Genetic network profiles associated with established resistance to ionizing radiation in acute promyelocytic leukemia cells and their extracellular vesicles

  • Authors:
    • Satoru Monzen
    • Mitsuru Chiba
    • Yoichiro Hosokawa
  • View Affiliations

  • Published online on: December 2, 2015     https://doi.org/10.3892/or.2015.4471
  • Pages: 749-756
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Abstract

Radiation-resistant acute promyelocytic leukemia (APL) cells present challenges to treatment, and the acquisition of resistance to ionizing radiation (IR) is a matter of clinical concern. However, little information is available on the behavior of radio-resistant APL in terms of gene expression profiles and intercellular communication. In this study, cDNA microarray and RT-PCR were used to analyze the intracellular genetic network and extracellular vesicles (EVs), respectively, in the established radio-resistant HL60 (Res-HL60) cell line. Significant changes in the expression of 7,309 known mRNAs were observed in Res-HL60 relative to control. In addition, 7 mRNAs were determined as targets because significant changes in the expression were observed using Ingenuity analysis software, confirming the quantitative RT-PCR. However, EVs from Res-HL60 cells did not include these target molecules. These results suggest that radio-resistant APL is regulated by the expression and suppression of specific molecules, and these molecules are not transferred between cells by EVs.
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February-2016
Volume 35 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Monzen S, Chiba M and Hosokawa Y: Genetic network profiles associated with established resistance to ionizing radiation in acute promyelocytic leukemia cells and their extracellular vesicles. Oncol Rep 35: 749-756, 2016
APA
Monzen, S., Chiba, M., & Hosokawa, Y. (2016). Genetic network profiles associated with established resistance to ionizing radiation in acute promyelocytic leukemia cells and their extracellular vesicles. Oncology Reports, 35, 749-756. https://doi.org/10.3892/or.2015.4471
MLA
Monzen, S., Chiba, M., Hosokawa, Y."Genetic network profiles associated with established resistance to ionizing radiation in acute promyelocytic leukemia cells and their extracellular vesicles". Oncology Reports 35.2 (2016): 749-756.
Chicago
Monzen, S., Chiba, M., Hosokawa, Y."Genetic network profiles associated with established resistance to ionizing radiation in acute promyelocytic leukemia cells and their extracellular vesicles". Oncology Reports 35, no. 2 (2016): 749-756. https://doi.org/10.3892/or.2015.4471