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Article

Hydrogen sulfide promotes cell proliferation of oral cancer through activation of the COX2/AKT/ERK1/2 axis

  • Authors:
    • Shuai Zhang
    • Huan Bian
    • Xiaoxu Li
    • Huanhuan Wu
    • Qingwei Bi
    • Yingbin Yan
    • Yixiang Wang
  • View Affiliations / Copyright

    Affiliations: Central Laboratory, Peking University School and Hospital of Stomatology, Beijing, P.R. China, Department of Stomatology, The First Affiliated Hospital of the Chinese PLA General Hospital, Beijing, P.R. China, Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing, P.R. China, The Second Dental Center, Peking University School and Hospital of Stomatology, Beijing, P.R. China, Department of Oral Surgery, Hospital for Oral Disease Prevention and Treatment, Harbin, Heilongjiang, P.R. China, Department of Oral and Maxillofacial Surgery, Tianjin Stomatological Hospital, Tianjin, P.R. China
  • Pages: 2825-2832
    |
    Published online on: March 17, 2016
       https://doi.org/10.3892/or.2016.4691
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Abstract

Hydrogen sulfide, the third gaseous transmitter, is one of the main causes of halitosis in the oral cavity. It is generally considered as playing a deleterious role in many oral diseases including oral cancer. However, the regulatory mechanisms involved in the effects of hydrogen sulfide on oral cancer growth remain largely unknown. In the present study, we investigated the underlying mechanisms through CCK-8 assay, EdU incorporation, real-time PCR, western blot and pathway blockade assays. Our results showed that hydrogen sulfide promoted oral cancer cell proliferation through activation of the COX2, AKT and ERK1/2 pathways in a dose-dependent manner. Blocking any of the three above pathways inhibited hydrogen sulfide-induced oral cancer cell proliferation. Meanwhile, blockade of COX2 by niflumic acid downregulated NaHS-induced p-ERK and p-AKT expression. Inactivation of the AKT pathway by GSK690693 significantly decreased NaHS‑induced p-ERK1/2 expression, and inhibition of the ERK1/2 pathway by U0126 markedly increased NaHS-induced p-AKT expression. Either the AKT or ERK1/2 inhibitor did not significantly alter the COX2 expression level. Our data revealed, for the first time, that hydrogen sulfide promotes oral cancer cell proliferation through activation of the COX2/AKT/ERK1/2 axis, suggesting new potential targets to eliminate the effect of hydrogen sulfide on the development of oral cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang S, Bian H, Li X, Wu H, Bi Q, Yan Y and Wang Y: Hydrogen sulfide promotes cell proliferation of oral cancer through activation of the COX2/AKT/ERK1/2 axis. Oncol Rep 35: 2825-2832, 2016.
APA
Zhang, S., Bian, H., Li, X., Wu, H., Bi, Q., Yan, Y., & Wang, Y. (2016). Hydrogen sulfide promotes cell proliferation of oral cancer through activation of the COX2/AKT/ERK1/2 axis. Oncology Reports, 35, 2825-2832. https://doi.org/10.3892/or.2016.4691
MLA
Zhang, S., Bian, H., Li, X., Wu, H., Bi, Q., Yan, Y., Wang, Y."Hydrogen sulfide promotes cell proliferation of oral cancer through activation of the COX2/AKT/ERK1/2 axis". Oncology Reports 35.5 (2016): 2825-2832.
Chicago
Zhang, S., Bian, H., Li, X., Wu, H., Bi, Q., Yan, Y., Wang, Y."Hydrogen sulfide promotes cell proliferation of oral cancer through activation of the COX2/AKT/ERK1/2 axis". Oncology Reports 35, no. 5 (2016): 2825-2832. https://doi.org/10.3892/or.2016.4691
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang S, Bian H, Li X, Wu H, Bi Q, Yan Y and Wang Y: Hydrogen sulfide promotes cell proliferation of oral cancer through activation of the COX2/AKT/ERK1/2 axis. Oncol Rep 35: 2825-2832, 2016.
APA
Zhang, S., Bian, H., Li, X., Wu, H., Bi, Q., Yan, Y., & Wang, Y. (2016). Hydrogen sulfide promotes cell proliferation of oral cancer through activation of the COX2/AKT/ERK1/2 axis. Oncology Reports, 35, 2825-2832. https://doi.org/10.3892/or.2016.4691
MLA
Zhang, S., Bian, H., Li, X., Wu, H., Bi, Q., Yan, Y., Wang, Y."Hydrogen sulfide promotes cell proliferation of oral cancer through activation of the COX2/AKT/ERK1/2 axis". Oncology Reports 35.5 (2016): 2825-2832.
Chicago
Zhang, S., Bian, H., Li, X., Wu, H., Bi, Q., Yan, Y., Wang, Y."Hydrogen sulfide promotes cell proliferation of oral cancer through activation of the COX2/AKT/ERK1/2 axis". Oncology Reports 35, no. 5 (2016): 2825-2832. https://doi.org/10.3892/or.2016.4691
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