Antitumor effect of COOH-terminal polypeptide of human TERT is associated with the declined expression of hTERT and NF-κB p65 in HeLa cells

Wu,Xian; Chen,Jiasheng; Cao,Ying; Xie,Baoping; Li,Hongwei; Zhou,Pingzheng; Qiu,Yuchang; Pang,Jianxin

doi:10.3892/or.2015.4298

Antitumor effect of COOH-terminal polypeptide of human TERT is associated with the declined expression of hTERT and NF-κB p65 in HeLa cells

Authors:
- Xian Wu
- Jiasheng Chen
- Ying Cao
- Baoping Xie
- Hongwei Li
- Pingzheng Zhou
- Yuchang Qiu
- Jianxin Pang
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Affiliations: Center for Drug Evaluation and Research, School of Pharmaceutical Sciences, Southern Medical University, Baiyun, Guangzhou, Guangdong 510515, P.R. China, School of Biotechnology, Southern Medical University, Baiyun, Guangzhou, Guangdong 510515, P.R. China
Published online on: September 21, 2015 https://doi.org/10.3892/or.2015.4298
Pages: 2909-2916

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Abstract

Human telomerase reverse transcriptase (hTERT) plays an important role in the development of tumors and has been investigated as a potent target for anticancer therapy. In the present study, we constructed a recombinant adenovirus, Ad-EGFP-C197 which was capable of expressing COOH‑terminal polypeptide of hTERT (amino acid 936-1,132, termed as C197 for the reason that it contains 197 amino acids). Infection of HeLa cells with Ad-EGFP-C197 suppressed the activity of telomerase, decreased the expression of hTERT and NF-κB p65, and induced rapid growth delay and apoptosis of HeLa cells in vitro. In nude mice xenografted with HeLa tumors, injection of Ad-EGFP-C197 into the tumor nodule significantly slowed tumor growth and promoted tumor cell apoptosis, as well as reduced the expression of NF-κB p65 in tumor tissues. In the present study, we suggest that the antitumor effect of C197 is associated with the declined expression of hTERT and NF-κB p65. Our results highlight the potential of C197 in tumor therapy.

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