miR-1284 modulates multidrug resistance of gastric cancer cells by targeting EIF4A1

  • Authors:
    • Wenlong Cao
    • Weiyuan Wei
    • Zexu Zhan
    • Yubo Xie
    • Qiang Xiao
  • View Affiliations

  • Published online on: February 29, 2016     https://doi.org/10.3892/or.2016.4643
  • Pages: 2583-2591
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Abstract

Routine chemotherapy as an important treatment mode often can not be effective because of multidrug resistance (MDR). MicroRNA (miRNA) modulates the expression of a great number of genes, including MDR. In this study, the expression of miR-1284 was reduced in gastric cancer (GC) tissue specimens with metastasis and in vincristine-resistant (VCR) GC SGC7901 cells (SGC-7901/VCR) compared to that in the controls. Recombinant lentiviral vectors with miR-1284 led to the overexpression of miR-1284 mRNA and reversed the chemoresistance of SGC7901/VCR cells, promoted cell cycle arrested at the G0/G1 phase, accelerated drug-induced apoptosis, and decreased migration and invasiveness of SGC-7901/VCR. In addition, the overexpression of miR-1284 sensitized tumors to chemotherapy in vivo. Our data provide combined evidence that miR-1284 can heighten the expression of MYC and reduce the expression of JUN, MMP12, and EIF4A1 that was the direct target. In conclusion, miR-1284 can function as a new regulator to reduce GC MDR cells by targeting EIF4A1.
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May-2016
Volume 35 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Cao W, Wei W, Zhan Z, Xie Y and Xiao Q: miR-1284 modulates multidrug resistance of gastric cancer cells by targeting EIF4A1. Oncol Rep 35: 2583-2591, 2016
APA
Cao, W., Wei, W., Zhan, Z., Xie, Y., & Xiao, Q. (2016). miR-1284 modulates multidrug resistance of gastric cancer cells by targeting EIF4A1. Oncology Reports, 35, 2583-2591. https://doi.org/10.3892/or.2016.4643
MLA
Cao, W., Wei, W., Zhan, Z., Xie, Y., Xiao, Q."miR-1284 modulates multidrug resistance of gastric cancer cells by targeting EIF4A1". Oncology Reports 35.5 (2016): 2583-2591.
Chicago
Cao, W., Wei, W., Zhan, Z., Xie, Y., Xiao, Q."miR-1284 modulates multidrug resistance of gastric cancer cells by targeting EIF4A1". Oncology Reports 35, no. 5 (2016): 2583-2591. https://doi.org/10.3892/or.2016.4643