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Article

Lentivirus-mediated gene silencing of NOB1 suppresses non-small cell lung cancer cell proliferation

  • Authors:
    • Weiyi Huang
    • Weiqing Zhong
    • Jun Xu
    • Benhua Su
    • Guanghui Huang
    • Jiajun Du
    • Qi Liu
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Provincial Hospital Affiliated to Shandong University, Jinan, Shangdong 250021, P.R. China, Department of Radiology, The Fourth People's Hospital of Jinan, Jinan, Shangdong 250031, P.R. China, Department of Medical Engineering, Provincial Hospital Affiliated to Shandong University, Jinan, Shangdong 250021, P.R. China
  • Pages: 1510-1516
    |
    Published online on: July 14, 2015
       https://doi.org/10.3892/or.2015.4132
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Abstract

NIN/RPN12 binding protein 1 (NOB1p) encoded by NOB1 has been found to be an essential factor in 26S proteasome biogenesis which participates in protein degradation. However, the functions of NOB1 in non-small cell lung cancer cells are largely unknown. In the present study, lentivirus-mediated NOB1 shRNA transfection in two non-small cell lung cancer cell lines (A549 and H1299) was accomplished, as determined by fluorescence imaging. Downregulation of NOB1 expression was confirmed by real-time PCR and western blotting. NOB1 silencing resulted in a significant decline in the proliferation and colony formation capability of non-small cell lung cancer cells. Moreover, flow cytometry showed that A549 cells were arrested in the G0/G1 phase of the cell cycle after NOB1 suppression. Furthermore, depletion of NOB1 resulted in a significant decrease in CDK4 and cyclin D1 expression. These results suggest that NOB1 may act as an important regulator in non-small cell lung cancer growth and could be a therapeutic target of non‑small cell lung cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Huang W, Zhong W, Xu J, Su B, Huang G, Du J and Liu Q: Lentivirus-mediated gene silencing of NOB1 suppresses non-small cell lung cancer cell proliferation. Oncol Rep 34: 1510-1516, 2015.
APA
Huang, W., Zhong, W., Xu, J., Su, B., Huang, G., Du, J., & Liu, Q. (2015). Lentivirus-mediated gene silencing of NOB1 suppresses non-small cell lung cancer cell proliferation. Oncology Reports, 34, 1510-1516. https://doi.org/10.3892/or.2015.4132
MLA
Huang, W., Zhong, W., Xu, J., Su, B., Huang, G., Du, J., Liu, Q."Lentivirus-mediated gene silencing of NOB1 suppresses non-small cell lung cancer cell proliferation". Oncology Reports 34.3 (2015): 1510-1516.
Chicago
Huang, W., Zhong, W., Xu, J., Su, B., Huang, G., Du, J., Liu, Q."Lentivirus-mediated gene silencing of NOB1 suppresses non-small cell lung cancer cell proliferation". Oncology Reports 34, no. 3 (2015): 1510-1516. https://doi.org/10.3892/or.2015.4132
Copy and paste a formatted citation
x
Spandidos Publications style
Huang W, Zhong W, Xu J, Su B, Huang G, Du J and Liu Q: Lentivirus-mediated gene silencing of NOB1 suppresses non-small cell lung cancer cell proliferation. Oncol Rep 34: 1510-1516, 2015.
APA
Huang, W., Zhong, W., Xu, J., Su, B., Huang, G., Du, J., & Liu, Q. (2015). Lentivirus-mediated gene silencing of NOB1 suppresses non-small cell lung cancer cell proliferation. Oncology Reports, 34, 1510-1516. https://doi.org/10.3892/or.2015.4132
MLA
Huang, W., Zhong, W., Xu, J., Su, B., Huang, G., Du, J., Liu, Q."Lentivirus-mediated gene silencing of NOB1 suppresses non-small cell lung cancer cell proliferation". Oncology Reports 34.3 (2015): 1510-1516.
Chicago
Huang, W., Zhong, W., Xu, J., Su, B., Huang, G., Du, J., Liu, Q."Lentivirus-mediated gene silencing of NOB1 suppresses non-small cell lung cancer cell proliferation". Oncology Reports 34, no. 3 (2015): 1510-1516. https://doi.org/10.3892/or.2015.4132
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