Critical involvement of the α(1,2)-fucosyltransferase in multidrug resistance of human chronic myeloid leukemia

Che,Yuxuan; Ren,Xing; Xu,Liye; Ding,Xiaolei; Zhang,Xuan; Sun,Xiuhua

doi:10.3892/or.2016.4673

Critical involvement of the α(1,2)-fucosyltransferase in multidrug resistance of human chronic myeloid leukemia

Authors:
- Yuxuan Che
- Xing Ren
- Liye Xu
- Xiaolei Ding
- Xuan Zhang
- Xiuhua Sun
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Affiliations: Department of Medical Oncology, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116027, P.R. China, College of Stomatology, Dalian Medical University, Dalian, Liaoning 116027, P.R. China
Published online on: March 11, 2016 https://doi.org/10.3892/or.2016.4673
Pages: 3025-3033

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Abstract

The fucosyltransferases are key enzymes in cell surface antigen synthesis during multidrug resistance (MDR) development. The aim of the present study was to analyze the alteration of α(1,2)-fucosyltransferase involved in MDR development in human chronic myeloid leukemia (CML). FUT1 was overexpressed in three CML/MDR cell lines and peripheral blood mononuclear cells (PBMC) of CML patients. However, no significant changes of FUT2 were observed. The altered levels of FUT1 had a significant impact on the phenotypic variation of MDR of K562 and K562/ADR cells, the activity of EGFR/MAPK pathway and P-glycoprotein (P-gp) expression. Blocking the EGFR/MAPK pathway by its specific inhibitor PD153035 or EGFR small interfering RNA (siRNA) resulted in the reduced MDR of K562/ADR cells. This study indicated that α(1,2)-fucosyltransferase involved in the development of MDR of CML cells probably through FUT1 regulated the activity of EGFR/MAPK signaling pathway and the expression of P-gp.

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