Open Access

Cancer testis antigen SPAG9 is a promising marker for the diagnosis and treatment of lung cancer

  • Authors:
    • Biqiong Ren
    • Xiaobin Wei
    • Guoying Zou
    • Junyu He
    • Guofeng Xu
    • Fei Xu
    • Yiran Huang
    • Haowen Zhu
    • Yong Li
    • Guoan Ma
    • Ping Yu
  • View Affiliations

  • Published online on: March 1, 2016     https://doi.org/10.3892/or.2016.4645
  • Pages: 2599-2605
  • Copyright: © Ren et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cancer testis antigen sperm-associated antigen 9 (SPAG9) is highly expressed in many types of cancers. In the present study, to obtain a better understanding of the relevance of SPAG9 in cancer diagnosis and treatment, the expression of SPAG9 mRNA and protein in lung cancer specimens was evaluated by RT-PCR, western blotting and immunohistochemistry. ELISA was used to quantify the SPAG9 autoantibody in the peripheral blood of lung cancer patients. The results showed that the expression of SPAG9 mRNA and protein in the lung cancer tissues was significantly higher than that in the adjacent non-cancerous tissues (P<0.01). The level of the SPAG9 autoantibody in the serum of lung cancer patients was significantly higher than the level in the healthy controls (P<0.001), and the level of the SPAG9 autoantibody in the serum of untreated patients was significantly higher than that in treated patients (P=0.002). SPAG9 IgG antibody levels were significantly lower in treated adenocarcinoma and small cell lung cancer patients than these levels in the untreated patients (P=0.006, P=0.026, respectively), while no statistical difference was found between treated and untreated squamous cell carcinoma patients. Our results suggest that the SPAG9 antibody in serum is a promising marker for the diagnosis of lung cancer, and the level of the humoral immune response to this antigen appears to be related to the type of lung cancer.
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May-2016
Volume 35 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ren B, Wei X, Zou G, He J, Xu G, Xu F, Huang Y, Zhu H, Li Y, Ma G, Ma G, et al: Cancer testis antigen SPAG9 is a promising marker for the diagnosis and treatment of lung cancer. Oncol Rep 35: 2599-2605, 2016
APA
Ren, B., Wei, X., Zou, G., He, J., Xu, G., Xu, F. ... Yu, P. (2016). Cancer testis antigen SPAG9 is a promising marker for the diagnosis and treatment of lung cancer. Oncology Reports, 35, 2599-2605. https://doi.org/10.3892/or.2016.4645
MLA
Ren, B., Wei, X., Zou, G., He, J., Xu, G., Xu, F., Huang, Y., Zhu, H., Li, Y., Ma, G., Yu, P."Cancer testis antigen SPAG9 is a promising marker for the diagnosis and treatment of lung cancer". Oncology Reports 35.5 (2016): 2599-2605.
Chicago
Ren, B., Wei, X., Zou, G., He, J., Xu, G., Xu, F., Huang, Y., Zhu, H., Li, Y., Ma, G., Yu, P."Cancer testis antigen SPAG9 is a promising marker for the diagnosis and treatment of lung cancer". Oncology Reports 35, no. 5 (2016): 2599-2605. https://doi.org/10.3892/or.2016.4645