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Article

Emodin inhibits epithelial to mesenchymal transition in epithelial ovarian cancer cells by regulation of GSK-3β/β-catenin/ZEB1 signaling pathway

  • Authors:
    • Chen Hu
    • Taotao Dong
    • Rui Li
    • Jingjing Lu
    • Xuan Wei
    • Peishu Liu
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China
  • Pages: 2027-2034
    |
    Published online on: January 25, 2016
       https://doi.org/10.3892/or.2016.4591
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Abstract

Emodin (EMO) has been shown to possess pleiotropic anticancer capabilities in many types of cancer, including epithelial ovarian cancer (EOC). Inhibitory efficacy of EMO on EOC invasion and migration was previously observed, however, the underlying mechanisms have not been completely elucidated. The present study is aimed to explore the mechanisms. Transwell assay demonstrated that EMO significantly inhibited A2780 and SK-OV-3 cell invasion. Western blot analysis was performed to detect the expression levels of epithelial to mesenchymal transition (EMT)-related markers. We found that EMO treatment dose-dependently upregulated E-cadherin, keratin and downregulated N-cadherin, vimentin, matrix metalloproteinase-9 (MMP-9) and matrix metalloproteinase-2 (MMP-2) to repress EMT. Mechanistically, EMO could inhibit glycogen synthase kinase 3β (GSK-3β) phosphorylation, decrease total β-catenin protein levels and subsequently downregulate transcription factor zinc finger E-box binding homeobox 1 (ZEB1) expression. These effects of EMO were weakened when the cells were pretreated with SB216763, an inhibitor of GSK-3β kinase. Besides, we utilized small interfering RNA (siRNA) to downregulate ZEB1 expression. We found that treatment of ZEB1-knockdown cells with EMO, ZEB1 levels were lowest and cell invasion was weakest but ZEB1 knockdown had no effect on the expression of phospho-Ser9-GSK-3β (p-GSK-3βSer9), β-catenin. In conclusion, our results suggested that EMO inhibited EOC cell invasion by regulation of GSK-3β/β-catenin/ZEB1 signaling pathway to suppress EMT in vitro.
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Copy and paste a formatted citation
Spandidos Publications style
Hu C, Dong T, Li R, Lu J, Wei X and Liu P: Emodin inhibits epithelial to mesenchymal transition in epithelial ovarian cancer cells by regulation of GSK-3β/β-catenin/ZEB1 signaling pathway. Oncol Rep 35: 2027-2034, 2016.
APA
Hu, C., Dong, T., Li, R., Lu, J., Wei, X., & Liu, P. (2016). Emodin inhibits epithelial to mesenchymal transition in epithelial ovarian cancer cells by regulation of GSK-3β/β-catenin/ZEB1 signaling pathway. Oncology Reports, 35, 2027-2034. https://doi.org/10.3892/or.2016.4591
MLA
Hu, C., Dong, T., Li, R., Lu, J., Wei, X., Liu, P."Emodin inhibits epithelial to mesenchymal transition in epithelial ovarian cancer cells by regulation of GSK-3β/β-catenin/ZEB1 signaling pathway". Oncology Reports 35.4 (2016): 2027-2034.
Chicago
Hu, C., Dong, T., Li, R., Lu, J., Wei, X., Liu, P."Emodin inhibits epithelial to mesenchymal transition in epithelial ovarian cancer cells by regulation of GSK-3β/β-catenin/ZEB1 signaling pathway". Oncology Reports 35, no. 4 (2016): 2027-2034. https://doi.org/10.3892/or.2016.4591
Copy and paste a formatted citation
x
Spandidos Publications style
Hu C, Dong T, Li R, Lu J, Wei X and Liu P: Emodin inhibits epithelial to mesenchymal transition in epithelial ovarian cancer cells by regulation of GSK-3β/β-catenin/ZEB1 signaling pathway. Oncol Rep 35: 2027-2034, 2016.
APA
Hu, C., Dong, T., Li, R., Lu, J., Wei, X., & Liu, P. (2016). Emodin inhibits epithelial to mesenchymal transition in epithelial ovarian cancer cells by regulation of GSK-3β/β-catenin/ZEB1 signaling pathway. Oncology Reports, 35, 2027-2034. https://doi.org/10.3892/or.2016.4591
MLA
Hu, C., Dong, T., Li, R., Lu, J., Wei, X., Liu, P."Emodin inhibits epithelial to mesenchymal transition in epithelial ovarian cancer cells by regulation of GSK-3β/β-catenin/ZEB1 signaling pathway". Oncology Reports 35.4 (2016): 2027-2034.
Chicago
Hu, C., Dong, T., Li, R., Lu, J., Wei, X., Liu, P."Emodin inhibits epithelial to mesenchymal transition in epithelial ovarian cancer cells by regulation of GSK-3β/β-catenin/ZEB1 signaling pathway". Oncology Reports 35, no. 4 (2016): 2027-2034. https://doi.org/10.3892/or.2016.4591
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