Goniothalamin induces apoptosis associated with autophagy activation through MAPK signaling in SK-BR-3 cells

  • Authors:
    • Sukanda Innajak
    • Wilawan Mahabusrakum
    • Ramida Watanapokasin
  • View Affiliations

  • Published online on: March 7, 2016     https://doi.org/10.3892/or.2016.4655
  • Pages: 2851-2858
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Abstract

Goniothalamin, a plant bioactive styrly-lactone, possesses many biological activities. In the present study, the anticancer effect of goniothalamin on human breast cancer cell line SK-BR-3 was investigated. The results showed that goniothalamin induced nuclear condensation, DNA fragmentation, apoptotic bodies and mitochondrial dysfunction as determined by JC-1 staining. Goniothalamin also increased the Bax/Bcl-2 ratio and expression of cleaved caspase-7, cleaved caspase-9 and cleaved PARP, but decreased Bcl-2 expression. In addition, goniothalamin induced apoptosis via p-JNK1/2 and p-p38 upregulation and inhibited cell survival via p-ERK1/2 and p-Akt downregulation. Notably, goniothalamin induced autophagy through upregulation of Atg7, Atg12-Atg5 conjugation and LC3II. The increased p-p38 and p-JNK1/2 and decreased p-Akt may lead to autophagy induction. Therefore, goniothalamin promoted apoptosis associated with autophagy induction in SK-BR-3 cells through p-p38 and p-JNK1/2 upregulation and p-Akt downregulation. The present study indicated that goniothalamin may be further used as a potential therapeutic candidate or may offer an alternative treatment for breast cancer.
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May-2016
Volume 35 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Innajak S, Mahabusrakum W and Watanapokasin R: Goniothalamin induces apoptosis associated with autophagy activation through MAPK signaling in SK-BR-3 cells. Oncol Rep 35: 2851-2858, 2016
APA
Innajak, S., Mahabusrakum, W., & Watanapokasin, R. (2016). Goniothalamin induces apoptosis associated with autophagy activation through MAPK signaling in SK-BR-3 cells. Oncology Reports, 35, 2851-2858. https://doi.org/10.3892/or.2016.4655
MLA
Innajak, S., Mahabusrakum, W., Watanapokasin, R."Goniothalamin induces apoptosis associated with autophagy activation through MAPK signaling in SK-BR-3 cells". Oncology Reports 35.5 (2016): 2851-2858.
Chicago
Innajak, S., Mahabusrakum, W., Watanapokasin, R."Goniothalamin induces apoptosis associated with autophagy activation through MAPK signaling in SK-BR-3 cells". Oncology Reports 35, no. 5 (2016): 2851-2858. https://doi.org/10.3892/or.2016.4655