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Article

HAX-1 inhibits apoptosis in prostate cancer through the suppression of caspase-9 activation

  • Authors:
    • Jiliang Yan
    • Chunyan Ma
    • Jian Cheng
    • Zhengguo Li
    • Chao Liu
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Kaifeng Central Hospital, Kaifeng, Henan 475000, P.R. China, Department of Oncology, Taishan Medical University Affiliated Zouping Hospital, Zouping, Shandong 256200, P.R. China, Department of Developmental Biology, Liaoning Medical University, Jinzhou, Liaoning 121001, P.R. China
  • Pages: 2776-2781
    |
    Published online on: August 13, 2015
       https://doi.org/10.3892/or.2015.4202
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Abstract

HS1 associated protein X-1 (HAX-1), a substrate of Src family tyrosine kinases, plays a critical role in cell apoptosis. However, its functions in prostate cancer remains unclear. The present study explored the role and mechanism of HAX-1 in cancer cell apoptosis. The mRNA and protein levels of HAX-1 in the prostate cancer cell lines PC-3, VCaP and DU145 were assessed. Cell proliferation, apoptosis and caspase-9 activities were assessed in DU145 after HAX-1 siRNA treatment. The mRNA and protein levels of HAX-1 in prostate cancer cell lines PC-3, VCaP and DU145 were significantly higher than those in the primary prostate epithelial cells, and DU145 possess the highest mRNA and protein levels compared to PC-3 and VCaP. When HAX-1 was knocked down in DU145, cell proliferation was significantly decreased, accompanied by a decrease in Ki67 protein expression. Compared with the control and control siRNA groups, HAX-1 siRNA promoted cell apoptosis and caspase-9 activation in DU145. Furthermore, prostate cancer cells co-transfected with HAX-1 and caspase-9 promoted viability and reduced apoptosis. In contract, co-transfection of caspase-9 and HAX-1 siRNA suppressed the cell viability and enhanced apoptosis. In summary, the present study demonstrated that HAX-1 inhibits cell apoptosis through caspase-9 inactivation.
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Copy and paste a formatted citation
Spandidos Publications style
Yan J, Ma C, Cheng J, Li Z and Liu C: HAX-1 inhibits apoptosis in prostate cancer through the suppression of caspase-9 activation. Oncol Rep 34: 2776-2781, 2015.
APA
Yan, J., Ma, C., Cheng, J., Li, Z., & Liu, C. (2015). HAX-1 inhibits apoptosis in prostate cancer through the suppression of caspase-9 activation. Oncology Reports, 34, 2776-2781. https://doi.org/10.3892/or.2015.4202
MLA
Yan, J., Ma, C., Cheng, J., Li, Z., Liu, C."HAX-1 inhibits apoptosis in prostate cancer through the suppression of caspase-9 activation". Oncology Reports 34.5 (2015): 2776-2781.
Chicago
Yan, J., Ma, C., Cheng, J., Li, Z., Liu, C."HAX-1 inhibits apoptosis in prostate cancer through the suppression of caspase-9 activation". Oncology Reports 34, no. 5 (2015): 2776-2781. https://doi.org/10.3892/or.2015.4202
Copy and paste a formatted citation
x
Spandidos Publications style
Yan J, Ma C, Cheng J, Li Z and Liu C: HAX-1 inhibits apoptosis in prostate cancer through the suppression of caspase-9 activation. Oncol Rep 34: 2776-2781, 2015.
APA
Yan, J., Ma, C., Cheng, J., Li, Z., & Liu, C. (2015). HAX-1 inhibits apoptosis in prostate cancer through the suppression of caspase-9 activation. Oncology Reports, 34, 2776-2781. https://doi.org/10.3892/or.2015.4202
MLA
Yan, J., Ma, C., Cheng, J., Li, Z., Liu, C."HAX-1 inhibits apoptosis in prostate cancer through the suppression of caspase-9 activation". Oncology Reports 34.5 (2015): 2776-2781.
Chicago
Yan, J., Ma, C., Cheng, J., Li, Z., Liu, C."HAX-1 inhibits apoptosis in prostate cancer through the suppression of caspase-9 activation". Oncology Reports 34, no. 5 (2015): 2776-2781. https://doi.org/10.3892/or.2015.4202
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