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Article

MCG101-induced cancer anorexia-cachexia features altered expression of hypothalamic Nucb2 and Cartpt and increased plasma levels of cocaine- and amphetamine-regulated transcript peptides

  • Authors:
    • Jonathan R. Burgos
    • Britt-Marie Iresjö
    • Ulrika Smedh
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, 413 45 Gothenburg, Sweden
  • Pages: 2425-2430
    |
    Published online on: January 14, 2016
       https://doi.org/10.3892/or.2016.4558
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Abstract

The aim of the present study was to explore central and peripheral host responses to an anorexia-cachexia producing tumor. We focused on neuroendocrine anorexigenic signals in the hypothalamus, brainstem, pituitary and from the tumor per se. Expression of mRNA for corticotropin-releasing hormone (CRH), cocaine- and amphetamine-regulated transcript (CART), nesfatin-1, thyrotropin (TSH) and the TSH receptor were explored. In addition, we examined changes in plasma TSH, CART peptides (CARTp) and serum amyloid P component (SAP). C57BL/6 mice were implanted with MCG101 tumors or sham-treated. A sham-implanted, pair‑fed (PF) group was included to delineate between primary tumor and secondary effects from reduced feeding. Food intake and body weight were measured daily. mRNA levels from microdissected mouse brain samples were assayed using qPCR, and plasma levels were determined using ELISA. MCG101 tumors expectedly induced anorexia and loss of body weight. Tumor-bearing (TB) mice exhibited an increase in nesfatin-1 mRNA as well as a decrease in CART mRNA in the paraventricular area (PVN). The CART mRNA response was secondary to reduced caloric intake whereas nesfatin-1 mRNA appeared to be tumor-specifically induced. In the pituitary, CART and TSH mRNA were upregulated in the TB and PF animals compared to the freely fed controls. Plasma levels for CARTp were significantly elevated in TB but not PF mice whereas levels of TSH were unaffected. The plasma CARTp response was correlated to the degree of inflammation represented by SAP. The increase in nesfatin-1 mRNA in the PVN highlights nesfatin-1 as a plausible candidate for causing tumor-induced anorexia. CART mRNA expression in the PVN is likely an adaptation to reduced caloric intake secondary to a cancer anorexia-cachexia syndrome (CACS)‑inducing tumor. The MCG101 tumor did not express CART mRNA, thus the elevation of plasma CARTp is host derived and likely driven by inflammation.
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Copy and paste a formatted citation
Spandidos Publications style
Burgos JR, Iresjö B and Smedh U: MCG101-induced cancer anorexia-cachexia features altered expression of hypothalamic Nucb2 and Cartpt and increased plasma levels of cocaine- and amphetamine-regulated transcript peptides. Oncol Rep 35: 2425-2430, 2016.
APA
Burgos, J.R., Iresjö, B., & Smedh, U. (2016). MCG101-induced cancer anorexia-cachexia features altered expression of hypothalamic Nucb2 and Cartpt and increased plasma levels of cocaine- and amphetamine-regulated transcript peptides. Oncology Reports, 35, 2425-2430. https://doi.org/10.3892/or.2016.4558
MLA
Burgos, J. R., Iresjö, B., Smedh, U."MCG101-induced cancer anorexia-cachexia features altered expression of hypothalamic Nucb2 and Cartpt and increased plasma levels of cocaine- and amphetamine-regulated transcript peptides". Oncology Reports 35.4 (2016): 2425-2430.
Chicago
Burgos, J. R., Iresjö, B., Smedh, U."MCG101-induced cancer anorexia-cachexia features altered expression of hypothalamic Nucb2 and Cartpt and increased plasma levels of cocaine- and amphetamine-regulated transcript peptides". Oncology Reports 35, no. 4 (2016): 2425-2430. https://doi.org/10.3892/or.2016.4558
Copy and paste a formatted citation
x
Spandidos Publications style
Burgos JR, Iresjö B and Smedh U: MCG101-induced cancer anorexia-cachexia features altered expression of hypothalamic Nucb2 and Cartpt and increased plasma levels of cocaine- and amphetamine-regulated transcript peptides. Oncol Rep 35: 2425-2430, 2016.
APA
Burgos, J.R., Iresjö, B., & Smedh, U. (2016). MCG101-induced cancer anorexia-cachexia features altered expression of hypothalamic Nucb2 and Cartpt and increased plasma levels of cocaine- and amphetamine-regulated transcript peptides. Oncology Reports, 35, 2425-2430. https://doi.org/10.3892/or.2016.4558
MLA
Burgos, J. R., Iresjö, B., Smedh, U."MCG101-induced cancer anorexia-cachexia features altered expression of hypothalamic Nucb2 and Cartpt and increased plasma levels of cocaine- and amphetamine-regulated transcript peptides". Oncology Reports 35.4 (2016): 2425-2430.
Chicago
Burgos, J. R., Iresjö, B., Smedh, U."MCG101-induced cancer anorexia-cachexia features altered expression of hypothalamic Nucb2 and Cartpt and increased plasma levels of cocaine- and amphetamine-regulated transcript peptides". Oncology Reports 35, no. 4 (2016): 2425-2430. https://doi.org/10.3892/or.2016.4558
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