., Fan, X., Wang, L., Lei, M., Zhou, X., Liu, C., Lu, B., Nian, X., Sun, Y., Han, R."Recombinant hIFN-α2b-BCG inhibits tumor growth in a mouse model of bladder cancer". Oncology Reports 34.1 (2015): 183-194.
Chicago
Sun, E., Fan, X., Wang, L., Lei, M., Zhou, X., Liu, C., Lu, B., Nian, X., Sun, Y., Han, R."Recombinant hIFN-α2b-BCG inhibits tumor growth in a mouse model of bladder cancer". Oncology Reports 34, no. 1 (2015): 183-194. https://doi.org/10.3892/or.2015.3985
Copy and paste a formatted citation
x
Spandidos Publications style
Sun E, Fan X, Wang L, Lei M, Zhou X, Liu C, Lu B, Nian X, Sun Y, Han R, Han R, et al: Recombinant hIFN-α2b-BCG inhibits tumor growth in a mouse model of bladder cancer. Oncol Rep 34: 183-194, 2015.
APA
Sun, E., Fan, X., Wang, L., Lei, M., Zhou, X., Liu, C. ... Han, R. (2015). Recombinant hIFN-α2b-BCG inhibits tumor growth in a mouse model of bladder cancer. Oncology Reports, 34, 183-194. https://doi.org/10.3892/or.2015.3985
MLA
Sun, E., Fan, X., Wang, L., Lei, M., Zhou, X., Liu, C., Lu, B., Nian, X., Sun, Y., Han, R."Recombinant hIFN-α2b-BCG inhibits tumor growth in a mouse model of bladder cancer". Oncology Reports 34.1 (2015): 183-194.
Chicago
Sun, E., Fan, X., Wang, L., Lei, M., Zhou, X., Liu, C., Lu, B., Nian, X., Sun, Y., Han, R."Recombinant hIFN-α2b-BCG inhibits tumor growth in a mouse model of bladder cancer". Oncology Reports 34, no. 1 (2015): 183-194. https://doi.org/10.3892/or.2015.3985
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