Lentivirus-mediated gene silencing of NOB1 suppresses non-small cell lung cancer cell proliferation

  • Authors:
    • Weiyi Huang
    • Weiqing Zhong
    • Jun Xu
    • Benhua Su
    • Guanghui Huang
    • Jiajun Du
    • Qi Liu
  • View Affiliations

  • Published online on: July 14, 2015     https://doi.org/10.3892/or.2015.4132
  • Pages: 1510-1516
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Abstract

NIN/RPN12 binding protein 1 (NOB1p) encoded by NOB1 has been found to be an essential factor in 26S proteasome biogenesis which participates in protein degradation. However, the functions of NOB1 in non-small cell lung cancer cells are largely unknown. In the present study, lentivirus-mediated NOB1 shRNA transfection in two non-small cell lung cancer cell lines (A549 and H1299) was accomplished, as determined by fluorescence imaging. Downregulation of NOB1 expression was confirmed by real-time PCR and western blotting. NOB1 silencing resulted in a significant decline in the proliferation and colony formation capability of non-small cell lung cancer cells. Moreover, flow cytometry showed that A549 cells were arrested in the G0/G1 phase of the cell cycle after NOB1 suppression. Furthermore, depletion of NOB1 resulted in a significant decrease in CDK4 and cyclin D1 expression. These results suggest that NOB1 may act as an important regulator in non-small cell lung cancer growth and could be a therapeutic target of non‑small cell lung cancer.
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September-2015
Volume 34 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Huang W, Zhong W, Xu J, Su B, Huang G, Du J and Liu Q: Lentivirus-mediated gene silencing of NOB1 suppresses non-small cell lung cancer cell proliferation. Oncol Rep 34: 1510-1516, 2015
APA
Huang, W., Zhong, W., Xu, J., Su, B., Huang, G., Du, J., & Liu, Q. (2015). Lentivirus-mediated gene silencing of NOB1 suppresses non-small cell lung cancer cell proliferation. Oncology Reports, 34, 1510-1516. https://doi.org/10.3892/or.2015.4132
MLA
Huang, W., Zhong, W., Xu, J., Su, B., Huang, G., Du, J., Liu, Q."Lentivirus-mediated gene silencing of NOB1 suppresses non-small cell lung cancer cell proliferation". Oncology Reports 34.3 (2015): 1510-1516.
Chicago
Huang, W., Zhong, W., Xu, J., Su, B., Huang, G., Du, J., Liu, Q."Lentivirus-mediated gene silencing of NOB1 suppresses non-small cell lung cancer cell proliferation". Oncology Reports 34, no. 3 (2015): 1510-1516. https://doi.org/10.3892/or.2015.4132