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Article

EGCG induces lung cancer A549 cell apoptosis by regulating Ku70 acetylation

  • Authors:
    • Min Li
    • Jing-Jing Li
    • Qi-Hua Gu
    • Jian An
    • Li-Ming Cao
    • Hua-Ping Yang
    • Cheng-Ping Hu
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
  • Pages: 2339-2347
    |
    Published online on: January 21, 2016
       https://doi.org/10.3892/or.2016.4587
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Abstract

Lung cancer is the leading cause of cancer-related death worldwide. (-)-Epigallocatechin-3-gallate (EGCG) is a potential chemopreventive and therapeutic agent for lung cancer. Induction of apoptosis was examined using Annexin V/PI double staining flow cytometry. Western blot analysis detected the protein expression of cleaved caspase-3, Bax and Bcl-xL. Co-immunoprecipitation was used to detect the interaction of Ku70-Bax and the acetylation status of Ku70. Treatment of A549 cells with EGCG-induced apoptosis via increased expression of cleaved caspase-3 and Bax, but decreased expression of Bcl-xL. EGCG upregulated the K70 acetylation status of A549 cells and downregulated the interaction of Bax-Ku70 in a concentration- and time-dependent manner. The apoptosis-promoting effect of EGCG on A549 cells was obviously weakened, along with strengthening of the Bax-Ku70 interaction, after pCDNA3.1(+)-Ku70 plasmid and pCDNA3.1(+)-Ku70539/542R plasmid transfection. Our results established a role of EGCG in inducing cell apoptosis by suppressing Bax activity. Regulating Ku70 acetylation by EGCG, that block the interaction between Ku70 and Bax, will result in lung cancer cell apoptosis.
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View References

1 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Kushi LH, Doyle C, McCullough M, Rock CL, Demark-Wahnefried W, Bandera EV, Gapstur S, Patel AV, Andrews K and Gansler T; American Cancer Society 2010 Nutrition and Physical Activity Guidelines Advisory Committee: American Cancer Society Guidelines on nutrition and physical activity for cancer prevention: Reducing the risk of cancer with healthy food choices and physical activity. CA Cancer J Clin. 62:30–67. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Jin L, Li C, Xu Y, Wang L, Liu J, Wang D, Hong C, Jiang Z, Ma Y, Chen Q, et al: Epigallocatechin gallate promotes p53 accumulation and activity via the inhibition of MDM2-mediated p53 ubiquitination in human lung cancer cells. Oncol Rep. 29:1983–1990. 2013.PubMed/NCBI

4 

Ma YC, Li C, Gao F, Xu Y, Jiang ZB, Liu JX and Jin LY: Epigallocatechin gallate inhibits the growth of human lung cancer by directly targeting the EGFR signaling pathway. Oncol Rep. 31:1343–1349. 2014.

5 

Lee YH, Kwak J, Choi HK, Choi KC, Kim S, Lee J, Jun W, Park HJ and Yoon HG: EGCG suppresses prostate cancer cell growth modulating acetylation of androgen receptor by anti-histone acetyltransferase activity. Int J Mol Med. 30:69–74. 2012.PubMed/NCBI

6 

Tang Y, Zhao DY, Elliott S, Zhao W, Curiel TJ, Beckman BS and Burow ME: Epigallocatechin-3 gallate induces growth inhibition and apoptosis in human breast cancer cells through survivin suppression. Int J Oncol. 31:705–711. 2007.PubMed/NCBI

7 

Yang CS and Wang ZY: Tea and cancer. J Natl Cancer Inst. 85:1038–1049. 1993. View Article : Google Scholar : PubMed/NCBI

8 

Wu H, Xin Y, Xiao Y and Zhao J: Low-dose docetaxel combined with (−)-epigallocatechin-3-gallate inhibits angiogenesis and tumor growth in nude mice with gastric cancer xenografts. Cancer Biother Radiopharm. 27:204–209. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Zhang G, Wang Y, Zhang Y, Wan X, Li J, Liu K, Wang F, Liu K, Liu Q, Yang C, et al: Anti-cancer activities of tea epigallocatechin-3-gallate in breast cancer patients under radiotherapy. Curr Mol Med. 12:163–176. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Tudoran O, Soritau O, Balacescu O, Balacescu L, Braicu C, Rus M, Gherman C, Virag P, Irimie F and Berindan-Neagoe I: Early transcriptional pattern of angiogenesis induced by EGCG treatment in cervical tumour cells. J Cell Mol Med. 16:520–530. 2012. View Article : Google Scholar

11 

Lu YP, Lou YR, Xie JG, Peng QY, Liao J, Yang CS, Huang MT and Conney AH: Topical applications of caffeine or (−)-epigallocatechin gallate (EGCG) inhibit carcinogenesis and selectively increase apoptosis in UVB-induced skin tumors in mice. Proc Natl Acad Sci USA. 99:12455–12460. 2002. View Article : Google Scholar

12 

Syed DN, Afaq F, Kweon MH, Hadi N, Bhatia N, Spiegelman VS and Mukhtar H: Green tea polyphenol EGCG suppresses cigarette smoke condensate-induced NF-kappaB activation in normal human bronchial epithelial cells. Oncogene. 26:673–682. 2007. View Article : Google Scholar

13 

Lee MH, Han DW, Hyon SH and Park JC: Apoptosis of human fibrosarcoma HT-1080 cells by epigallocatechin-3-O-gallate via induction of p53 and caspases as well as suppression of Bcl-2 and phosphorylated nuclear factor-κB. Apoptosis. 16:75–85. 2011. View Article : Google Scholar

14 

Tsukamoto S, Hirotsu K, Kumazoe M, Goto Y, Sugihara K, Suda T, Tsurudome Y, Suzuki T, Yamashita S, Kim Y, et al: Green tea polyphenol EGCG induces lipid-raft clustering and apoptotic cell death by activating protein kinase Cδ and acid sphingomyelinase through a 67 kDa laminin receptor in multiple myeloma cells. Biochem J. 443:525–534. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Adachi S, Nagao T, Ingolfsson HI, Maxfield FR, Andersen OS, Kopelovich L and Weinstein IB: The inhibitory effect of (−)-epigallocatechin gallate on activation of the epidermal growth factor receptor is associated with altered lipid order in HT29 colon cancer cells. Cancer Res. 67:6493–6501. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Wei LH, Kuo ML, Chen CA, Chou CH, Lai KB, Lee CN and Hsieh CY: Interleukin-6 promotes cervical tumor growth by VEGF-dependent angiogenesis via a STAT3 pathway. Oncogene. 22:1517–1527. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Wang X, Hao MW, Dong K, Lin F, Ren JH and Zhang HZ: Apoptosis induction effects of EGCG in laryngeal squamous cell carcinoma cells through telomerase repression. Arch Pharm Res. 32:1263–1269. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Wang J, Xie Y, Feng Y, Zhang L, Huang X, Shen X and Luo X: (−)-Epigallocatechingallate induces apoptosis in B lymphoma cells via caspase-dependent pathway and Bcl-2 family protein modulation. Int J Oncol. 46:1507–1515. 2015.PubMed/NCBI

19 

Sonoda JI, Ikeda R, Baba Y, Narumi K, Kawachi A, Tomishige E, Nishihara K, Takeda Y, Yamada K, Sato K, et al: Green tea catechin, epigallocatechin-3-gallate, attenuates the cell viability of human non-small-cell lung cancer A549 cells via reducing Bcl-xL expression. Exp Ther Med. 8:59–63. 2014.PubMed/NCBI

20 

Hurwitz JL, Stasik I, Kerr EM, Holohan C, Redmond KM, McLaughlin KM, Busacca S, Barbone D, Broaddus VC, Gray SG, et al: Vorinostat/SAHA-induced apoptosis in malignant mesothelioma is FLIP/caspase 8-dependent and HR23B-independent. Eur J Cancer. 48:1096–1107. 2012. View Article : Google Scholar

21 

Hada M and Kwok RP: Regulation of ku70-bax complex in cells. J Cell Death. 7:11–13. 2014.PubMed/NCBI

22 

Li JJ, Gu QH, Li M, Yang HP, Cao LM and Hu CP: Role of Ku70 and Bax in epigallocatechin-3-gallate-induced apoptosis of A549 cells in vivo. Oncol Lett. 5:101–106. 2013.

23 

Kang HG, Jenabi JM, Liu XF, Reynolds CP, Triche TJ and Sorensen PH: Inhibition of the insulin-like growth factor I receptor by epigallocatechin gallate blocks proliferation and induces the death of Ewing tumor cells. Mol Cancer Ther. 9:1396–1407. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Wu PP, Kuo SC, Huang WW, Yang JS, Lai KC, Chen HJ, Lin KL, Chiu YJ, Huang LJ and Chung JG: (−)-Epigallocatechin gallate induced apoptosis in human adrenal cancer NCI-H295 cells through caspase-dependent and caspase-independent pathway. Anticancer Res. 29:1435–1442. 2009.PubMed/NCBI

25 

Deveraux QL, Schendel SL and Reed JC: Antiapoptotic proteins. The bcl-2 and inhibitor of apoptosis protein families. Cardiol Clin. 19:57–74. 2001. View Article : Google Scholar

26 

Gu Q, Hu C, Chen Q and Xia Y: Tea polyphenols prevent lung from preneoplastic lesions and effect p53 and bcl-2 gene expression in rat lung tissues. Int J Clin Exp Pathol. 6:1523–1531. 2013.PubMed/NCBI

27 

Hastak K, Agarwal MK, Mukhtar H and Agarwal ML: Ablation of either p21 or Bax prevents p53-dependent apoptosis induced by green tea polyphenol epigallocatechin-3-gallate. FASEB J. 19:789–791. 2005.PubMed/NCBI

28 

Sawada M, Sun W, Hayes P, Leskov K, Boothman DA and Matsuyama S: Ku70 suppresses the apoptotic translocation of Bax to mitochondria. Nat Cell Biol. 5:320–329. 2003. View Article : Google Scholar : PubMed/NCBI

29 

Sawada M, Hayes P and Matsuyama S: Cytoprotective membrane-permeable peptides designed from the Bax-binding domain of Ku70. Nat Cell Biol. 5:352–357. 2003. View Article : Google Scholar : PubMed/NCBI

30 

Cohen HY, Lavu S, Bitterman KJ, Hekking B, Imahiyerobo TA, Miller C, Frye R, Ploegh H, Kessler BM and Sinclair DA: Acetylation of the C terminus of Ku70 by CBP and PCAF controls Bax-mediated apoptosis. Mol Cell. 13:627–638. 2004. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li M, Li J, Gu Q, An J, Cao L, Yang H and Hu C: EGCG induces lung cancer A549 cell apoptosis by regulating Ku70 acetylation. Oncol Rep 35: 2339-2347, 2016.
APA
Li, M., Li, J., Gu, Q., An, J., Cao, L., Yang, H., & Hu, C. (2016). EGCG induces lung cancer A549 cell apoptosis by regulating Ku70 acetylation. Oncology Reports, 35, 2339-2347. https://doi.org/10.3892/or.2016.4587
MLA
Li, M., Li, J., Gu, Q., An, J., Cao, L., Yang, H., Hu, C."EGCG induces lung cancer A549 cell apoptosis by regulating Ku70 acetylation". Oncology Reports 35.4 (2016): 2339-2347.
Chicago
Li, M., Li, J., Gu, Q., An, J., Cao, L., Yang, H., Hu, C."EGCG induces lung cancer A549 cell apoptosis by regulating Ku70 acetylation". Oncology Reports 35, no. 4 (2016): 2339-2347. https://doi.org/10.3892/or.2016.4587
Copy and paste a formatted citation
x
Spandidos Publications style
Li M, Li J, Gu Q, An J, Cao L, Yang H and Hu C: EGCG induces lung cancer A549 cell apoptosis by regulating Ku70 acetylation. Oncol Rep 35: 2339-2347, 2016.
APA
Li, M., Li, J., Gu, Q., An, J., Cao, L., Yang, H., & Hu, C. (2016). EGCG induces lung cancer A549 cell apoptosis by regulating Ku70 acetylation. Oncology Reports, 35, 2339-2347. https://doi.org/10.3892/or.2016.4587
MLA
Li, M., Li, J., Gu, Q., An, J., Cao, L., Yang, H., Hu, C."EGCG induces lung cancer A549 cell apoptosis by regulating Ku70 acetylation". Oncology Reports 35.4 (2016): 2339-2347.
Chicago
Li, M., Li, J., Gu, Q., An, J., Cao, L., Yang, H., Hu, C."EGCG induces lung cancer A549 cell apoptosis by regulating Ku70 acetylation". Oncology Reports 35, no. 4 (2016): 2339-2347. https://doi.org/10.3892/or.2016.4587
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