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Article

Overexpression of lysine-specific demethylase 1 promotes androgen-independent transition of human prostate cancer LNCaP cells through activation of the AR signaling pathway and suppression of the p53 signaling pathway

  • Authors:
    • Xuechao Li
    • Tao Li
    • Dehong Chen
    • Peng Zhang
    • Yarong Song
    • Hongxue Zhu
    • Yajun Xiao
    • Yifei Xing
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China, Department of Urology, Zhongda Hospital, Medical School, Southeast University, Nanjing, Jiangsu 210009, P.R. China, Department of Urology, Xiangyang Central Hospital, Xiangyang, Hubei 441021, P.R. China
  • Pages: 584-592
    |
    Published online on: October 30, 2015
       https://doi.org/10.3892/or.2015.4362
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Abstract

Lysine-specific demethylase 1 (LSD1) is the first defined histone demethylase, and was found to be closely correlated with the development and progression of various types of cancers, including prostate cancer (PCa). Previous research suggests that LSD1 is closely related with cell proliferation, angiogenesis, migration and invasion in PCa. However, it remains to be elucidated whether LSD1 is correlated with androgen‑independent (AI) transition of PCa under androgen‑ablated conditions. The present study aimed to investigate the correlation of LSD1 expression with AI transition of human androgen‑dependent PCa LNCaP cells. Our data showed that LSD1 was overexpressed in human PCa specimens and in AI PCa LNCaP‑AI cells, which were established through a three‑month continuous culture of LNCaP cells in androgen‑deprived medium. Under androgen‑deprived conditions, LNCaP‑AI cells grew perfectly with less apoptosis and G0/G1 cell cycle arrest. Overexpression of LSD1 protected the LNCaP cells from androgen deprivation‑induced apoptosis and G0/G1 arrest, while knockdown of LSD1 drove LNCaP‑AI cells into a higher rate of apoptosis and G0/G1 arrest. Furthermore, LSD1 was found to regulate the androgen receptor (AR) and p53 signaling pathways via demethylation, subsequently influencing apoptosis and cell cycle progression. These findings revealed that overexpression of LSD1 promoted AI transition of PCa LNCaP cells under androgen‑ablated conditions via activation of the AR signaling pathway and suppression of the p53 signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Li X, Li T, Chen D, Zhang P, Song Y, Zhu H, Xiao Y and Xing Y: Overexpression of lysine-specific demethylase 1 promotes androgen-independent transition of human prostate cancer LNCaP cells through activation of the AR signaling pathway and suppression of the p53 signaling pathway. Oncol Rep 35: 584-592, 2016.
APA
Li, X., Li, T., Chen, D., Zhang, P., Song, Y., Zhu, H. ... Xing, Y. (2016). Overexpression of lysine-specific demethylase 1 promotes androgen-independent transition of human prostate cancer LNCaP cells through activation of the AR signaling pathway and suppression of the p53 signaling pathway. Oncology Reports, 35, 584-592. https://doi.org/10.3892/or.2015.4362
MLA
Li, X., Li, T., Chen, D., Zhang, P., Song, Y., Zhu, H., Xiao, Y., Xing, Y."Overexpression of lysine-specific demethylase 1 promotes androgen-independent transition of human prostate cancer LNCaP cells through activation of the AR signaling pathway and suppression of the p53 signaling pathway". Oncology Reports 35.1 (2016): 584-592.
Chicago
Li, X., Li, T., Chen, D., Zhang, P., Song, Y., Zhu, H., Xiao, Y., Xing, Y."Overexpression of lysine-specific demethylase 1 promotes androgen-independent transition of human prostate cancer LNCaP cells through activation of the AR signaling pathway and suppression of the p53 signaling pathway". Oncology Reports 35, no. 1 (2016): 584-592. https://doi.org/10.3892/or.2015.4362
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Li T, Chen D, Zhang P, Song Y, Zhu H, Xiao Y and Xing Y: Overexpression of lysine-specific demethylase 1 promotes androgen-independent transition of human prostate cancer LNCaP cells through activation of the AR signaling pathway and suppression of the p53 signaling pathway. Oncol Rep 35: 584-592, 2016.
APA
Li, X., Li, T., Chen, D., Zhang, P., Song, Y., Zhu, H. ... Xing, Y. (2016). Overexpression of lysine-specific demethylase 1 promotes androgen-independent transition of human prostate cancer LNCaP cells through activation of the AR signaling pathway and suppression of the p53 signaling pathway. Oncology Reports, 35, 584-592. https://doi.org/10.3892/or.2015.4362
MLA
Li, X., Li, T., Chen, D., Zhang, P., Song, Y., Zhu, H., Xiao, Y., Xing, Y."Overexpression of lysine-specific demethylase 1 promotes androgen-independent transition of human prostate cancer LNCaP cells through activation of the AR signaling pathway and suppression of the p53 signaling pathway". Oncology Reports 35.1 (2016): 584-592.
Chicago
Li, X., Li, T., Chen, D., Zhang, P., Song, Y., Zhu, H., Xiao, Y., Xing, Y."Overexpression of lysine-specific demethylase 1 promotes androgen-independent transition of human prostate cancer LNCaP cells through activation of the AR signaling pathway and suppression of the p53 signaling pathway". Oncology Reports 35, no. 1 (2016): 584-592. https://doi.org/10.3892/or.2015.4362
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