MicroRNA-205 inhibits the proliferation and invasion of breast cancer by regulating AMOT expression

  • Authors:
    • Haige Zhang
    • Qingxia Fan
  • View Affiliations

  • Published online on: July 23, 2015     https://doi.org/10.3892/or.2015.4148
  • Pages: 2163-2170
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Abstract

It has been reported that the expression of angiomotin (AMOT) is upre­gulated in breast cancer. However, the regulatory mechanism remains unknown. In the present study, we aimed to ascertain whether the expression of AMOT is regulated by microRNAs (miRNAs) in breast cancer. In the present study, miR-205 was significantly downregulated in breast cancer samples and it was identified to directly target the 3'-untranslated region (3'-UTR) of AMOT in breast cancer MCF-7 cells by luciferase assay. miR-205 and small interfering RNA (siRNA)-mediated AMOT-knockdown experiments revealed that miR-205 significantly inhibited the proliferation and the invasion of MCF-7 cells through a decrease in the expression of AMOT, yet had no effect on apoptosis. Furthermore, we observed that the overexpression of AMOT partially reversed the inhibitory effect of miR-205 on the growth and the invasion of MCF-7 cells. The data indicated that miR-205 regulated the proliferation and the invasion of breast cancer cells through suppression of AMOT expression, at least partly. Therefore, the disordered decreased expression of miR-205 and the resulting AMOT upregulation contributes to breast carcinogenesis, and miR-205-AMOT represents a new potential therapeutic target for the treatment of breast carcinoma.
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October-2015
Volume 34 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhang H and Fan Q: MicroRNA-205 inhibits the proliferation and invasion of breast cancer by regulating AMOT expression. Oncol Rep 34: 2163-2170, 2015.
APA
Zhang, H., & Fan, Q. (2015). MicroRNA-205 inhibits the proliferation and invasion of breast cancer by regulating AMOT expression. Oncology Reports, 34, 2163-2170. https://doi.org/10.3892/or.2015.4148
MLA
Zhang, H., Fan, Q."MicroRNA-205 inhibits the proliferation and invasion of breast cancer by regulating AMOT expression". Oncology Reports 34.4 (2015): 2163-2170.
Chicago
Zhang, H., Fan, Q."MicroRNA-205 inhibits the proliferation and invasion of breast cancer by regulating AMOT expression". Oncology Reports 34, no. 4 (2015): 2163-2170. https://doi.org/10.3892/or.2015.4148