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Prognostic implication of PTPRH hypomethylation in non-small cell lung cancer

  • Authors:
    • Takashi Sato
    • Kenzo Soejima
    • Eri Arai
    • Junko Hamamoto
    • Hiroyuki Yasuda
    • Daisuke Arai
    • Kota Ishioka
    • Keiko Ohgino
    • Katsuhiko Naoki
    • Takashi Kohno
    • Koji Tsuta
    • Shun-Ichi Watanabe
    • Yae Kanai
    • Tomoko Betsuyaku
  • View Affiliations / Copyright

    Affiliations: Division of Pulmonary Medicine, Department of Internal Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan, Division of Molecular Pathology, National Cancer Center Research Institute, Tokyo 104-0045, Japan, Division of Genome Biology, National Cancer Center Research Institute, Tokyo 104-0045, Japan, Department of Pathology and Clinical Laboratories, Pathology Division, National Cancer Center Hospital, Tokyo 104-0045, Japan, Department of Thoracic Oncology, Thoracic Surgery Division, National Cancer Center Hospital, Tokyo 104-0045, Japan
    Copyright: © Sato et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1137-1145
    |
    Published online on: June 25, 2015
       https://doi.org/10.3892/or.2015.4082
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Abstract

PTPRH is a receptor-type protein tyrosine phosphatase thought to be a potential regulator of tumorigenesis. The aim of the present study was to clarify the significance of PTPRH expression and its regulation by DNA methylation in non-small cell lung cancer (NSCLC), especially in lung adenocarcinoma (LADC). PTPRH mRNA expression was examined in 89 NSCLC and corresponding non-cancerous tissues. The correlation between DNA methylation and PTPRH gene expression was investigated in another cohort that consisted of 145 patients with LADC, a major NSCLC subtype. Gene regulation by DNA methylation was assessed using a DNA methylation inhibitor. PTPRH mRNA expression was significantly upregulated in NSCLC. PTPRH DNA methylation was reduced in LADC samples and inversely correlated with mRNA expression. 5-Aza-2'-deoxycytidine treatment of lung cancer cell lines with low PTPRH expression, restored mRNA PTPRH expression levels. Furthermore, low PTPRH methylation was associated with shorter recurrence-free survival (P=1.64x10-4) and overall survival (P=5.54x10-5). Multivariate analysis revealed that PTPRH DNA methylation was an independent prognostic factor (P=6.88x10-3). It was confirmed that PTPRH is overexpressed in NSCLC. Furthermore, we determined that PTPRH is epigenetically regulated by DNA hypomethylation, with prognostic implications for LADC.
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Copy and paste a formatted citation
Spandidos Publications style
Sato T, Soejima K, Arai E, Hamamoto J, Yasuda H, Arai D, Ishioka K, Ohgino K, Naoki K, Kohno T, Kohno T, et al: Prognostic implication of PTPRH hypomethylation in non-small cell lung cancer. Oncol Rep 34: 1137-1145, 2015.
APA
Sato, T., Soejima, K., Arai, E., Hamamoto, J., Yasuda, H., Arai, D. ... Betsuyaku, T. (2015). Prognostic implication of PTPRH hypomethylation in non-small cell lung cancer. Oncology Reports, 34, 1137-1145. https://doi.org/10.3892/or.2015.4082
MLA
Sato, T., Soejima, K., Arai, E., Hamamoto, J., Yasuda, H., Arai, D., Ishioka, K., Ohgino, K., Naoki, K., Kohno, T., Tsuta, K., Watanabe, S., Kanai, Y., Betsuyaku, T."Prognostic implication of PTPRH hypomethylation in non-small cell lung cancer". Oncology Reports 34.3 (2015): 1137-1145.
Chicago
Sato, T., Soejima, K., Arai, E., Hamamoto, J., Yasuda, H., Arai, D., Ishioka, K., Ohgino, K., Naoki, K., Kohno, T., Tsuta, K., Watanabe, S., Kanai, Y., Betsuyaku, T."Prognostic implication of PTPRH hypomethylation in non-small cell lung cancer". Oncology Reports 34, no. 3 (2015): 1137-1145. https://doi.org/10.3892/or.2015.4082
Copy and paste a formatted citation
x
Spandidos Publications style
Sato T, Soejima K, Arai E, Hamamoto J, Yasuda H, Arai D, Ishioka K, Ohgino K, Naoki K, Kohno T, Kohno T, et al: Prognostic implication of PTPRH hypomethylation in non-small cell lung cancer. Oncol Rep 34: 1137-1145, 2015.
APA
Sato, T., Soejima, K., Arai, E., Hamamoto, J., Yasuda, H., Arai, D. ... Betsuyaku, T. (2015). Prognostic implication of PTPRH hypomethylation in non-small cell lung cancer. Oncology Reports, 34, 1137-1145. https://doi.org/10.3892/or.2015.4082
MLA
Sato, T., Soejima, K., Arai, E., Hamamoto, J., Yasuda, H., Arai, D., Ishioka, K., Ohgino, K., Naoki, K., Kohno, T., Tsuta, K., Watanabe, S., Kanai, Y., Betsuyaku, T."Prognostic implication of PTPRH hypomethylation in non-small cell lung cancer". Oncology Reports 34.3 (2015): 1137-1145.
Chicago
Sato, T., Soejima, K., Arai, E., Hamamoto, J., Yasuda, H., Arai, D., Ishioka, K., Ohgino, K., Naoki, K., Kohno, T., Tsuta, K., Watanabe, S., Kanai, Y., Betsuyaku, T."Prognostic implication of PTPRH hypomethylation in non-small cell lung cancer". Oncology Reports 34, no. 3 (2015): 1137-1145. https://doi.org/10.3892/or.2015.4082
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