miR-27b is upregulated in cervical carcinogenesis and promotes cell growth and invasion by regulating CDH11 and epithelial-mesenchymal transition

  • Authors:
    • Jihang Yao
    • Boya Deng
    • Le Zheng
    • Lei Dou
    • Yi Guo
    • Kejun Guo
  • View Affiliations

  • Published online on: December 21, 2015     https://doi.org/10.3892/or.2015.4500
  • Pages: 1645-1651
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Abstract

Dysregulation of microRNAs (miRNAs) occurs frequently in cervical carcinogenesis. miRNAs function as tumor-suppressors or oncogenes and are involved in tumor behavior. However, the expression and function of miR-27b in cervical carcinogenesis remain unknown. In the present study, we observed that miR-27b was significantly increased in cervical cancer cells and tissues, and upregulation of miR-27b was negatively associated with its direct target, cadherin 11 (CDH11). Upregulation of miR-27b significantly accelerated the proliferation, cell cycle transition from G1 to S phase, migration and invasion of C33A cells, while downregulation of miR-27b suppressed the proliferation and invasion of HeLa cells. Moreover, CDH11 cDNA transfection impaired the oncogenic effect of miR-27b on cancer cells. Knockdown of CDH11 attenuated the suppressive effect of an miR-27b inhibitor on cervical cancer cells. In addition, we found that CDH11 was involved in miR-27b-induced epithelial-mesenchymal transition (EMT) by regulating expression of E-cadherin, vimentin and N-cadherin. Our results for the first time indicate that miR-27b acting as an oncogene may play an important role in the progression of cervical cancer by modulating CDH11 and EMT.
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March-2016
Volume 35 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yao J, Deng B, Zheng L, Dou L, Guo Y and Guo K: miR-27b is upregulated in cervical carcinogenesis and promotes cell growth and invasion by regulating CDH11 and epithelial-mesenchymal transition. Oncol Rep 35: 1645-1651, 2016
APA
Yao, J., Deng, B., Zheng, L., Dou, L., Guo, Y., & Guo, K. (2016). miR-27b is upregulated in cervical carcinogenesis and promotes cell growth and invasion by regulating CDH11 and epithelial-mesenchymal transition. Oncology Reports, 35, 1645-1651. https://doi.org/10.3892/or.2015.4500
MLA
Yao, J., Deng, B., Zheng, L., Dou, L., Guo, Y., Guo, K."miR-27b is upregulated in cervical carcinogenesis and promotes cell growth and invasion by regulating CDH11 and epithelial-mesenchymal transition". Oncology Reports 35.3 (2016): 1645-1651.
Chicago
Yao, J., Deng, B., Zheng, L., Dou, L., Guo, Y., Guo, K."miR-27b is upregulated in cervical carcinogenesis and promotes cell growth and invasion by regulating CDH11 and epithelial-mesenchymal transition". Oncology Reports 35, no. 3 (2016): 1645-1651. https://doi.org/10.3892/or.2015.4500