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Article

Podoplanin is involved in the prognosis of head and neck squamous cell carcinoma through interaction with VEGF-C

  • Authors:
    • Hyun-Yi Kim
    • Ki-Sang Rha
    • Geun Ae Shim
    • Ju-Hee Kim
    • Jin‑Man Kim
    • Song Mei Huang
    • Bon Seok Koo
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology - Head and Neck Surgery, Cancer Research Institute, Research Institute for Medical Sciences, Chungnam National University College of Medicine, Daejeon 301-721, Republic of Korea, Department of Pathology, Chungnam National University College of Medicine, Daejeon 301-721, Republic of Korea
  • Pages: 833-842
    |
    Published online on: June 16, 2015
       https://doi.org/10.3892/or.2015.4070
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Abstract

We investigated the clinical significance of podoplanin expression in relation to clinicopathological variables in head and neck squamous cell carcinoma (HNSCC), to determine its effectiveness as a marker for high-risk HNSCC patients. Upregulation of podoplanin in HNSCC tissues was examined using immunohistochemistry and clinicopathological analyses. Wound healing and invasion assays were performed using HNSCC cells that were transfected with podoplanin siRNA, podoplanin vector and cotransfection with both the podoplanin vector and VEGF-C siRNA. High podoplanin expression was significantly associated with ~3- and 5-fold increases in the presence of positive lymph node metastasis and poor histological grade, respectively (P<0.05). High levels of podoplanin expression were significantly associated with decreased overall and disease-specific survival rates (P<0.05). Furthermore, upregulation of podoplanin induced cell wound repair activity and invasiveness in the FaDu and SNU-1041 cells, respectively, while downregulation of podoplanin expression through VEGF-C silencing using co-transfection of both the podoplanin vector and VEGF-C siRNA suppressed cell wound healing and invasion abilities in the HNSCC cells. Our findings suggest that high podoplanin expression is associated with aggressive tumor behavior, poor prognosis and metastatic regulation through interaction with VEGF-C, suggesting that podoplanin may be used as a potential prognostic biomarker for HNSCC.
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1 

Parkin DM, Bray F, Ferlay J and Pisani P: Global cancer statistics, 2002. CA Cancer J Clin. 55:74–108. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Forastiere A, Koch W, Trotti A and Sidransky D: Head and neck cancer. N Engl J Med. 345:1890–1900. 2001. View Article : Google Scholar

3 

Capote A, Escorial V, Muñoz-Guerra MF, Rodríguez-Campo FJ, Gamallo C and Naval L: Elective neck dissection in early-stage oral squamous cell carcinoma - does it influence recurrence and survival? Head Neck. 29:3–11. 2007. View Article : Google Scholar

4 

Kowalski LP, Bagietto R, Lara JR, Santos RL, Silva JF Jr and Magrin J: Prognostic significance of the distribution of neck node metastasis from oral carcinoma. Head Neck. 22:207–214. 2000. View Article : Google Scholar : PubMed/NCBI

5 

Lothaire P, de Azambuja E, Dequanter D, Lalami Y, Sotiriou C, Andry G, Castro G Jr and Awada A: Molecular markers of head and neck squamous cell carcinoma: Promising signs in need of prospective evaluation. Head Neck. 28:256–269. 2006. View Article : Google Scholar

6 

Lopes MA, Nikitakis NG, Reynolds MA, Ord RA and Sauk J Jr: Biomarkers predictive of lymph node metastases in oral squamous cell carcinoma. J Oral Maxillofac Surg. 60:142–147; discussion 147–148. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Kahn HJ and Marks A: A new monoclonal antibody, D2-40, for detection of lymphatic invasion in primary tumors. Lab Invest. 82:1255–1257. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Schacht V, Ramirez MI, Hong YK, Hirakawa S, Feng D, Harvey N, Williams M, Dvorak AM, Dvorak HF, Oliver G, et al: T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema. EMBO J. 22:3546–3556. 2003. View Article : Google Scholar : PubMed/NCBI

9 

Durchdewald M, Guinea-Viniegra J, Haag D, Riehl A, Lichter P, Hahn M, Wagner EF, Angel P and Hess J: Podoplanin is a novel fos target gene in skin carcinogenesis. Cancer Res. 68:6877–6883. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Shimada Y, Ishii G, Nagai K, Atsumi N, Fujii S, Yamada A, Yamane Y, Hishida T, Nishimura M, Yoshida J, et al: Expression of podoplanin, CD44, and p63 in squamous cell carcinoma of the lung. Cancer Sci. 100:2054–2059. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Dumoff KL, Chu CS, Harris EE, Holtz D, Xu X, Zhang PJ and Acs G: Low podoplanin expression in pretreatment biopsy material predicts poor prognosis in advanced-stage squamous cell carcinoma of the uterine cervix treated by primary radiation. Mod Pathol. 19:708–716. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Chuang WY, Yeh CJ, Wu YC, Chao YK, Liu YH, Tseng CK, Chang HK, Liu HP and Hsueh C: Tumor cell expression of podoplanin correlates with nodal metastasis in esophageal squamous cell carcinoma. Histol Histopathol. 24:1021–1027. 2009.PubMed/NCBI

13 

Rahadiani N, Ikeda J, Makino T, Tian T, Qiu Y, Mamat S, Wang Y, Doki Y, Aozasa K and Morii E: Tumorigenic role of podoplanin in esophageal squamous-cell carcinoma. Ann Surg Oncol. 17:1311–1323. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Yuan P, Temam S, El-Naggar A, Zhou X, Liu DD, Lee JJ and Mao L: Overexpression of podoplanin in oral cancer and its association with poor clinical outcome. Cancer. 107:563–569. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Kawaguchi H, El-Naggar AK, Papadimitrakopoulou V, Ren H, Fan YH, Feng L, Lee JJ, Kim E, Hong WK, Lippman SM, et al: Podoplanin: A novel marker for oral cancer risk in patients with oral premalignancy. J Clin Oncol. 26:354–360. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Byrd DR, Compton CC, Fritz AG, Greene FL and Trotti A: AJCC Cancer Staging Manual. 7th edition. Springer; New York, NY: 2009

17 

Koo BS, Kim JM, Seo ST, Yoon YH, Kwon KR, Kim SH, Kwon HW, Bae WJ and Lim YC: Upregulation of HGF and c-MET is associated with subclinical central lymph node metastasis in papillary thyroid microcarcinoma. Ann Surg Oncol. 21:2310–2317. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Kang YH, Ji NY, Han SR, Lee CI, Kim JW, Yeom YI, Kim YH, Chun HK, Kim JW, Chung JW, et al: ESM-1 regulates cell growth and metastatic process through activation of NF-κB in colorectal cancer. Cell Signal. 24:1940–1949. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Schoppmann SF, Birner P, Studer P and Breiteneder-Geleff S: Lymphatic microvessel density and lymphovascular invasion assessed by anti-podoplanin immunostaining in human breast cancer. Anticancer Res. 21:2351–2355. 2001.PubMed/NCBI

20 

Raica M, Cimpean AM and Ribatti D: The role of podoplanin in tumor progression and metastasis. Anticancer Res. 28:2997–3006. 2008.PubMed/NCBI

21 

Tomita N, Matsumoto T, Hayashi T, Arakawa A, Sonoue H, Kajiyama Y and Tsurumaru M: Lymphatic invasion according to D2-40 immunostaining is a strong predictor of nodal metastasis in superficial squamous cell carcinoma of the esophagus: Algorithm for risk of nodal metastasis based on lymphatic invasion. Pathol Int. 58:282–287. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Schacht V, Dadras SS, Johnson LA, Jackson DG, Hong YK and Detmar M: Up-regulation of the lymphatic marker podoplanin, a mucin-type transmembrane glycoprotein, in human squamous cell carcinomas and germ cell tumors. Am J Pathol. 166:913–921. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Erovic BM, Neuchrist C, Kandutsch S, Woegerbauer M and Pammer J: CD9 expression on lymphatic vessels in head and neck mucosa. Mod Pathol. 16:1028–1034. 2003. View Article : Google Scholar : PubMed/NCBI

24 

Kato Y, Kaneko M, Sata M, Fujita N, Tsuruo T and Osawa M: Enhanced expression of Aggrus (T1alpha/podoplanin), a platelet-aggregation-inducing factor in lung squamous cell carcinoma. Tumour Biol. 26:195–200. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Kimura N and Kimura I: Podoplanin as a marker for mesothelioma. Pathol Int. 55:83–86. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Shibahara J, Kashima T, Kikuchi Y, Kunita A and Fukayama M: Podoplanin is expressed in subsets of tumors of the central nervous system. Virchows Arch. 448:493–499. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Wicki A, Lehembre F, Wick N, Hantusch B, Kerjaschki D and Christofori G: Tumor invasion in the absence of epithelial-mesenchymal transition: Podoplanin-mediated remodeling of the actin cytoskeleton. Cancer Cell. 9:261–272. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Kreppel M, Scheer M, Drebber U, Ritter L and Zöller JE: Impact of podoplanin expression in oral squamous cell carcinoma: Clinical and histopathologic correlations. Virchows Arch. 456:473–482. 2010. View Article : Google Scholar : PubMed/NCBI

29 

Tong L, Yuan S, Feng F and Zhang H: Role of podoplanin expression in esophageal squamous cell carcinoma: A retrospective study. Dis Esophagus. 25:72–80. 2012. View Article : Google Scholar

30 

Rodrigo JP, García-Carracedo D, González MV, Mancebo G, Fresno MF and García-Pedrero J: Podoplanin expression in the development and progression of laryngeal squamous cell carcinomas. Mol Cancer. 9:482010. View Article : Google Scholar : PubMed/NCBI

31 

Martín-Villar E, Megías D, Castel S, Yurrita MM, Vilaró S and Quintanilla M: Podoplanin binds ERM proteins to activate RhoA and promote epithelial-mesenchymal transition. J Cell Sci. 119:4541–4553. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Cueni LN, Hegyi I, Shin JW, Albinger-Hegyi A, Gruber S, Kunstfeld R, Moch H and Detmar M: Tumor lymphangiogenesis and metastasis to lymph nodes induced by cancer cell expression of podoplanin. Am J Pathol. 177:1004–1016. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Kim H, Rha K, Shim GA, Kim J, Kim JM, Huang SM and Koo BS: Podoplanin is involved in the prognosis of head and neck squamous cell carcinoma through interaction with VEGF-C. Oncol Rep 34: 833-842, 2015.
APA
Kim, H., Rha, K., Shim, G.A., Kim, J., Kim, J., Huang, S.M., & Koo, B.S. (2015). Podoplanin is involved in the prognosis of head and neck squamous cell carcinoma through interaction with VEGF-C. Oncology Reports, 34, 833-842. https://doi.org/10.3892/or.2015.4070
MLA
Kim, H., Rha, K., Shim, G. A., Kim, J., Kim, J., Huang, S. M., Koo, B. S."Podoplanin is involved in the prognosis of head and neck squamous cell carcinoma through interaction with VEGF-C". Oncology Reports 34.2 (2015): 833-842.
Chicago
Kim, H., Rha, K., Shim, G. A., Kim, J., Kim, J., Huang, S. M., Koo, B. S."Podoplanin is involved in the prognosis of head and neck squamous cell carcinoma through interaction with VEGF-C". Oncology Reports 34, no. 2 (2015): 833-842. https://doi.org/10.3892/or.2015.4070
Copy and paste a formatted citation
x
Spandidos Publications style
Kim H, Rha K, Shim GA, Kim J, Kim JM, Huang SM and Koo BS: Podoplanin is involved in the prognosis of head and neck squamous cell carcinoma through interaction with VEGF-C. Oncol Rep 34: 833-842, 2015.
APA
Kim, H., Rha, K., Shim, G.A., Kim, J., Kim, J., Huang, S.M., & Koo, B.S. (2015). Podoplanin is involved in the prognosis of head and neck squamous cell carcinoma through interaction with VEGF-C. Oncology Reports, 34, 833-842. https://doi.org/10.3892/or.2015.4070
MLA
Kim, H., Rha, K., Shim, G. A., Kim, J., Kim, J., Huang, S. M., Koo, B. S."Podoplanin is involved in the prognosis of head and neck squamous cell carcinoma through interaction with VEGF-C". Oncology Reports 34.2 (2015): 833-842.
Chicago
Kim, H., Rha, K., Shim, G. A., Kim, J., Kim, J., Huang, S. M., Koo, B. S."Podoplanin is involved in the prognosis of head and neck squamous cell carcinoma through interaction with VEGF-C". Oncology Reports 34, no. 2 (2015): 833-842. https://doi.org/10.3892/or.2015.4070
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