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Article

miR-27b is upregulated in cervical carcinogenesis and promotes cell growth and invasion by regulating CDH11 and epithelial-mesenchymal transition

  • Authors:
    • Jihang Yao
    • Boya Deng
    • Le Zheng
    • Lei Dou
    • Yi Guo
    • Kejun Guo
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China
  • Pages: 1645-1651
    |
    Published online on: December 21, 2015
       https://doi.org/10.3892/or.2015.4500
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Abstract

Dysregulation of microRNAs (miRNAs) occurs frequently in cervical carcinogenesis. miRNAs function as tumor-suppressors or oncogenes and are involved in tumor behavior. However, the expression and function of miR-27b in cervical carcinogenesis remain unknown. In the present study, we observed that miR-27b was significantly increased in cervical cancer cells and tissues, and upregulation of miR-27b was negatively associated with its direct target, cadherin 11 (CDH11). Upregulation of miR-27b significantly accelerated the proliferation, cell cycle transition from G1 to S phase, migration and invasion of C33A cells, while downregulation of miR-27b suppressed the proliferation and invasion of HeLa cells. Moreover, CDH11 cDNA transfection impaired the oncogenic effect of miR-27b on cancer cells. Knockdown of CDH11 attenuated the suppressive effect of an miR-27b inhibitor on cervical cancer cells. In addition, we found that CDH11 was involved in miR-27b-induced epithelial-mesenchymal transition (EMT) by regulating expression of E-cadherin, vimentin and N-cadherin. Our results for the first time indicate that miR-27b acting as an oncogene may play an important role in the progression of cervical cancer by modulating CDH11 and EMT.
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1 

Forouzanfar MH, Foreman KJ, Delossantos AM, Lozano R, Lopez AD, Murray CJ and Naghavi M: Breast and cervical cancer in 187 countries between 1980 and 2010: A systematic analysis. Lancet. 378:1461–1484. 2011. View Article : Google Scholar : PubMed/NCBI

2 

American Cancer Society: Cervical Cancer. American Cancer Society; Atlanta, GA: 2013

3 

Waggoner SE: Cervical cancer. Lancet. 361:2217–2225. 2003. View Article : Google Scholar : PubMed/NCBI

4 

He L and Hannon GJ: MicroRNAs: Small RNAs with a big role in gene regulation. Nat Rev Genet. 5:522–531. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Filipowicz W, Bhattacharyya SN and Sonenberg N: Mechanisms of post-transcriptional regulation by microRNAs: Are the answers in sight? Nat Rev Genet. 9:102–114. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Mukherji S, Ebert MS, Zheng GX, Tsang JS, Sharp PA and van Oudenaarden A: MicroRNAs can generate thresholds in target gene expression. Nat Genet. 43:854–859. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Calin GA and Croce CM: MicroRNA signatures in human cancers. Nat Rev Cancer. 6:857–866. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Chen L, Li H, Han L, Zhang K, Wang G, Wang Y, Liu Y, Zheng Y, Jiang T, Pu P, et al: Expression and function of miR-27b in human glioma. Oncol Rep. 26:1617–1621. 2011.PubMed/NCBI

9 

Wang Y, Rathinam R, Walch A and Alahari SK: ST14 (suppression of tumorigenicity 14) gene is a target for miR-27b, and the inhibitory effect of ST14 on cell growth is independent of miR-27b regulation. J Biol Chem. 284:23094–23106. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Ishihara T, Seki N, Inoguchi S, Yoshino H, Tatarano S, Yamada Y, Itesako T, Goto Y, Nishikawa R, Nakagawa M, et al: Expression of the tumor suppressive miRNA-23b/27b cluster is a good prognostic marker in clear cell renal cell carcinoma. J Urol. 192:1822–1830. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Chen C, Ridzon DA, Broomer AJ, Zhou Z, Lee DH, Nguyen JT, Barbisin M, Xu NL, Mahuvakar VR, Andersen MR, et al: Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res. 33:e1792005. View Article : Google Scholar : PubMed/NCBI

12 

Zhuo L, Liu J, Wang B, Gao M and Huang A: Differential miRNA expression profiles in hepatocellular carcinoma cells and drug-resistant sublines. Oncol Rep. 29:555–562. 2013.

13 

Shen S, Sun Q, Liang Z, Cui X, Ren X, Chen H, Zhang X and Zhou Y: A prognostic model of triple-negative breast cancer based on miR-27b-3p and node status. PLoS One. 9:e1006642014. View Article : Google Scholar : PubMed/NCBI

14 

Mozos A, Catasús L, D'Angelo E, Serrano E, Espinosa I, Ferrer I, Pons C and Prat J: The FOXO1-miR27 tandem regulates myometrial invasion in endometrioid endometrial adenocarcinoma. Hum Pathol. 45:942–951. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Jiang J, Lv X, Fan L, Huang G, Zhan Y, Wang M and Lu H: MicroRNA-27b suppresses growth and invasion of NSCLC cells by targeting Sp1. Tumour Biol. 35:10019–10023. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Goto Y, Kojima S, Nishikawa R, Enokida H, Chiyomaru T, Kinoshita T, Nakagawa M, Naya Y, Ichikawa T and Seki N: The microRNA-23b/27b/24-1 cluster is a disease progression marker and tumor suppressor in prostate cancer. Oncotarget. 5:7748–7759. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Findlay VJ, Wang C, Watson DK and Camp ER: Epithelial-to-mesenchymal transition and the cancer stem cell phenotype: Insights from cancer biology with therapeutic implications for colorectal cancer. Cancer Gene Ther. 21:181–187. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Cai ZG, Zhang SM, Zhang H, Zhou YY, Wu HB and Xu XP: Aberrant expression of microRNAs involved in epithelial-mesenchymal transition of HT-29 cell line. Cell Biol Int. 37:669–674. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Susuki D, Kimura S, Naganuma S, Tsuchiyama K, Tanaka T, Kitamura N, Fujieda S and Itoh H: Regulation of microRNA expression by hepatocyte growth factor in human head and neck squamous cell carcinoma. Cancer Sci. 102:2164–2171. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Park YT, Jeong JY, Lee MJ, Kim KI, Kim TH, Kwon YD, Lee C, Kim OJ and An HJ: MicroRNAs overexpressed in ovarian ALDH1-positive cells are associated with chemoresistance. J Ovarian Res. 6:182013. View Article : Google Scholar : PubMed/NCBI

21 

Mu W, Hu C, Zhang H, Qu Z, Cen J, Qiu Z, Li C, Ren H, Li Y, He X, et al: miR-27b synergizes with anticancer drugs via p53 activation and CYP1B1 suppression. Cell Res. 25:477–495. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Nakajima G, Patino-Garcia A, Bruheim S, Xi Y, San Julian M, Lecanda F, Sierrasesumaga L, Müller C, Fodstad O and Ju J: CDH11 expression is associated with survival in patients with osteosarcoma. Cancer Genomics Proteomics. 5:37–42. 2008.PubMed/NCBI

23 

Marino N, Collins JW, Shen C, Caplen NJ, Merchant AS, Gökmen-Polar Y, Goswami CP, Hoshino T, Qian Y, Sledge GW Jr, et al: Identification and validation of genes with expression patterns inverse to multiple metastasis suppressor genes in breast cancer cell lines. Clin Exp Metastasis. 31:771–786. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Delic S, Lottmann N, Jetschke K, Reifenberger G and Riemenschneider MJ: Identification and functional validation of CDH11, PCSK6 and SH3GL3 as novel glioma invasion-associated candidate genes. Neuropathol Appl Neurobiol. 38:201–212. 2012. View Article : Google Scholar

25 

Li L, Ying J, Li H, Zhang Y, Shu X, Fan Y, Tan J, Cao Y, Tsao SW, Srivastava G, et al: The human cadherin 11 is a pro-apoptotic tumor suppressor modulating cell stemness through Wnt/β-catenin signaling and silenced in common carcinomas. Oncogene. 31:3901–3912. 2012. View Article : Google Scholar :

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Copy and paste a formatted citation
Spandidos Publications style
Yao J, Deng B, Zheng L, Dou L, Guo Y and Guo K: miR-27b is upregulated in cervical carcinogenesis and promotes cell growth and invasion by regulating CDH11 and epithelial-mesenchymal transition. Oncol Rep 35: 1645-1651, 2016.
APA
Yao, J., Deng, B., Zheng, L., Dou, L., Guo, Y., & Guo, K. (2016). miR-27b is upregulated in cervical carcinogenesis and promotes cell growth and invasion by regulating CDH11 and epithelial-mesenchymal transition. Oncology Reports, 35, 1645-1651. https://doi.org/10.3892/or.2015.4500
MLA
Yao, J., Deng, B., Zheng, L., Dou, L., Guo, Y., Guo, K."miR-27b is upregulated in cervical carcinogenesis and promotes cell growth and invasion by regulating CDH11 and epithelial-mesenchymal transition". Oncology Reports 35.3 (2016): 1645-1651.
Chicago
Yao, J., Deng, B., Zheng, L., Dou, L., Guo, Y., Guo, K."miR-27b is upregulated in cervical carcinogenesis and promotes cell growth and invasion by regulating CDH11 and epithelial-mesenchymal transition". Oncology Reports 35, no. 3 (2016): 1645-1651. https://doi.org/10.3892/or.2015.4500
Copy and paste a formatted citation
x
Spandidos Publications style
Yao J, Deng B, Zheng L, Dou L, Guo Y and Guo K: miR-27b is upregulated in cervical carcinogenesis and promotes cell growth and invasion by regulating CDH11 and epithelial-mesenchymal transition. Oncol Rep 35: 1645-1651, 2016.
APA
Yao, J., Deng, B., Zheng, L., Dou, L., Guo, Y., & Guo, K. (2016). miR-27b is upregulated in cervical carcinogenesis and promotes cell growth and invasion by regulating CDH11 and epithelial-mesenchymal transition. Oncology Reports, 35, 1645-1651. https://doi.org/10.3892/or.2015.4500
MLA
Yao, J., Deng, B., Zheng, L., Dou, L., Guo, Y., Guo, K."miR-27b is upregulated in cervical carcinogenesis and promotes cell growth and invasion by regulating CDH11 and epithelial-mesenchymal transition". Oncology Reports 35.3 (2016): 1645-1651.
Chicago
Yao, J., Deng, B., Zheng, L., Dou, L., Guo, Y., Guo, K."miR-27b is upregulated in cervical carcinogenesis and promotes cell growth and invasion by regulating CDH11 and epithelial-mesenchymal transition". Oncology Reports 35, no. 3 (2016): 1645-1651. https://doi.org/10.3892/or.2015.4500
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