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Article

USP39 promotes the growth of human hepatocellular carcinoma in vitro and in vivo

  • Authors:
    • Xianwen Yuan
    • Xitai Sun
    • Xiaolei Shi
    • Chunping Jiang
    • Decai Yu
    • Weiwei Zhang
    • Wenxian Guan
    • Jianxin Zhou
    • Yafu Wu
    • Yudong Qiu
    • Yitao Ding
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China, Department of General Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China
  • Pages: 823-832
    |
    Published online on: June 15, 2015
       https://doi.org/10.3892/or.2015.4065
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Abstract

Ubiquitin specific protease 39 (USP39) plays an important role in mRNA splicing. In the present study, we investigated the role of USP39 in regulating the growth of hepatocellular carcinoma (HCC). We detected USP39 expression in more than 100 HCC clinical samples. The USP39 expression was significantly higher in the tumor tissues compared to the adjacent normal tissues, and was strongly associated with the pathological grade of HCC. USP39 knockdown inhibited cell proliferation and colony formation in vitro in the HepG2 cells, while upregulation of USP39 promoted tumor cell growth. FCM assay showed that USP39 knockdown led to G2/M arrest and induced apoptosis in the HepG2 cells. USP39 knockdown by shRNA inhibited xenograft tumor growth in nude mice. Moreover, USP39 knockdown led to the upregulation of p-Cdc2 and downregulation of p-Cdc25c and p-myt1, while the expression of total Cdc2, Cdc25c and myt1 was not changed in the USP39-knockdown cells. We also found that p-Cdc2 was decreased in the USP39-overexpressing cells and was upregulated in the xenografted tumors derived from the HepG2/KD cells from nude mice. Meanwhile, the expression levels of FoxM1 and its target genes PLK1 and cyclin B1 were decreased in the USP39-knockdown cells. These results suggest that USP39 may contribute to FoxM1 splicing in HCC tumor cells. Our data indicate that USP39 knockdown inhibited the growth of HCC both in vitro and in vivo through G2/M arrest, which was partly achieved via the inhibition of FoxM1 splicing.
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Copy and paste a formatted citation
Spandidos Publications style
Yuan X, Sun X, Shi X, Jiang C, Yu D, Zhang W, Guan W, Zhou J, Wu Y, Qiu Y, Qiu Y, et al: USP39 promotes the growth of human hepatocellular carcinoma in vitro and in vivo. Oncol Rep 34: 823-832, 2015.
APA
Yuan, X., Sun, X., Shi, X., Jiang, C., Yu, D., Zhang, W. ... Ding, Y. (2015). USP39 promotes the growth of human hepatocellular carcinoma in vitro and in vivo. Oncology Reports, 34, 823-832. https://doi.org/10.3892/or.2015.4065
MLA
Yuan, X., Sun, X., Shi, X., Jiang, C., Yu, D., Zhang, W., Guan, W., Zhou, J., Wu, Y., Qiu, Y., Ding, Y."USP39 promotes the growth of human hepatocellular carcinoma in vitro and in vivo". Oncology Reports 34.2 (2015): 823-832.
Chicago
Yuan, X., Sun, X., Shi, X., Jiang, C., Yu, D., Zhang, W., Guan, W., Zhou, J., Wu, Y., Qiu, Y., Ding, Y."USP39 promotes the growth of human hepatocellular carcinoma in vitro and in vivo". Oncology Reports 34, no. 2 (2015): 823-832. https://doi.org/10.3892/or.2015.4065
Copy and paste a formatted citation
x
Spandidos Publications style
Yuan X, Sun X, Shi X, Jiang C, Yu D, Zhang W, Guan W, Zhou J, Wu Y, Qiu Y, Qiu Y, et al: USP39 promotes the growth of human hepatocellular carcinoma in vitro and in vivo. Oncol Rep 34: 823-832, 2015.
APA
Yuan, X., Sun, X., Shi, X., Jiang, C., Yu, D., Zhang, W. ... Ding, Y. (2015). USP39 promotes the growth of human hepatocellular carcinoma in vitro and in vivo. Oncology Reports, 34, 823-832. https://doi.org/10.3892/or.2015.4065
MLA
Yuan, X., Sun, X., Shi, X., Jiang, C., Yu, D., Zhang, W., Guan, W., Zhou, J., Wu, Y., Qiu, Y., Ding, Y."USP39 promotes the growth of human hepatocellular carcinoma in vitro and in vivo". Oncology Reports 34.2 (2015): 823-832.
Chicago
Yuan, X., Sun, X., Shi, X., Jiang, C., Yu, D., Zhang, W., Guan, W., Zhou, J., Wu, Y., Qiu, Y., Ding, Y."USP39 promotes the growth of human hepatocellular carcinoma in vitro and in vivo". Oncology Reports 34, no. 2 (2015): 823-832. https://doi.org/10.3892/or.2015.4065
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