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Coupling Gd‑DTPA with a bispecific, recombinant protein anti‑EGFR‑iRGD complex improves tumor targeting in MRI

  • Authors:
    • Xiaoyan Xin
    • Huizi Sha
    • Jingtao Shen
    • Bing Zhang
    • Bin Zhu
    • Baorui Liu
  • View Affiliations / Copyright

    Affiliations: Department of Radiology, Affiliated Drum‑Tower Hospital of Nanjing University Medical School, Nanjing 210008, P.R. China, The Comprehensive Cancer Center of Drum‑Tower Hospital, Medical School of Nanjing University and Clinical Cancer Institute of Nanjing University, Nanjing 210008, P.R. China, Department of Nuclear Medicine, Affiliated Drum‑Tower Hospital of Nanjing University Medical School, Nanjing 210008, P.R. China
    Copyright: © Xin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3227-3235
    |
    Published online on: March 29, 2016
       https://doi.org/10.3892/or.2016.4712
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Abstract

Recombinant anti‑epidermal growth factor receptor‑internalizing arginine‑glycine‑aspartic acid (anti‑EGFR single‑domain antibody fused with iRGD peptide) protein efficiently targets the EGFR extracellular domain and integrin αvβ/β5, and shows a high penetration into cells. Thus, this protein may improve penetration of conjugated drugs into the deep zone of gastric cancer multicellular 3D spheroids. In the present study, a novel tumor‑targeting contrast agent for magnetic resonance imaging (MRI) was developed, by coupling gadolinium‑diethylene triamine pentaacetate (Gd‑DTPA) with the bispecific recombinant anti‑EGFR‑iRGD protein. The anti‑EGFR‑iRGD protein was extracted from Escherichia coli and Gd was loaded onto the recombinant protein by chelation using DTPA anhydride. Single‑targeting agent anti‑EGFR‑DTPA‑Gd, which served as the control, was also prepared. The results of the present study showed that anti‑EGFR‑iRGD‑DTPA‑Gd exhibited no significant cyto­toxicity to human gastric carcinoma cells (BGC‑823) under the experimental conditions used. Compared with a conventional contrast agent (Magnevist), anti‑EGFR‑iRGD‑DTPA‑Gd showed higher T1 relaxivity (10.157/mM/sec at 3T) and better tumor‑targeting ability. In addition, the signal intensity and the area under curve for the enhanced signal time in tumor, in vivo, were stronger than Gd‑DTPA alone or the anti‑EGFR‑Gd control. Thus, Gd‑labelled anti‑EGFR‑iRGD has potential as a tumor‑targeting contrast agent for improved MRI.
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Copy and paste a formatted citation
Spandidos Publications style
Xin X, Sha H, Shen J, Zhang B, Zhu B and Liu B: Coupling Gd‑DTPA with a bispecific, recombinant protein anti‑EGFR‑iRGD complex improves tumor targeting in MRI. Oncol Rep 35: 3227-3235, 2016.
APA
Xin, X., Sha, H., Shen, J., Zhang, B., Zhu, B., & Liu, B. (2016). Coupling Gd‑DTPA with a bispecific, recombinant protein anti‑EGFR‑iRGD complex improves tumor targeting in MRI. Oncology Reports, 35, 3227-3235. https://doi.org/10.3892/or.2016.4712
MLA
Xin, X., Sha, H., Shen, J., Zhang, B., Zhu, B., Liu, B."Coupling Gd‑DTPA with a bispecific, recombinant protein anti‑EGFR‑iRGD complex improves tumor targeting in MRI". Oncology Reports 35.6 (2016): 3227-3235.
Chicago
Xin, X., Sha, H., Shen, J., Zhang, B., Zhu, B., Liu, B."Coupling Gd‑DTPA with a bispecific, recombinant protein anti‑EGFR‑iRGD complex improves tumor targeting in MRI". Oncology Reports 35, no. 6 (2016): 3227-3235. https://doi.org/10.3892/or.2016.4712
Copy and paste a formatted citation
x
Spandidos Publications style
Xin X, Sha H, Shen J, Zhang B, Zhu B and Liu B: Coupling Gd‑DTPA with a bispecific, recombinant protein anti‑EGFR‑iRGD complex improves tumor targeting in MRI. Oncol Rep 35: 3227-3235, 2016.
APA
Xin, X., Sha, H., Shen, J., Zhang, B., Zhu, B., & Liu, B. (2016). Coupling Gd‑DTPA with a bispecific, recombinant protein anti‑EGFR‑iRGD complex improves tumor targeting in MRI. Oncology Reports, 35, 3227-3235. https://doi.org/10.3892/or.2016.4712
MLA
Xin, X., Sha, H., Shen, J., Zhang, B., Zhu, B., Liu, B."Coupling Gd‑DTPA with a bispecific, recombinant protein anti‑EGFR‑iRGD complex improves tumor targeting in MRI". Oncology Reports 35.6 (2016): 3227-3235.
Chicago
Xin, X., Sha, H., Shen, J., Zhang, B., Zhu, B., Liu, B."Coupling Gd‑DTPA with a bispecific, recombinant protein anti‑EGFR‑iRGD complex improves tumor targeting in MRI". Oncology Reports 35, no. 6 (2016): 3227-3235. https://doi.org/10.3892/or.2016.4712
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