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Article

RNA-binding motif protein 5 inhibits the proliferation of cigarette smoke-transformed BEAS-2B cells through cell cycle arrest and apoptosis

  • Authors:
    • Xue-Jiao Lv
    • Yan-Wei Du
    • Yu-Qiu Hao
    • Zhen-Zhong Su
    • Lin Zhang
    • Li-Jing Zhao
    • Jie Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University, Changchun, Jilin 130041, P.R. China, Department of Pathology, College of Basic Medicine, Changchun University of Chinese Medicine, Changchun, Jilin 130117, P.R. China, Department of Pathophysiology, College of Basic Medicine, Jilin University, Changchun, Jilin 130021, P.R. China
  • Pages: 2315-2327
    |
    Published online on: January 12, 2016
       https://doi.org/10.3892/or.2016.4551
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Abstract

Cigarette smoking has been shown to be the most significant risk factor for lung cancer. Recent studies have also indicated that RNA-binding motif protein 5 (RBM5) can modulate apoptosis and suppress tumor growth. The present study focused on the role of RBM5 in the regulation of cigarette smoke extract (CSE)-induced transformation of bronchial epithelial cells into the cancerous phenotype and its mechanism of action. Herein, we exposed normal BEAS-2B cells for 8 days to varying concentrations of CSE or dimethylsulfoxide (DMSO), followed by a recovery period of 2 weeks. Next, the RBM5 protein was overexpressed in these transformed BEAS-2B cells though lentiviral infection. Later, the morphological changes, cell proliferation, cell cycle, apoptosis, invasion and migration were assessed. In addition, we analyzed the role of RBM5 in xenograft growth. The expression of RBM5 along with the genes related to cell cycle regulation, apoptosis and invasion were also examined. Finally, our results revealed that BEAS-2B cells exposed to 100 µg/ml CSE acquired phenotypic changes and formed tumors in nude mice, indicative of their cancerous transformation and had reduced RBM5 expression. Subsequent overexpression of RBM5 in these cells significantly inhibited their proliferation, induced G1/S arrest, triggered apoptosis and inhibited their invasion and migration, including xenograft growth. Thus, we established an in vitro model of CSE-induced cancerous transformation and concluded that RBM5 overexpression inhibited the growth of these transformed cells through cell cycle arrest and induction of apoptosis. Therefore, our study suggests the importance of RBM5 in the pathogenesis of smoking-related cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Lv X, Du Y, Hao Y, Su Z, Zhang L, Zhao L and Zhang J: RNA-binding motif protein 5 inhibits the proliferation of cigarette smoke-transformed BEAS-2B cells through cell cycle arrest and apoptosis. Oncol Rep 35: 2315-2327, 2016.
APA
Lv, X., Du, Y., Hao, Y., Su, Z., Zhang, L., Zhao, L., & Zhang, J. (2016). RNA-binding motif protein 5 inhibits the proliferation of cigarette smoke-transformed BEAS-2B cells through cell cycle arrest and apoptosis. Oncology Reports, 35, 2315-2327. https://doi.org/10.3892/or.2016.4551
MLA
Lv, X., Du, Y., Hao, Y., Su, Z., Zhang, L., Zhao, L., Zhang, J."RNA-binding motif protein 5 inhibits the proliferation of cigarette smoke-transformed BEAS-2B cells through cell cycle arrest and apoptosis". Oncology Reports 35.4 (2016): 2315-2327.
Chicago
Lv, X., Du, Y., Hao, Y., Su, Z., Zhang, L., Zhao, L., Zhang, J."RNA-binding motif protein 5 inhibits the proliferation of cigarette smoke-transformed BEAS-2B cells through cell cycle arrest and apoptosis". Oncology Reports 35, no. 4 (2016): 2315-2327. https://doi.org/10.3892/or.2016.4551
Copy and paste a formatted citation
x
Spandidos Publications style
Lv X, Du Y, Hao Y, Su Z, Zhang L, Zhao L and Zhang J: RNA-binding motif protein 5 inhibits the proliferation of cigarette smoke-transformed BEAS-2B cells through cell cycle arrest and apoptosis. Oncol Rep 35: 2315-2327, 2016.
APA
Lv, X., Du, Y., Hao, Y., Su, Z., Zhang, L., Zhao, L., & Zhang, J. (2016). RNA-binding motif protein 5 inhibits the proliferation of cigarette smoke-transformed BEAS-2B cells through cell cycle arrest and apoptosis. Oncology Reports, 35, 2315-2327. https://doi.org/10.3892/or.2016.4551
MLA
Lv, X., Du, Y., Hao, Y., Su, Z., Zhang, L., Zhao, L., Zhang, J."RNA-binding motif protein 5 inhibits the proliferation of cigarette smoke-transformed BEAS-2B cells through cell cycle arrest and apoptosis". Oncology Reports 35.4 (2016): 2315-2327.
Chicago
Lv, X., Du, Y., Hao, Y., Su, Z., Zhang, L., Zhao, L., Zhang, J."RNA-binding motif protein 5 inhibits the proliferation of cigarette smoke-transformed BEAS-2B cells through cell cycle arrest and apoptosis". Oncology Reports 35, no. 4 (2016): 2315-2327. https://doi.org/10.3892/or.2016.4551
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