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Article

Sedum sarmentosum Bunge extract induces apoptosis and inhibits proliferation in pancreatic cancer cells via the hedgehog signaling pathway

  • Authors:
    • Yongheng Bai
    • Bicheng Chen
    • Weilong Hong
    • Yong Liang
    • Mengtao Zhou
    • Lan Zhou
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Clinical Laboratory Diagnostics, Ministry of Education, College of Clinical Laboratory Diagnostics, Chongqing Medical University, Chongqing 400016, P.R. China, Key Laboratory of Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, Department of Hepatobiliary Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China
  • Pages: 2775-2784
    |
    Published online on: March 16, 2016
       https://doi.org/10.3892/or.2016.4679
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Abstract

Sedum sarmentosum Bunge, a traditional Chinese herbal medicine, has a wide range of clinical applications including antibiosis, anti-inflammation and anti-oxidation. In the present study, we identified that its extract (SSBE) exerts pancreatic anticancer activity in vitro and in vivo. In the cultured pancreatic cancer PANC-1 cell line, SSBE inhibited cell growth in a concentration-dependent manner, and it was accompanied by the downregulated expression of proliferating cell nuclear antigen (PCNA). In addition, SSBE treatment also increased cellular apoptosis in a mitochondrial-dependent manner. Moreover, SSBE induced p53 expression, reduced c-Myc expression, and inhibited epithelial-mesenchymal transition (EMT). The antiproliferative activity of SSBE in the pancreatic cancer cells was found to be closely related to cell cycle arrest at the G2/M phase by upregulating p21(Waf1/CIP1) expression. Further study showed that this inhibitory effect of SSBE was through downregulation of the activity of the proliferation-related Hedgehog signaling pathway. Exogenous recombinant protein Shh was used to activate Hedgehog signaling, thereby resulting in the abolishment of the SSBE-mediated inhibition of pancreatic cancer cell growth. In animal xenograft models of pancreatic cancer, activated Hedgehog signaling was also observed compared with the vehicle controls, but was reduced by SSBE administration. As a result, SSBE suppressed the growth of pancreatic tumors. Thus, these findings demonstrate that SSBE has therapeutic potential for pancreatic cancer, and this anticancer effect in pancreatic cancer cells is associated with inhibition of the Hedgehog signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Bai Y, Chen B, Hong W, Liang Y, Zhou M and Zhou L: Sedum sarmentosum Bunge extract induces apoptosis and inhibits proliferation in pancreatic cancer cells via the hedgehog signaling pathway. Oncol Rep 35: 2775-2784, 2016.
APA
Bai, Y., Chen, B., Hong, W., Liang, Y., Zhou, M., & Zhou, L. (2016). Sedum sarmentosum Bunge extract induces apoptosis and inhibits proliferation in pancreatic cancer cells via the hedgehog signaling pathway. Oncology Reports, 35, 2775-2784. https://doi.org/10.3892/or.2016.4679
MLA
Bai, Y., Chen, B., Hong, W., Liang, Y., Zhou, M., Zhou, L."Sedum sarmentosum Bunge extract induces apoptosis and inhibits proliferation in pancreatic cancer cells via the hedgehog signaling pathway". Oncology Reports 35.5 (2016): 2775-2784.
Chicago
Bai, Y., Chen, B., Hong, W., Liang, Y., Zhou, M., Zhou, L."Sedum sarmentosum Bunge extract induces apoptosis and inhibits proliferation in pancreatic cancer cells via the hedgehog signaling pathway". Oncology Reports 35, no. 5 (2016): 2775-2784. https://doi.org/10.3892/or.2016.4679
Copy and paste a formatted citation
x
Spandidos Publications style
Bai Y, Chen B, Hong W, Liang Y, Zhou M and Zhou L: Sedum sarmentosum Bunge extract induces apoptosis and inhibits proliferation in pancreatic cancer cells via the hedgehog signaling pathway. Oncol Rep 35: 2775-2784, 2016.
APA
Bai, Y., Chen, B., Hong, W., Liang, Y., Zhou, M., & Zhou, L. (2016). Sedum sarmentosum Bunge extract induces apoptosis and inhibits proliferation in pancreatic cancer cells via the hedgehog signaling pathway. Oncology Reports, 35, 2775-2784. https://doi.org/10.3892/or.2016.4679
MLA
Bai, Y., Chen, B., Hong, W., Liang, Y., Zhou, M., Zhou, L."Sedum sarmentosum Bunge extract induces apoptosis and inhibits proliferation in pancreatic cancer cells via the hedgehog signaling pathway". Oncology Reports 35.5 (2016): 2775-2784.
Chicago
Bai, Y., Chen, B., Hong, W., Liang, Y., Zhou, M., Zhou, L."Sedum sarmentosum Bunge extract induces apoptosis and inhibits proliferation in pancreatic cancer cells via the hedgehog signaling pathway". Oncology Reports 35, no. 5 (2016): 2775-2784. https://doi.org/10.3892/or.2016.4679
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