Diagnostic and predictive significance of serum microRNA-7 in esophageal squamous cell carcinoma

  • Authors:
    • Wei Dong
    • Baosheng Li
    • Juan Wang
    • Yipeng Song
    • Zicheng Zhang
    • Chengrui Fu
    • Peiliang Zhang
  • View Affiliations

  • Published online on: December 21, 2015     https://doi.org/10.3892/or.2015.4499
  • Pages: 1449-1456
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

MicroRNA-7 has been reported to participate in tumorigenesis and progression by several signaling pathways in various tumors. However, its potential as a serum diagnostic factor and predictive biomarker for esophageal squamous cell carcinoma (ESCC) has not been studied. Serum samples were collected from 105 pathologically proven ESCC patients and 30 age- and gender-matched healthy controls. All patients were treated with concurrent chemoradiotherapy (CRT). Real‑time polymerase chain reaction was carried out to measure the serum miR-7 expression level. The data were compared among radio-sensitive and radio-resistant groups, and healthy volunteers to elucidate the diagnostic and predictive value of miR-7 expression. Finally, in vitro experiments are used to clarify the mechanisms of the miR-7. In the present study, we found that the serum miR-7 level of ESCC patients was 4.74-fold lower as compared with healthy subjects, indicating that serum miR-7 expression could be an excellent diagnostic factor. The serum miR-7 expression level for these responsive patients was 2.34‑fold higher than that for non-responsive patients, indicating it as a valuable biomarker for predicting treatment response of ESCC patients to concurrent chemoradiation treatment. We also found that miR-7 levels are strongly correlated with tumor length and the status of lymph node metastasis (P<0.05). In contrast, the responsiveness of therapy is significantly correlated with CEA (P<0.05), Cyfra21-1 (P<0.05), serum miR-7 level (P<0.05) and myelosuppression (P<0.01). In addition, the experimental data also suggest that miR-7 can interfere with EGFR mRNA translation. In ESCC patients, serum miR-7 has the potential to serve as a noninvasive biomarker of diagnosis and predicting treatment responses to concurrent chemoradiation therapy. ESCC patients with lower Cyfra21-1 and CEA, higher miR-7 and severe myelosuppression were much more sensitive to CRT. In addition, miR-7 may function by interfering with EGFR mRNA translation, but not degradation.
View Figures
View References

Related Articles

Journal Cover

March-2016
Volume 35 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Dong W, Li B, Wang J, Song Y, Zhang Z, Fu C and Zhang P: Diagnostic and predictive significance of serum microRNA-7 in esophageal squamous cell carcinoma. Oncol Rep 35: 1449-1456, 2016
APA
Dong, W., Li, B., Wang, J., Song, Y., Zhang, Z., Fu, C., & Zhang, P. (2016). Diagnostic and predictive significance of serum microRNA-7 in esophageal squamous cell carcinoma. Oncology Reports, 35, 1449-1456. https://doi.org/10.3892/or.2015.4499
MLA
Dong, W., Li, B., Wang, J., Song, Y., Zhang, Z., Fu, C., Zhang, P."Diagnostic and predictive significance of serum microRNA-7 in esophageal squamous cell carcinoma". Oncology Reports 35.3 (2016): 1449-1456.
Chicago
Dong, W., Li, B., Wang, J., Song, Y., Zhang, Z., Fu, C., Zhang, P."Diagnostic and predictive significance of serum microRNA-7 in esophageal squamous cell carcinoma". Oncology Reports 35, no. 3 (2016): 1449-1456. https://doi.org/10.3892/or.2015.4499