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Article

miR-608 inhibits the migration and invasion of glioma stem cells by targeting macrophage migration inhibitory factor

  • Authors:
    • Zhenhua Wang
    • Yixue Xue
    • Ping Wang
    • Jiaqi Zhu
    • Jun Ma
  • View Affiliations / Copyright

    Affiliations: Department of Physiology, College of Basic Medicine, China Medical University, Shenyang, Liaoning 110122, P.R. China, Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang, Liaoning 110122, P.R. China
  • Pages: 2733-2742
    |
    Published online on: March 3, 2016
       https://doi.org/10.3892/or.2016.4652
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Abstract

Glioma stem cells (GSCs) contribute to the malignant biological behavior of these tumors and have also been shown to be resistant to radiation and chemotherapy. Recently, a variety of microRNAs (miRNAs) has been found to present altered expression and to play an important oncogenic role or tumor-suppressive function in cancer stem cells (CSCs). microRNA-608 (miR-608) is one of the newly discovered microRNAs, and its biological functions remain unclear. Human macrophage migration inhibitory factor (MIF) is a well known oncogene associated with tumor recurrence and the poor prognosis of gliomas. In the present study, we found that miR-608 negatively regulated the gene expression of MIF at the post‑transcriptional level and plays a tumor-suppressive role by targeting MIF in GSCs. We found that miR-608 expression was significantly downregulated and the expression levels of the MIF gene and protein showed an increase in the GSCs. miR-608 overexpression significantly attenuated the proliferation, migration and invasion, and induced the apoptosis of GSCs. The dual-luciferase reporter system revealed that the 3'UTR of MIF is a direct target of miR-608, and miR-608 negatively regulates the gene expression of MIF at the post-transcriptional level by targeting its 3'UTR. Furthermore, we demonstrated that miR-608 overexpression inhibited the malignant behavior of GSCs by downregulating MIF. Western blot results showed that the inhibition of MIF resulted in the inactivation of the PI3K/AKT and JNK pathways. These results demonstrate that miR-608 acts as a potential tumor suppressor and provide insight into new therapeutic targets for malignant glioma.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Z, Xue Y, Wang P, Zhu J and Ma J: miR-608 inhibits the migration and invasion of glioma stem cells by targeting macrophage migration inhibitory factor. Oncol Rep 35: 2733-2742, 2016.
APA
Wang, Z., Xue, Y., Wang, P., Zhu, J., & Ma, J. (2016). miR-608 inhibits the migration and invasion of glioma stem cells by targeting macrophage migration inhibitory factor. Oncology Reports, 35, 2733-2742. https://doi.org/10.3892/or.2016.4652
MLA
Wang, Z., Xue, Y., Wang, P., Zhu, J., Ma, J."miR-608 inhibits the migration and invasion of glioma stem cells by targeting macrophage migration inhibitory factor". Oncology Reports 35.5 (2016): 2733-2742.
Chicago
Wang, Z., Xue, Y., Wang, P., Zhu, J., Ma, J."miR-608 inhibits the migration and invasion of glioma stem cells by targeting macrophage migration inhibitory factor". Oncology Reports 35, no. 5 (2016): 2733-2742. https://doi.org/10.3892/or.2016.4652
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Z, Xue Y, Wang P, Zhu J and Ma J: miR-608 inhibits the migration and invasion of glioma stem cells by targeting macrophage migration inhibitory factor. Oncol Rep 35: 2733-2742, 2016.
APA
Wang, Z., Xue, Y., Wang, P., Zhu, J., & Ma, J. (2016). miR-608 inhibits the migration and invasion of glioma stem cells by targeting macrophage migration inhibitory factor. Oncology Reports, 35, 2733-2742. https://doi.org/10.3892/or.2016.4652
MLA
Wang, Z., Xue, Y., Wang, P., Zhu, J., Ma, J."miR-608 inhibits the migration and invasion of glioma stem cells by targeting macrophage migration inhibitory factor". Oncology Reports 35.5 (2016): 2733-2742.
Chicago
Wang, Z., Xue, Y., Wang, P., Zhu, J., Ma, J."miR-608 inhibits the migration and invasion of glioma stem cells by targeting macrophage migration inhibitory factor". Oncology Reports 35, no. 5 (2016): 2733-2742. https://doi.org/10.3892/or.2016.4652
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