miR-124 suppresses the migration and invasion of glioma cells in vitro via Capn4

  • Authors:
    • Jia-Jun Cai
    • Zeng-Xin Qi
    • Ling-Chao Chen
    • Yu Yao
    • Yan Gong
    • Ying Mao
  • View Affiliations

  • Published online on: October 27, 2015     https://doi.org/10.3892/or.2015.4355
  • Pages: 284-290
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Abstract

miR-124 and Capn4 are aberrantly expressed in glioblastoma multiforme (GBM) tissues. In the present study, we investigated miR-124 and Capn4 expression in GBM tissue specimens. The role of miR-124 and Capn4 in the migration and invasion of glioma cells in vitro was also examined. miR-124 and Capn4 expression in 20 GBM and 6 control brain specimens was examined using RT-qPCR and immuno­blotting. Data from The Cancer Genome Atlas were retrieved. Candidate mRNA target sites of miR-124 were predicted using TargetScan/microRNA and binding was examined using dual luciferase reporter assays. The U87 and U251 cells were transfected with scrambled microRNA, miR-124 mimics and/or pLenti-Capn4 prior to wound‑healing and Transwell invasion assays. Proteins involved in the epithelial-mesenchymal transition were examined using immunoblotting. The results showed that miR-124 was significantly downregulated in GBM tissues. Immunoblotting showed a marked upregulation of Capn4 expression in GBM tissues. The Spearman's correlation analysis revealed a negative association between miR-124 expression and Capn4 protein levels. TargetScan/microRNA predicted the miR-124 binding site in the nucleotide 440-446 region within the Capn4 3'-UTR, which was confirmed by luciferase assays. Wound‑healing and Transwell invasion assays demonstrated that Capn4 downregulation or miR-124 mimics suppressed the migration and invasion of glioma cells. Capn4 downregulation or miR-124 mimics reduced the level of phospho-FAK and MMP2, vimentin and N-cadherin in U87 cells. In conclusion, miR-124 was found to suppress the migration and invasion of glioma cells in vitro via Capn4.
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January-2016
Volume 35 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Cai J, Qi Z, Chen L, Yao Y, Gong Y and Mao Y: miR-124 suppresses the migration and invasion of glioma cells in vitro via Capn4. Oncol Rep 35: 284-290, 2016
APA
Cai, J., Qi, Z., Chen, L., Yao, Y., Gong, Y., & Mao, Y. (2016). miR-124 suppresses the migration and invasion of glioma cells in vitro via Capn4. Oncology Reports, 35, 284-290. https://doi.org/10.3892/or.2015.4355
MLA
Cai, J., Qi, Z., Chen, L., Yao, Y., Gong, Y., Mao, Y."miR-124 suppresses the migration and invasion of glioma cells in vitro via Capn4". Oncology Reports 35.1 (2016): 284-290.
Chicago
Cai, J., Qi, Z., Chen, L., Yao, Y., Gong, Y., Mao, Y."miR-124 suppresses the migration and invasion of glioma cells in vitro via Capn4". Oncology Reports 35, no. 1 (2016): 284-290. https://doi.org/10.3892/or.2015.4355