Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
May-2016 Volume 35 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2016 Volume 35 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Cancer testis antigen SPAG9 is a promising marker for the diagnosis and treatment of lung cancer

  • Authors:
    • Biqiong Ren
    • Xiaobin Wei
    • Guoying Zou
    • Junyu He
    • Guofeng Xu
    • Fei Xu
    • Yiran Huang
    • Haowen Zhu
    • Yong Li
    • Guoan Ma
    • Ping Yu
  • View Affiliations / Copyright

    Affiliations: Department of Immunology, Basic Medical College of Central South University, Changsha, Hunan 410078, P.R. China, Laboratory of The Second People's Hospital of Hunan Province, Changsha, Hunan 410007, P.R. China, Department of Laboratory of Hunan University of Chinese Medicine, Changsha, Hunan 410007, P.R. China
    Copyright: © Ren et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2599-2605
    |
    Published online on: March 1, 2016
       https://doi.org/10.3892/or.2016.4645
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Cancer testis antigen sperm-associated antigen 9 (SPAG9) is highly expressed in many types of cancers. In the present study, to obtain a better understanding of the relevance of SPAG9 in cancer diagnosis and treatment, the expression of SPAG9 mRNA and protein in lung cancer specimens was evaluated by RT-PCR, western blotting and immunohistochemistry. ELISA was used to quantify the SPAG9 autoantibody in the peripheral blood of lung cancer patients. The results showed that the expression of SPAG9 mRNA and protein in the lung cancer tissues was significantly higher than that in the adjacent non-cancerous tissues (P<0.01). The level of the SPAG9 autoantibody in the serum of lung cancer patients was significantly higher than the level in the healthy controls (P<0.001), and the level of the SPAG9 autoantibody in the serum of untreated patients was significantly higher than that in treated patients (P=0.002). SPAG9 IgG antibody levels were significantly lower in treated adenocarcinoma and small cell lung cancer patients than these levels in the untreated patients (P=0.006, P=0.026, respectively), while no statistical difference was found between treated and untreated squamous cell carcinoma patients. Our results suggest that the SPAG9 antibody in serum is a promising marker for the diagnosis of lung cancer, and the level of the humoral immune response to this antigen appears to be related to the type of lung cancer.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Suri A: Cancer testis antigens - their importance in immuno-therapy and in the early detection of cancer. Expert Opin Biol Ther. 6:379–389. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Kawakami Y: New cancer therapy by immunomanipulation: Development of immunotherapy for human melanoma as a model system. Cornea. 19(Suppl 3): S2–S6. 2000. View Article : Google Scholar : PubMed/NCBI

3 

Caballero OL and Chen YT: Cancer/testis (CT) antigens: Potential targets for immunotherapy. Cancer Sci. 100:2014–2021. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Garg M, Chaurasiya D, Rana R, Jagadish N, Kanojia D, Dudha N, Kamran N, Salhan S, Bhatnagar A, Suri S, et al: Sperm-associated antigen 9, a novel cancer testis antigen, is a potential target for immunotherapy in epithelial ovarian cancer. Clin Cancer Res. 13:1421–1428. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Garg M, Kanojia D, Khosla A, Dudha N, Sati S, Chaurasiya D, Jagadish N, Seth A, Kumar R, Gupta S, et al: Sperm-associated antigen 9 is associated with tumor growth, migration, and invasion in renal cell carcinoma. Cancer Res. 68:8240–8248. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Kanojia D, Garg M, Gupta S, Gupta A and Suri A: Sperm-associated antigen 9, a novel biomarker for early detection of breast cancer. Cancer Epidemiol Biomarkers Prev. 18:630–639. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Garg M, Kanojia D, Salhan S, Suri S, Gupta A, Lohiya NK and Suri A: Sperm-associated antigen 9 is a biomarker for early cervical carcinoma. Cancer. 115:2671–2683. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Garg M, Kanojia D, Suri S, Gupta S, Gupta A and Suri A: Sperm-associated antigen 9: A novel diagnostic marker for thyroid cancer. J Clin Endocrinol Metab. 94:4613–4618. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Li H, Peng Y, Niu H, Wu B, Zhang Y, Zhang Y, Bai X and He P: SPAG9 is overexpressed in human prostate cancer and promotes cancer cell proliferation. Tumour Biol. 35:6949–6954. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Yi F, Ni W, Liu W, Pan X, Han X, Yang L, Kong X, Ma R and Chang R: SPAG9 is overexpressed in human astrocytoma and promotes cell proliferation and invasion. Tumour Biol. 34:2849–2855. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Xie C, Fu L, Liu N and Li Q: Overexpression of SPAG9 correlates with poor prognosis and tumor progression in hepatocellular carcinoma. Tumour Biol. 35:7685–7691. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

13 

Hara A and Okayasu I: Cyclooxygenase-2 and inducible nitric oxide synthase expression in human astrocytic gliomas: Correlation with angiogenesis and prognostic significance. Acta Neuropathol. 108:43–48. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Zhong L, Coe SP, Stromberg AJ, Khattar NH, Jett JR and Hirschowitz EA: Profiling tumor-associated antibodies for early detection of non-small cell lung cancer. J Thorac Oncol. 1:513–519. 2006. View Article : Google Scholar

15 

Chapman CJ, Murray A, McElveen JE, Sahin U, Luxemburger U, Türeci O, Wiewrodt R, Barnes AC and Robertson JF: Auto-antibodies in lung cancer: Possibilities for early detection and subsequent cure. Thorax. 63:228–233. 2008. View Article : Google Scholar

16 

Qiu J, Choi G, Li L, Wang H, Pitteri SJ, Pereira-Faca SR, Krasnoselsky AL, Randolph TW, Omenn GS, Edelstein C, et al: Occurrence of autoantibodies to Annexin I, 14-3-3 theta and LAMR1 in prediagnostic lung cancer sera. J Clin Oncol. 26:5060–5066. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Tan HT, Low J, Lim SG and Chung MC: Serum autoantibodies as biomarkers for early cancer detection. FEBS J. 276:6880–6904. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Scanlan MJ, Simpson AJ and Old LJ: The cancer/testis genes: Review, standardization, and commentary. Cancer Immun. 4:12004.PubMed/NCBI

19 

de Visser KE, Korets LV and Coussens LM: De novo carcinogenesis promoted by chronic inflammation is B lymphocyte dependent. Cancer Cell. 7:411–423. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Chen F, Lu Z, Deng J, Han X, Bai J, Liu Q, Xi Y and Zheng J: SPAG9 expression is increased in human prostate cancer and promotes cell motility, invasion and angiogenesis in vitro. Oncol Rep. 32:2533–2540. 2014.PubMed/NCBI

21 

Jiang J, Liu Y, Fang W and Liu F: Sperm associated antigen 9 promotes astrocytoma cell invasion through the upregulation of podocalyxin. Mol Med Rep. 10:417–422. 2014.PubMed/NCBI

22 

Kanojia D, Garg M, Saini S, Agarwal S, Parashar D, Jagadish N, Seth A, Bhatnagar A, Gupta A, Kumar R, et al: Sperm associated antigen 9 plays an important role in bladder transitional cell carcinoma. PLoS One. 8:e813482013. View Article : Google Scholar : PubMed/NCBI

23 

Baser E, Togrul C, Ozgu E, Ayhan S, Caglar M, Erkaya S and Gungor T: Sperm-associated antigen 9 is a promising marker for early diagnosis of endometrial cancer. Asian Pac J Cancer Prev. 14:7635–7638. 2013. View Article : Google Scholar

24 

Wang Y, Dong Q, Miao Y, Fu L, Lin X and Wang E: Clinical significance and biological roles of SPAG9 overexpression in non-small cell lung cancer. Lung Cancer. 81:266–272. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Qiu J and Hanash S: Autoantibody profiling for cancer detection. Clin Lab Med. 29:31–46. 2009. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Ren B, Wei X, Zou G, He J, Xu G, Xu F, Huang Y, Zhu H, Li Y, Ma G, Ma G, et al: Cancer testis antigen SPAG9 is a promising marker for the diagnosis and treatment of lung cancer. Oncol Rep 35: 2599-2605, 2016.
APA
Ren, B., Wei, X., Zou, G., He, J., Xu, G., Xu, F. ... Yu, P. (2016). Cancer testis antigen SPAG9 is a promising marker for the diagnosis and treatment of lung cancer. Oncology Reports, 35, 2599-2605. https://doi.org/10.3892/or.2016.4645
MLA
Ren, B., Wei, X., Zou, G., He, J., Xu, G., Xu, F., Huang, Y., Zhu, H., Li, Y., Ma, G., Yu, P."Cancer testis antigen SPAG9 is a promising marker for the diagnosis and treatment of lung cancer". Oncology Reports 35.5 (2016): 2599-2605.
Chicago
Ren, B., Wei, X., Zou, G., He, J., Xu, G., Xu, F., Huang, Y., Zhu, H., Li, Y., Ma, G., Yu, P."Cancer testis antigen SPAG9 is a promising marker for the diagnosis and treatment of lung cancer". Oncology Reports 35, no. 5 (2016): 2599-2605. https://doi.org/10.3892/or.2016.4645
Copy and paste a formatted citation
x
Spandidos Publications style
Ren B, Wei X, Zou G, He J, Xu G, Xu F, Huang Y, Zhu H, Li Y, Ma G, Ma G, et al: Cancer testis antigen SPAG9 is a promising marker for the diagnosis and treatment of lung cancer. Oncol Rep 35: 2599-2605, 2016.
APA
Ren, B., Wei, X., Zou, G., He, J., Xu, G., Xu, F. ... Yu, P. (2016). Cancer testis antigen SPAG9 is a promising marker for the diagnosis and treatment of lung cancer. Oncology Reports, 35, 2599-2605. https://doi.org/10.3892/or.2016.4645
MLA
Ren, B., Wei, X., Zou, G., He, J., Xu, G., Xu, F., Huang, Y., Zhu, H., Li, Y., Ma, G., Yu, P."Cancer testis antigen SPAG9 is a promising marker for the diagnosis and treatment of lung cancer". Oncology Reports 35.5 (2016): 2599-2605.
Chicago
Ren, B., Wei, X., Zou, G., He, J., Xu, G., Xu, F., Huang, Y., Zhu, H., Li, Y., Ma, G., Yu, P."Cancer testis antigen SPAG9 is a promising marker for the diagnosis and treatment of lung cancer". Oncology Reports 35, no. 5 (2016): 2599-2605. https://doi.org/10.3892/or.2016.4645
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team