Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
May-2016 Volume 35 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2016 Volume 35 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

PTEN enhances G2/M arrest in etoposide-treated MCF‑7 cells through activation of the ATM pathway

  • Authors:
    • Ruopeng Zhang
    • Li Zhu
    • Lirong Zhang
    • Anli Xu
    • Zhengwei Li
    • Yijuan Xu
    • Pei He
    • Maoqing Wu
    • Fengxiang Wei
    • Chenhong Wang
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Shenzhen Maternity and Child Healthcare Hospital, Affiliated to Southern Medical University, Longgang, Shenzhen, Guangdong 518028, P.R. China, Department of Reproductive Medicine, Affiliated Hospital of Dali University, Dali, Yunnan 671000, P.R. China, Clinical Medicine College of Dali University, Dali, Yunnan 671000, P.R. China, Renal Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA, The Genetics Laboratory, Shenzhen Longgang District Maternity and Child Healthcare Hospital, Longgang, Shenzhen, Guangdong 518028, P.R. China
  • Pages: 2707-2714
    |
    Published online on: March 11, 2016
       https://doi.org/10.3892/or.2016.4674
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

As an effective tumor suppressor, phosphatase and tensin homolog (PTEN) has attracted the increased attention of scientists. Recent studies have shown that PTEN plays unique roles in the DNA damage response (DDR) and can interact with the Chk1 pathway. However, little is known about how PTEN contributes to DDR through the ATM-Chk2 pathway. It is well-known that etoposide induces G2/M arrest in a variety of cell lines, including MCF-7 cells. The DNA damage-induced G2/M arrest results from the activation of protein kinase ataxia telangiectasia mutated (ATM), followed by the activation of Chk2 that subsequently inactivates CDC25C, resulting in G2/M arrest. In the present study, we assessed the contribution of PTEN to the etoposide-induced G2/M cell cycle arrest. PTEN was knocked down in MCF-7 cells by specific shRNA, and the effects of PTEN on the ATM-Chk2 pathway were investigated through various approaches. The results showed that knockdown of PTEN strongly antagonized ATM activation in response to etoposide treatment, and thereby reduced the phosphorylation level of ATM substrates, including H2AX, P53 and Chk2. Furthermore, depletion of PTEN reduced the etoposide-induced phosphorylation of CDC25C and strikingly compromised etoposide-induced G2/M arrest in the MCF-7 cells. Altogether, we demonstrated that PTEN plays a unique role in etoposide-induced G2/M arrest by facilitating the activation of the ATM pathway, and PTEN was required for the proper activation of checkpoints in response to DNA damage in MCF-7 cells.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Spryszyńska S, Smok-Pieniążek A, Ferlińska M, Roszak J, Nocuń M and Stępnik M: The influence of ATM, ATR, DNA-PK inhibitors on the cytotoxic and genotoxic effects of dibenzo[def,p]chrysene on human hepatocellular cancer cell line HepG2. Mutat Res Genet Toxicol Environ Mutagen. 791:12–24. 2015. View Article : Google Scholar

2 

Zhang P, Wei Y, Wang L, Debeb BG, Yuan Y, Zhang J, Yuan J, Wang M, Chen D, Sun Y, et al: ATM-mediated stabilization of ZEB1 promotes DNA damage response and radioresistance through CHK1. Nat Cell Biol. 16:864–875. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Benada J and Macurek L: Targeting the checkpoint to kill cancer cells. Biomolecules. 5:1912–1937. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Reddy V, Wu M, Ciavattone N, McKenty N, Menon M, Barrack ER, Reddy GP and Kim SH: ATM inhibition potentiates death of androgen receptor-inactivated prostate cancer cells with telomere dysfunction. J Biol Chem. 290:25522–25533. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Seol HJ, Yoo HY, Jin J, Joo KM, Kong DS, Yoon SJ, Yang H, Kang W, Lim DH, Park K, et al: Prognostic implications of the DNA damage response pathway in glioblastoma. Oncol Rep. 26:423–430. 2011.PubMed/NCBI

6 

Neumann J, Yang Y, Köhler R, Giaisi M, Witzens-Harig M, Liu D, Krammer PH, Lin W and Li-Weber M: Mangrove dolabrane-type of diterpenes tagalsins suppresses tumor growth via ROS-mediated apoptosis and ATM/ATR-Chk1/Chk2-regulated cell cycle arrest. Int J Cancer. 137:2739–2748. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Kwok M, Davies N, Agathanggelou A, Smith E, Petermann E, Yates E, Brown J, Lau A and Stankovic T: Synthetic lethality in chronic lymphocytic leukaemia with DNA damage response defects by targeting the ATR pathway. Lancet. 385(Suppl 1): S582015. View Article : Google Scholar : PubMed/NCBI

8 

Andrs M, Korabecny J, Nepovimova E, Jun D, Hodny Z, Moravcova S, Hanzlikova H and Kuca K: The development of ataxia telangiectasia mutated kinase inhibitors. Mini Rev Med Chem. 14:805–811. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Penedos A, Johnson AL, Strong E, Goldman AS, Carballo JA and Cha RS: Essential and checkpoint functions of budding yeast ATM and ATR during meiotic prophase are facilitated by differential phosphorylation of a meiotic adaptor protein, Hop1. PLoS One. 10:e01342972015. View Article : Google Scholar : PubMed/NCBI

10 

Zhang XP, Liu F and Wang W: Two-phase dynamics of p53 in the DNA damage response. Proc Natl Acad Sci USA. 108:8990–8995. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Palmitelli M, de Campos-Nebel M and González-Cid M: Progression of chromosomal damage induced by etoposide in G2 phase in a DNA-PKcs-deficient context. Chromosome Res. 23:719–732. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Ming M and He YY: PTEN in DNA damage repair. Cancer Lett. 319:125–129. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Ming M, Feng L, Shea CR, Soltani K, Zhao B, Han W, Smart RC, Trempus CS and He YY: PTEN positively regulates UVB-induced DNA damage repair. Cancer Res. 71:5287–5295. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Shen WH, Balajee AS, Wang J, Wu H, Eng C, Pandolfi PP and Yin Y: Essential role for nuclear PTEN in maintaining chromosomal integrity. Cell. 128:157–170. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Rosser CJ, Tanaka M, Pisters LL, Tanaka N, Levy LB, Hoover DC, Grossman HB, McDonnell TJ, Kuban DA and Meyn RE: Adenoviral-mediated PTEN transgene expression sensitizes Bcl-2-expressing prostate cancer cells to radiation. Cancer Gene Ther. 11:273–279. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Pappas G, Zumstein LA, Munshi A, Hobbs M and Meyn RE: Adenoviral-mediated PTEN expression radiosensitizes non-small cell lung cancer cells by suppressing DNA repair capacity. Cancer Gene Ther. 14:543–549. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Gupta A, Yang Q, Pandita RK, Hunt CR, Xiang T, Misri S, Zeng S, Pagan J, Jeffery J, Puc J, et al: Cell cycle checkpoint defects contribute to genomic instability in PTEN deficient cells independent of DNA DSB repair. Cell Cycle. 8:2198–2210. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Paez J and Sellers WR: PI3K/PTEN/AKT pathway. A critical mediator of oncogenic signaling. Cancer Treat Res. 115:145–167. 2003. View Article : Google Scholar : PubMed/NCBI

19 

Lee C, Kim JS and Waldman T: PTEN gene targeting reveals a radiation-induced size checkpoint in human cancer cells. Cancer Res. 64:6906–6914. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Puc J, Keniry M, Li HS, Pandita TK, Choudhury AD, Memeo L, Mansukhani M, Murty VV, Gaciong Z, Meek SE, et al: Lack of PTEN sequesters CHK1 and initiates genetic instability. Cancer Cell. 7:193–204. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Ali IU: Gatekeeper for endometrium: The PTEN tumor suppressor gene. J Natl Cancer Inst. 92:861–863. 2000. View Article : Google Scholar : PubMed/NCBI

22 

Wei F, Xie Y, Tao L and Tang D: Both ERK1 and ERK2 kinases promote G2/M arrest in etoposide-treated MCF7 cells by facilitating ATM activation. Cell Signal. 22:1783–1789. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Nakanishi A, Kitagishi Y, Ogura Y and Matsuda S: The tumor suppressor PTEN interacts with p53 in hereditary cancer (Review). Int J Oncol. 44:1813–1819. 2014.PubMed/NCBI

24 

Conde-Perez A, Gros G, Longvert C, Pedersen M, Petit V, Aktary Z, Viros A, Gesbert F, Delmas V, Rambow F, et al: A caveolin-dependent and PI3K/AKT-independent role of PTEN in β-catenin transcriptional activity. Nat Commun. 6:80932015. View Article : Google Scholar

25 

Puzio-Kuter AM, Laddha SV, Castillo-Martin M, Sun Y, Cordon-Cardo C, Chan CS and Levine AJ: Involvement of tumor suppressors PTEN and p53 in the formation of multiple subtypes of liposarcoma. Cell Death Differ. 22:1785–1791. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Ushio Y, Tada K, Shiraishi S, Kamiryo T, Shinojima N, Kochi M and Saya H: Correlation of molecular genetic analysis of p53, MDM2, p16, PTEN, and EGFR and survival of patients with anaplastic astrocytoma and glioblastoma. Front Biosci. 8:e281–e288. 2003. View Article : Google Scholar : PubMed/NCBI

27 

Zhou M, Gu L, Findley HW, Jiang R and Woods WG: PTEN reverses MDM2-mediated chemotherapy resistance by interacting with p53 in acute lymphoblastic leukemia cells. Cancer Res. 63:6357–6362. 2003.PubMed/NCBI

28 

Bradbury JM and Jackson SP: ATM and ATR. Curr Biol. 13:R4682003. View Article : Google Scholar : PubMed/NCBI

29 

Yang J, Xu ZP, Huang Y, Hamrick HE, Duerksen-Hughes PJ and Yu YN: ATM and ATR: Sensing DNA damage. World J Gastroenterol. 10:155–160. 2004.PubMed/NCBI

30 

Shiloh Y: ATM and ATR: Networking cellular responses to DNA damage. Curr Opin Genet Dev. 11:71–77. 2001. View Article : Google Scholar : PubMed/NCBI

31 

Nam C, Doi K and Nakayama H: Etoposide induces G2/M arrest and apoptosis in neural progenitor cells via DNA damage and an ATM/p53-related pathway. Histol Histopathol. 25:485–493. 2010.PubMed/NCBI

32 

Kim KY, Cho YJ, Jeon GA, Ryu PD and Myeong JN: Membrane-bound alkaline phosphatase gene induces antitumor effect by G2/M arrest in etoposide phosphate-treated cancer cells. Mol Cell Biochem. 252:213–221. 2003. View Article : Google Scholar : PubMed/NCBI

33 

Redon C, Pilch D, Rogakou E, Sedelnikova O, Newrock K and Bonner W: Histone H2A variants H2AX and H2AZ. Curr Opin Genet Dev. 12:162–169. 2002. View Article : Google Scholar : PubMed/NCBI

34 

Sánchez-Flores M, Pásaro E, Bonassi S, Laffon B and Valdiglesias V: γH2AX assay as DNA damage biomarker for human population studies: Defining experimental conditions. Toxicol Sci. 144:406–413. 2015. View Article : Google Scholar

35 

Rao VA, Conti C, Guirouilh-Barbat J, Nakamura A, Miao ZH, Davies SL, Saccá B, Hickson ID, Bensimon A and Pommier Y: Endogenous gamma-H2AX-ATM-Chk2 checkpoint activation in Bloom's syndrome helicase deficient cells is related to DNA replication arrested forks. Mol Cancer Res. 5:713–724. 2007. View Article : Google Scholar : PubMed/NCBI

36 

Savic V, Yin B, Maas NL, Bredemeyer AL, Carpenter AC, Helmink BA, Yang-Iott KS, Sleckman BP and Bassing CH: Formation of dynamic gamma-H2AX domains along broken DNA strands is distinctly regulated by ATM and MDC1 and dependent upon H2AX densities in chromatin. Mol Cell. 34:298–310. 2009. View Article : Google Scholar : PubMed/NCBI

37 

Riballo E, Kühne M, Rief N, Doherty A, Smith GC, Recio MJ, Reis C, Dahm K, Fricke A, Krempler A, et al: A pathway of double-strand break rejoining dependent upon ATM, Artemis, and proteins locating to gamma-H2AX foci. Mol Cell. 16:715–724. 2004. View Article : Google Scholar : PubMed/NCBI

38 

Mah LJ, El-Osta A and Karagiannis TC: gammaH2AX: A sensitive molecular marker of DNA damage and repair. Leukemia. 24:679–686. 2010. View Article : Google Scholar : PubMed/NCBI

39 

Agarwal C, Tyagi A and Agarwal R: Gallic acid causes inactivating phosphorylation of cdc25A/cdc25C-cdc2 via ATM-Chk2 activation, leading to cell cycle arrest, and induces apoptosis in human prostate carcinoma DU145 cells. Mol Cancer Ther. 5:3294–3302. 2006. View Article : Google Scholar : PubMed/NCBI

40 

Ahn J and Prives C: Checkpoint kinase 2 (Chk2) monomers or dimers phosphorylate Cdc25C after DNA damage regardless of threonine 68 phosphorylation. J Biol Chem. 277:48418–48426. 2002. View Article : Google Scholar : PubMed/NCBI

41 

Huang H, Hu M, Zhao R, Li P and Li M: Dihydromyricetin suppresses the proliferation of hepatocellular carcinoma cells by inducing G2/M arrest through the Chk1/Chk2/Cdc25C pathway. Oncol Rep. 30:2467–2475. 2013.PubMed/NCBI

42 

Bahassi M, Myer DL, McKenney RJ, Hennigan RF and Stambrook PJ: Priming phosphorylation of Chk2 by polo-like kinase 3 (Plk3) mediates its full activation by ATM and a downstream checkpoint in response to DNA damage. Mutat Res. 596:166–176. 2006. View Article : Google Scholar

43 

Amir S, Ma AH, Shi XB, Xue L, Kung HJ and Devere White RW: Oncomir miR-125b suppresses p14ARF to modulate p53-dependent and p53-independent apoptosis in prostate cancer. PLoS One. 8:e610642013. View Article : Google Scholar

44 

Mavridis K, Gueugnon F, Petit-Courty A, Courty Y, Barascu A, Guyetant S and Scorilas A: The oncomiR miR-197 is a novel prognostic indicator for non-small cell lung cancer patients. Br J Cancer. 112:1527–1535. 2015. View Article : Google Scholar : PubMed/NCBI

45 

Fletcher CE, Dart DA, Sita-Lumsden A, Cheng H, Rennie PS and Bevan CL: Androgen-regulated processing of the oncomir miR-27a, which targets Prohibitin in prostate cancer. Hum Mol Genet. 21:3112–3127. 2012. View Article : Google Scholar : PubMed/NCBI

46 

Vazquez F and Devreotes P: Regulation of PTEN function as a PIP3 gatekeeper through membrane interaction. Cell Cycle. 5:1523–1527. 2006. View Article : Google Scholar : PubMed/NCBI

47 

Lu Y, Yu Q, Liu JH, Zhang J, Wang H, Koul D, McMurray JS, Fang X, Yung WK, Siminovitch KA, et al: Src family protein-tyrosine kinases alter the function of PTEN to regulate phosphatidylinositol 3-kinase/AKT cascades. J Biol Chem. 278:40057–40066. 2003. View Article : Google Scholar : PubMed/NCBI

48 

Shehata M, Schnabl S, Demirtas D, Hilgarth M, Hubmann R, Ponath E, Badrnya S, Lehner C, Hoelbl A, Duechler M, et al: Reconstitution of PTEN activity by CK2 inhibitors and interference with the PI3-K/Akt cascade counteract the antiapoptotic effect of human stromal cells in chronic lymphocytic leukemia. Blood. 116:2513–2521. 2010. View Article : Google Scholar : PubMed/NCBI

49 

Stocker H, Andjelkovic M, Oldham S, Laffargue M, Wymann MP, Hemmings BA and Hafen E: Living with lethal PIP3 levels: Viability of flies lacking PTEN restored by a PH domain mutation in Akt/PKB. Science. 295:2088–2091. 2002. View Article : Google Scholar : PubMed/NCBI

50 

Yu J, Zhang SS, Saito K, Williams S, Arimura Y, Ma Y, Ke Y, Baron V, Mercola D, Feng GS, et al: PTEN regulation by Akt-EGR1-ARF-PTEN axis. EMBO J. 28:21–33. 2009. View Article : Google Scholar :

51 

Alyasiri NS, Mehdi SJ, Alam MS, Ali A, Mandal AK, Gupta S, Singh I and Rizvi MM: PTEN-mediated AKT activation contributes to the reduced apoptosis among Indian oral squamous cell carcinoma patients. J Cancer Res Clin Oncol. 138:103–109. 2012. View Article : Google Scholar

52 

Abbott RT, Tripp S, Perkins SL, Elenitoba-Johnson KS and Lim MS: Analysis of the PI-3-Kinase-PTEN-AKT pathway in human lymphoma and leukemia using a cell line microarray. Mod Pathol. 16:607–612. 2003. View Article : Google Scholar : PubMed/NCBI

53 

Wen YG, Wang Q, Zhou CZ, Qiu GQ, Peng ZH and Tang HM: Mutation analysis of tumor suppressor gene PTEN in patients with gastric carcinomas and its impact on PI3K/AKT pathway. Oncol Rep. 24:89–95. 2010.PubMed/NCBI

54 

Hande KR: Etoposide: Four decades of development of a topoisomerase II inhibitor. Eur J Cancer. 34:1514–1521. 1998. View Article : Google Scholar

55 

Théard D, Coisy M, Ducommun B, Concannon P and Darbon JM: Etoposide and adriamycin but not genistein can activate the checkpoint kinase Chk2 independently of ATM/ATR. Biochem Biophys Res Commun. 289:1199–1204. 2001. View Article : Google Scholar : PubMed/NCBI

56 

Fu X, Wan S, Lyu YL, Liu LF and Qi H: Etoposide induces ATM-dependent mitochondrial biogenesis through AMPK activation. PLoS One. 3:e20092008. View Article : Google Scholar : PubMed/NCBI

57 

Wei F, Ojo D, Lin X, Wong N, He L, Yan J, Xu S, Major P and Tang D: BMI1 attenuates etoposide-induced G2/M checkpoints via reducing ATM activation. Oncogene. 34:3063–3075. 2015. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang R, Zhu L, Zhang L, Xu A, Li Z, Xu Y, He P, Wu M, Wei F, Wang C, Wang C, et al: PTEN enhances G2/M arrest in etoposide-treated MCF‑7 cells through activation of the ATM pathway. Oncol Rep 35: 2707-2714, 2016.
APA
Zhang, R., Zhu, L., Zhang, L., Xu, A., Li, Z., Xu, Y. ... Wang, C. (2016). PTEN enhances G2/M arrest in etoposide-treated MCF‑7 cells through activation of the ATM pathway. Oncology Reports, 35, 2707-2714. https://doi.org/10.3892/or.2016.4674
MLA
Zhang, R., Zhu, L., Zhang, L., Xu, A., Li, Z., Xu, Y., He, P., Wu, M., Wei, F., Wang, C."PTEN enhances G2/M arrest in etoposide-treated MCF‑7 cells through activation of the ATM pathway". Oncology Reports 35.5 (2016): 2707-2714.
Chicago
Zhang, R., Zhu, L., Zhang, L., Xu, A., Li, Z., Xu, Y., He, P., Wu, M., Wei, F., Wang, C."PTEN enhances G2/M arrest in etoposide-treated MCF‑7 cells through activation of the ATM pathway". Oncology Reports 35, no. 5 (2016): 2707-2714. https://doi.org/10.3892/or.2016.4674
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang R, Zhu L, Zhang L, Xu A, Li Z, Xu Y, He P, Wu M, Wei F, Wang C, Wang C, et al: PTEN enhances G2/M arrest in etoposide-treated MCF‑7 cells through activation of the ATM pathway. Oncol Rep 35: 2707-2714, 2016.
APA
Zhang, R., Zhu, L., Zhang, L., Xu, A., Li, Z., Xu, Y. ... Wang, C. (2016). PTEN enhances G2/M arrest in etoposide-treated MCF‑7 cells through activation of the ATM pathway. Oncology Reports, 35, 2707-2714. https://doi.org/10.3892/or.2016.4674
MLA
Zhang, R., Zhu, L., Zhang, L., Xu, A., Li, Z., Xu, Y., He, P., Wu, M., Wei, F., Wang, C."PTEN enhances G2/M arrest in etoposide-treated MCF‑7 cells through activation of the ATM pathway". Oncology Reports 35.5 (2016): 2707-2714.
Chicago
Zhang, R., Zhu, L., Zhang, L., Xu, A., Li, Z., Xu, Y., He, P., Wu, M., Wei, F., Wang, C."PTEN enhances G2/M arrest in etoposide-treated MCF‑7 cells through activation of the ATM pathway". Oncology Reports 35, no. 5 (2016): 2707-2714. https://doi.org/10.3892/or.2016.4674
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team