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Article

Antitumor activity of rhein lysinate against human glioma U87 cells in vitro and in vivo

  • Authors:
    • Jin Liu
    • Ke Zhang
    • Yong-Zhan Zhen
    • Jie Wei
    • Gang Hu
    • Jun-Ling Gao
    • Yan-Xia Tian
    • Ya-Jun Lin
  • View Affiliations / Copyright

    Affiliations: College of Life Sciences, Beijing Normal University, Beijing 100875, P.R. China, Oncology Department, Rizhao City People's Hospital, Rizhao, Shandong 276800, P.R. China, Key Laboratory for Chronic Diseases of Hebei Province, Key Laboratory for Preclinical and Basic Research on Chronic Diseases of Tangshan City, School of Basic Medical Sciences, North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China, The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Chinese Ministry of Health, Beijing 100730, P.R. China
  • Pages: 1711-1717
    |
    Published online on: December 24, 2015
       https://doi.org/10.3892/or.2015.4518
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Abstract

In previous studies, we demonstrated that rhein lysinate (RHL), the salt of rhein and lysine that is easily dissolved in water, inhibited the growth of tumor cells derived from breast and ovarian cancer, hepatocellular carcinoma, cervical cancer and lung carcinoma. Based on these observations, human glioma U87 cells and a xenograft model in BALB/c nude mice were used to examine the antitumor activity of RHL against human glioma. Notably, RHL statistically significantly suppressed the growth of human glioma U87 xenografts in BALB/c nude mice. In vitro, there was a significant reduction in cell proliferation after treatment with RHL in a dose- and time-dependent manner. The overall growth inhibition was correlated with the increase in reactive oxygen species (ROS) production and cell apoptosis. The apoptosis- and cell cycle-related proteins including BAX and Bim were increased, whereas Bcl-2 and cyclin D were decreased in the RHL-treated cells. The results demonstrated that RHL is highly effective against the growth of human glioma U87 xenografts in BALB/c nude mice. The potent antitumor activity of RHL may be mediated through downregulation of Bcl-2 and cyclin D expression and upregulation of BAX and Bim expression.
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Copy and paste a formatted citation
Spandidos Publications style
Liu J, Zhang K, Zhen Y, Wei J, Hu G, Gao J, Tian Y and Lin Y: Antitumor activity of rhein lysinate against human glioma U87 cells in vitro and in vivo. Oncol Rep 35: 1711-1717, 2016.
APA
Liu, J., Zhang, K., Zhen, Y., Wei, J., Hu, G., Gao, J. ... Lin, Y. (2016). Antitumor activity of rhein lysinate against human glioma U87 cells in vitro and in vivo. Oncology Reports, 35, 1711-1717. https://doi.org/10.3892/or.2015.4518
MLA
Liu, J., Zhang, K., Zhen, Y., Wei, J., Hu, G., Gao, J., Tian, Y., Lin, Y."Antitumor activity of rhein lysinate against human glioma U87 cells in vitro and in vivo". Oncology Reports 35.3 (2016): 1711-1717.
Chicago
Liu, J., Zhang, K., Zhen, Y., Wei, J., Hu, G., Gao, J., Tian, Y., Lin, Y."Antitumor activity of rhein lysinate against human glioma U87 cells in vitro and in vivo". Oncology Reports 35, no. 3 (2016): 1711-1717. https://doi.org/10.3892/or.2015.4518
Copy and paste a formatted citation
x
Spandidos Publications style
Liu J, Zhang K, Zhen Y, Wei J, Hu G, Gao J, Tian Y and Lin Y: Antitumor activity of rhein lysinate against human glioma U87 cells in vitro and in vivo. Oncol Rep 35: 1711-1717, 2016.
APA
Liu, J., Zhang, K., Zhen, Y., Wei, J., Hu, G., Gao, J. ... Lin, Y. (2016). Antitumor activity of rhein lysinate against human glioma U87 cells in vitro and in vivo. Oncology Reports, 35, 1711-1717. https://doi.org/10.3892/or.2015.4518
MLA
Liu, J., Zhang, K., Zhen, Y., Wei, J., Hu, G., Gao, J., Tian, Y., Lin, Y."Antitumor activity of rhein lysinate against human glioma U87 cells in vitro and in vivo". Oncology Reports 35.3 (2016): 1711-1717.
Chicago
Liu, J., Zhang, K., Zhen, Y., Wei, J., Hu, G., Gao, J., Tian, Y., Lin, Y."Antitumor activity of rhein lysinate against human glioma U87 cells in vitro and in vivo". Oncology Reports 35, no. 3 (2016): 1711-1717. https://doi.org/10.3892/or.2015.4518
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